| Project/Area Number |
21592387
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
HIROTA Kastuhiko 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (60199130)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAKE Youichirou 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (80136093)
NEMOTO Ken 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (10218274)
KAYAMA Shizuo 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (50432761)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | S. intermedius / 細胞溶解毒素 / 胆管上皮細胞 / アポトーシス / Ca^<2+>振動 / NFAT / サイトカイン / カルシニューリン / 感染防御免疫 / サイトカインバランス / Bio-plex測定法 / THP-1 / バランス異常 / IL-10 / S.intermedius / 自己免疫性肝疾患 / 細菌毒素 / 小胞体異常 / gp210 / BiP / ヌクレアポリン / 口腔レンサ球菌 |
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| Research Abstract |
In the present study we analyzed possible relationship between calcium oscillations and each phenomenon in order to find out the molecular mechanisms in ILY-induced bile duct epithelial cells. Intracellular free calcium was analyzed by performing calcium imaging analysis of Fura-2 loaded cells. Increased free calcium was initially detected in the nuclei of ILY-treated cells subsequently followed with markedly increase in ER area. Calcineurin inhibitor cyclosporine A prevented ILY-induced calcineurin/ NFAT1 activation, cell death, and calcium oscillations. ILY in concentration of 10-40 ng/ ml capable of inducing calcium oscillations, activation of calcineurin/ NFAT1 signalling pathway ; inactivation of PI3K/ AKT/ MTOR pathway, and caused ER stress disorder resulted non-apoptotic cell death in HuCCT1 cells.
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