Elucidation of the molecular basis on mammalian gametogenesis by genetic engineering and cell biological techniques

• Project/Area Number: 21592111
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Juntendo University
• Principal Investigator: ARAKI Yoshihiko 順天堂大学, 大学院・医学研究科, 先任准教授 (70250933)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 生殖細胞 / TEX101 / Ly6k / dipeptidase 3 / GPIアンカー型タンパク質 / 精子形成 / マウス / 精巣

Research Abstract

TEX101, a unique GPI-anchored germ cell marker protein, belongs Ly6-like protein superfamily. In this study, we analyzed molecular characteristics of Ly6k and dipeptidase 3, associated molecules with TEX101 in the mouse testis. The experimental results have shown that precise molecular expression of Ly6k during testicular development, and dipeptidase 3 forms complexes with TEX101 on the surfaces of spermatocytes, spermatids, and testicular spermatozoa.

Research Products

• [Journal Article] Gene suppression of mouse testis in vivo using small interfering RNA derived from plasmid vectors (2012)
  • Author(s): Takizawa T., et al
  • Journal Title: Acta Histochem.Cytoc. Volume: (In press)(Not announced)
  • Peer Reviewed
• [Journal Article] Supressioion of choroidal neovascu-larization by single intravitreal injection of APRPG-modified liposomes encapsulating SU5416 (2011)
  • Author(s): Honda M, Asai T, Umemoto T, Araki Y, Oku N, Tanaka M.
  • Journal Title: Arch Ophthalmol Volume: 129 Pages: 317-321
  • Peer Reviewed
• [Journal Article] Quantitative peptidomic analysis by a newly developed one-step direct transfer technology without depletion of major blood proteins : Its potential utility for monitoring of pathophysiological status in pregnancy-induced hypertension (2011)
  • Author(s): Araki Y, Nonaka D, Tajima A, Maruyama M, Nitto T, Ishikawa H, Yoshitake H, Yoshida E, Kuronaka N, Asada K, Yanagida M, Nojima M, Yoshida K, Takamori K, Hashiguchi T, Maruyama I, Lee L-J, Tanaka K
  • Journal Title: Proteomics Volume: 11 Pages: 2727-2737
  • Peer Reviewed
• [Journal Article] Molecular characterization and expression of dipeptidase 3, a testis-specific membrane bound dipeptidase related to an anti-sperm auto-monoclonal antibody epitope (2011)
  • Author(s): Yoshitake H, Yanagida M, Maruyama M, Takamori K, Hasegawa A, Araki Y.
  • Journal Title: J. Reprod. Immunol Volume: 90 Pages: 202-213
  • Peer Reviewed
• [Journal Article] Gene suppression of mouse testis in vivo using small interfering RNA derived from plasmid vectors (2011)
  • Author(s): Takizawa T, Ishikawa T, Kosuge T, Mizuguchi Y, Sato Y, Koji T, Araki Y, Takizawa T.
  • Journal Title: Acta Histochem. Cytoc Volume: (in press)
  • NAID: 130001854310
  • Peer Reviewed
• [Journal Article] Molecular characterization and expression of dipeptidase 3, a testis-specific membrane bound dipeptidase related to an anti-sperm auto-monoclonal antibody epitope (2011)
  • Author(s): Yoshitake H., et al
  • Journal Title: J.Reprod.Immunol. Volume: 90(Not announced) Pages: 202-213
  • Peer Reviewed
• [Journal Article] Possible contribution of circulating blood cells to embryo implantation (2010)
  • Author(s): Fujiwara H, IdetaA, Araki Y, Takao Y, Sato Y, Horie A, Tsunoda N, Aoyagi Y, KonishiI.
  • Journal Title: Indian J. Physiol. Pharmacol Volume: 54, Suppl 1 Pages: 51-60
  • Peer Reviewed
• [Journal Article] Influences of gender and age on relationships between alcohol drinking and atherosclerotic risk factors (2010)
  • Author(s): Wakabayashi I, Araki Y.
  • Journal Title: Alcohol. Clin. Exp. Res Volume: 34
  • Peer Reviewed
• [Journal Article] Molecular expression of Ly6k, a glycosylphosphatidyl-inositol anchored membrane protein on the mouse testicular germ cells (2010)
