Endodontic therapeutic application of electro-magnetic wave irradiation for refractory periapical periodontitis and periapical biofilm

• Project/Area Number: 21592423
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: YUMOTO Hiromichi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 助教 (60284303)
• Co-Investigator(Kenkyū-buntansha): MATSUO Takashi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (30173800) ; OZAKI Kazumi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (90214121) ; NAKANISHI Tadashi 徳島大学, 病院, 講師 (00217770) ; NAKAE Hideaki 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (30227730)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯内療法学 / 根尖性歯周炎 / 電磁・高周波 / 細菌 / 骨芽細胞 / 遺伝子発現 / 成長因子 / シグナル伝達 / 歯内療法 / マイクロアレイ / オステオポンティン / バイオフィルム

Research Abstract

The Electro-Magnetic Wave Irradiation(EMWI) exhibited great bactericidal activity(> 95. 65%) to kill various oral pathgens. This bactericidal activity by EMWI at 500 kHz(1 sec/ time) increased in an irradiation number-dependent manner. Moreover, EMWI activated MC3T3-E1 cell proliferation. After EMWI(500kHz, 5sec), various growth factors were upregulated. This study suggests that EMWI may contribute to the beneficial effects on bone tissue repair as regenerative therapy and be applicable to refractory periapical periodontitis.

