In vitro system for spermatogenesis from spermatogonial stem cells
| Project/Area Number |
21592080
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YUMURA Yasushi 横浜市立大学, 市民総合医療センター, 講師 (30522023)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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|---|
| Keywords | 精子形成 / 精子幹細胞 / 培養 |
|---|
| Research Abstract |
In vitro reconstitution of spermatogenesis is a very difficult task and remained unsuccessful for a long time. We challenged that task using classical organ culture method, called air-liquid interphase. Through testing variety of culture conditions, we found that a serum replacement, KSR, is effective and induced complete spermatogenesis of mouse. Offspring were obtained with the sperm produced in vitro. We also showed that cryopreservation of testis tissue fragment is possible to preserve spermatogenic ability of the tissues.
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Report
(4 results)
Research Products
(42 results)
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[Presentation] 精原幹細胞と精子形成2010
Author(s)
小川毅彦
Organizer
第13回 RMB研究会シンポジウム
Place of Presentation
持田製薬株式会社「ルークホール」
Year and Date
2010-01-23
Related Report
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