  • Author(s): Maruyama M, Yoshitake H, Tsukamoto H, Takamori K, Araki Y.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 402 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Postnatal male germ cell expression of the Cre recombinase in Tex101-iCre transgenic mice (2010)
  • Author(s): Lei Z, Lin J, Li X, Li S, Zhou H, Araki Y, Lan Z-J
  • Journal Title: Genesis Volume: 48 Pages: 717-722
  • Peer Reviewed
• [Journal Article] Molecular expression of Ly6k, a glycosylphosphatidyl-inositol anchored membrane protein o the mouse testicular germ cells. (2010)
  • Author(s): Maruyama M., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 402 Pages: 75-81
  • Peer Reviewed
• [Journal Article] Postnatal male germ cell expression of the Cre recombinase in Tex101-iCre transgenic mice. (2010)
  • Author(s): Lei Z., et al.
  • Journal Title: Genesis Volume: 48 Pages: 707-722
  • Peer Reviewed
• [Journal Article] Possible contribution of circulating blood cells to embryo implantation. (2010)
  • Author(s): Fujiwara H., et al.
  • Journal Title: Indian J.Physiol.Pharmacol. Volume: 54,Suppl.1 Pages: 51-60
  • Peer Reviewed
• [Journal Article] Distribution of molecular epitope for Ts4, an anti-sperm auto-monoclonal antibody in the fertilization process (2009)
  • Author(s): Shirai Y, Yoshitake H, Maruyama M, Takamori K, Ogawa H, Hasagawa A, Araki Y.
  • Journal Title: J. Reprod. Dev Volume: 55 Pages: 240-246
  • NAID: 10024942147
  • Peer Reviewed
• [Journal Article] Associations of alcohol drinking with blood pressure and serum lipids in female smokers and nonsmokers (2009)
  • Author(s): Wakabayashi I, Araki Y.
  • Journal Title: Gender Med Volume: 6 Pages: 290-299
  • Peer Reviewed
• [Journal Article] Is the zona pellucida an intrinsic source of signals activating maternal recognition of the developing mammalian embryo (2009)
  • Author(s): Fujiwara H, Araki Y, Toshimori K.
  • Journal Title: J. Reprod. Immunol Volume: 81 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Equatorin : identification and characterization of the epitope of the MN9 antibody in the mouse (2009)
  • Author(s): Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Takamori K, Ogawa H, Araki Y, Miyado K, Toyama Y, Toshimori K.
  • Journal Title: Biol. Reprod Volume: 81 Pages: 889-897
  • Peer Reviewed
• [Journal Article] Immune system cooperatively supports endocrine system-primed embryo implantation. J. Mammal (2009)
  • Author(s): Fujiwara H, Ideta A, Araki Y, Takao Y, Sato Y, Tsunoda N, Aoyagi Y, Konishi I.
  • Journal Title: Ova. Res Volume: 26 Pages: 122-128
  • Peer Reviewed
• [Journal Article] Associations of alcohol drinking with blood pressure and serum lipids in female smokers and nonsmokers (2009)
  • Author(s): Wakabayashi I, Araki Y.
  • Journal Title: Gender Medicine 5 Pages: 220-229
  • Peer Reviewed
• [Journal Article] Distribution of molecular epitope for Ts4, an anti-sperm auto-monoclonal antibody in the fertilization process (2009)
  • Author(s): Shirai Y, et al.
  • Journal Title: Journal of Reproduction and Development 55 Pages: 240-246
  • Peer Reviewed
• [Journal Article] Is the zona pellucida an intrinsic source of signals activating maternal recognition of the developing mammalian embryo? (2009)
  • Author(s): Fujiwara H, et al.
  • Journal Title: Journal of Reproductive Immunology 81 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Equatorin : An acrosomal N,O-sialoglycoprotein involved in sperm-egg fusion (2009)
  • Author(s): Yamatoya K, et al.
  • Journal Title: Biology of Reproduction 81 Pages: 889-897
  • Peer Reviewed
• [Journal Article] Immune system cooperatively supports endocrine system-primed embryo implantation (2009)
  • Author(s): Fujiwara H, et al.
  • Journal Title: Journal of Mammalian Ova Research 26 Pages: 122-128
  • Peer Reviewed
• [Presentation] Circulating immune cells can promote embryo implantation (2011)
  • Author(s): Fujiwara H., Araki Y.
  • Organizer: 31^<st> Annual Meeting of the American Society for Reproductive Immunology
  • Place of Presentation: Salt Lake City, UT, USA
• [Presentation] 抗精子自己抗体Ts4標的分子の受精における作用及び対応抗原の分子性状解析 (2011)
  • Author(s): 吉武洋、柳田光昭、遠藤周一郎、高森建二、長谷川昭子、藤原浩、荒木慶彦
  • Organizer: 第26回日本生殖免疫学会学術集会
  • Place of Presentation: 名古屋市中村区・ウィンクあいち
• [Presentation] 精巣内生殖細胞特異的な発現を示すTEX101ノックアウトマウスの作製と機能解析 (2011)
  • Author(s): 藤原祥高, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市西区・パシフィコ横浜
• [Presentation] Molecular characterization and expression of alpha-N-acetylglucosaminidase, a potential target molecule recognized with an anti-sperm auto-monoclonal antibody, Ts4. (2010)
  • Author(s): Yoshitake H., et al.
  • Organizer: 43th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Milwaukee, WI, USA
  • Year and Date: 2010-08-01
• [Presentation] Molecular expression of Ly6k, a putative glycosylphosphatidylinositol anchored membrane protein on the mouse testicular germ cells. (2010)
  • Author(s): Maruyama M., et al.
  • Organizer: 43th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Milwaukee, WI, USA
  • Year and Date: 2010-07-31
• [Presentation] Molecular expression of Ly6k, a putative glycosylphosphatidylinositol-anchored membrane protein on the mouse testicular germ cells (2010)
  • Author(s): Maruyama M, Yoshitake H, Takamori K, Araki Y.
  • Organizer: 43th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Milwaukee, WI, USA
• [Presentation] Molecular characterization and expression of alpha-N-acetylglucosaminidase, a potential target molecule recognized with an anti-sperm auto-monoclonal antibody, Ts4 (2010)
  • Author(s): Yoshitake H., Yanagida M., Maruyama M., Takamori K., Hasegawa A., Araki Y.
  • Organizer: 43th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Milwaukee, WI, USA
• [Presentation] Germ Cell-Selective Ablation of Fgfr-1 or Fgfr-2 in mice does not affect male fertility (2010)
  • Author(s): Li S., Lin J., Li X., Araki Y., Lan ZJ, Lei Z
  • Organizer: 43th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Milwaukee, WI, USA
• [Presentation] Effects of Ts4, an anti-sperm auto-monoclonal antibody on fertilization in the mouse (2009)
  • Author(s): Yoshitake H, et al.
  • Organizer: 42th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Pittsburgh, PA, USA
  • Year and Date: 2009-07-20
• [Presentation] Effects of Ts4, an anti-sperm auto-monoclonal antibody on fertilization in the mouse (2009)
  • Author(s): Yoshitake H, Maruyama M, Yanagida M, Takamori K, Ogawa H, Hasegawa A, Araki Y.
  • Organizer: 42th Annual Meeting of the Society for the Study of Reproduction
  • Place of Presentation: Pittsburgh, PA, USA Biol. Reprod.
• [Presentation] 抗精子抗体Ts4関連分子Di-petidase 3のマウス精巣における分子性状と発現に関する検討 (2009)
  • Author(s): 吉武洋、丸山真由子、柳田光昭、高森建二、長谷川昭子、荒木慶彦
  • Organizer: 第24回日本生殖免疫学会学術集会
  • Place of Presentation: 東京都新宿区・京王プラザホテルReprod. Immunol. Biol.
• [Book] Immune regulation of human embryo implantation by circulating blood cells. In : The Human Embryo (2011)
  • Author(s): Fujiwara H, Sato Y, Ideta A, Aoyagi Y, Araki Y, Imakawa K, Yamada S
  • Publisher: InTech Open Access Publisher
• [Remarks]
  • URL: http://www.juntendo.ac.jp/english/department/gra/gender_specific_medicine/araki.html
• [Remarks]
  • URL: http://www.juntendo.ac.jp/english/department/gra/gender_specific_medicine/araki.html
• [Remarks]
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/kankyo_igaku/index.html