Research Products

• [Journal Article] Bactericidal activity and oral pathogen inactivation by electromagnetic wave irradiation (2012)
  • Author(s): Hiromichi Yumoto, Toshihiko Tominaga, Kouji Hirao, Tomoko Kimura, Kanako Takahashi, Takashi Sumitomo, Naoki Bando, Takashi Matsuo
  • Journal Title: Journal of Applied Microbiology Volume: (In press) Issue: 1 Pages: 181-191
  • DOI: 10.1111/j.1365-2672.2012.05307.x
  • Peer Reviewed
• [Journal Article] Bactericidal activity and oral pathogen inactivation by electromagnetic wave irradiation (2012)
  • Author(s): Hiromichi Yumoto, et al
  • Journal Title: Journal of Applied Microbiology Volume: (In press)
  • Peer Reviewed
• [Journal Article] 電磁波照射の歯内療法への応用. EMAT(Electro-Magnetic Apical Treatment)- (2011)
  • Author(s): 坂東直樹、富永敏彦、湯本浩通、住友孝史、平尾早希、平尾功治、松尾敬志
  • Journal Title: 歯内療法学会雑誌 Volume: Vol.32、No.3 Pages: 184-200
  • NAID: 10029697519
  • Peer Reviewed
• [Journal Article] 電磁波照射の歯内療法への応用-EMAT (Electro-Magnetic Apical Treatment)- (2011)
  • Author(s): 坂東直樹, 他
  • Journal Title: 歯内療法学会雑誌 Volume: 32 Pages: 184-200
  • Peer Reviewed
• [Journal Article] MPC-Polymer Reduces Adherence and Biofilm Formation by Oral Bacteria (2011)
  • Author(s): Katsuhiko Hirota, et al.
  • Journal Title: Journal of Dental Research Volume: (In press)
  • Peer Reviewed
• [Journal Article] Enhanced germicidal effects of pulsed UV-LED irradiation on biofilms (2010)
  • Author(s): Jing Li, et al.
  • Journal Title: Journal of Applied Microbiology Volume: 109 Pages: 2183-2190
  • Peer Reviewed
• [Journal Article] The effects of Electro-Magnetic Wave on Oral Bacterial Viability (2010)
  • Author(s): 湯本浩通, 他
  • Journal Title: 日本細菌学雑誌 65 Pages: 210-210
• [Journal Article] The effects of Electro-Magnetic Wave on Bactetial Viability and Osteoblastic Cell Proliferation (2009)
  • Author(s): TOSHIHIKO TOMINAGA, et al.
  • Journal Title: Journal of Endodontics 35 Pages: 436-436
• [Presentation] Basic Research aimed at Application of Electro-Magnetic Wave Irradiation for Endodontic Treatment (2011)
  • Author(s): Hiromichi Yumoto
  • Organizer: The 13^<th> Joint-Scientific Meeting of Korean Academy of Conservative Dentistry & Japanese Society of Conservative Dentistry
  • Place of Presentation: Kim Koo Museum & Library(Korea, Seoul)
  • Year and Date: 2011-11-11
• [Presentation] Basic Research aimed at Application of Electro-Magnetic Wave Irradiation for Endodontic Treatment (2011)
  • Author(s): Hiromichi Yumoto
  • Organizer: The 13th Joint-Scientific Meeting of KACD & JSCD
  • Place of Presentation: Kim Koo Museum & Libarary (Seoul, Korea)(招待特別講演)
  • Year and Date: 2011-11-11
• [Presentation] 高周波・電磁波照射による骨芽細胞のGrowth Factorの発現・産生誘導 (2011)
  • Author(s): 湯本浩通
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪国際交流センター(大阪市)
  • Year and Date: 2011-10-20
• [Presentation] 高周波・電磁波照射による骨芽細胞のGrowth Factorの発現・産生誘導 (2011)
  • Author(s): 湯本浩通, 他
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大坂国際交流センター(大阪府)
  • Year and Date: 2011-10-20
• [Presentation] A Case Report ; Electro-Magnetic Apical Treatment (2011)
  • Author(s): Takashi Sumitomo, et.al.
  • Organizer: The 9th KAE-JEA Joint Meeting
  • Place of Presentation: 長崎全日空ホテルグラバーヒル(長崎県)
  • Year and Date: 2011-07-30
• [Presentation] 高周波・電磁波照射を歯内療法に応用した症例 (2011)
  • Author(s): 湯本浩通
  • Organizer: 第134回日本歯科保存学会春季学術大会
  • Place of Presentation: 東京ベイ舞浜ホテルクラブリゾート(浦安市)
  • Year and Date: 2011-06-09
• [Presentation] 高周波・電磁波照射を歯内療法に応用した症例 (2011)
  • Author(s): 湯本浩通, 他
  • Organizer: 第134回日本歯科保存学会春季学術大会
  • Place of Presentation: 東京ベイ舞浜ホテルクラブリゾート(千葉県)
  • Year and Date: 2011-06-09
• [Presentation] Electro-Magnetic Apical Treatmentを行った1例 (2010)
  • Author(s): 住友孝史, 他
  • Organizer: 第31回日本歯内療法学会学術大会
  • Place of Presentation: 東京商工会議所
  • Year and Date: 2010-07-25
• [Presentation] Novel Therapeutic Approach of Electro-Magnetic Wave for Infected Root Canal (2010)
  • Author(s): TOSHIHIKO TOMINAGA, et al.
  • Organizer: 88^<th> General Session, & Exhibition of the International Association for Dental Research
  • Place of Presentation: Centre Convencions Internacional Barcelona
  • Year and Date: 2010-07-16
• [Presentation] Inactivation and Bactericidal Activity against Oral Bacteria of Electro-Magnetic Wave (2010)
  • Author(s): Hiromichi Yumoto
  • Organizer: 88^<th> General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: Centre Convencions Internacional Barcelona(Spain, Barcelona)
  • Year and Date: 2010-07-15
• [Presentation] Inactivation and Bactericidal Activity against Oral Bacteria of Electro-Magnetic Wave (2010)
  • Author(s): HIROMICHI YUMOTO, et al.
  • Organizer: 88^<th> General Session, & Exhibition of the International Association for Dental Research
  • Place of Presentation: Centre Convencions Internacional Barcelona
  • Year and Date: 2010-07-15
• [Presentation] 難治性根尖性歯周炎に対する電磁波の応用-EMAT(Electro-Magnetic Apical Treatment)- (2010)
  • Author(s): 坂東直樹, 他
  • Organizer: 第10回日本歯内療法学会西日本支部研修会
  • Place of Presentation: ブリーゼプラザ小ホール
  • Year and Date: 2010-07-04
• [Presentation] The effects of Electro-Magnetic-Wave on Oral Bacterial Viability (2010)
  • Author(s): 湯本浩通
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2010-03-29
• [Presentation] The effects of Electro-Magnetic-Wave on Oral Bacterial Viability (2010)
  • Author(s): 湯本浩通, 他
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-03-29
• [Presentation] Electro-Magnetic Endodontics (2009)
  • Author(s): 湯本浩通, 他
  • Organizer: 徳友会学術セミナー
  • Place of Presentation: 徳島県歯科医師会館
  • Year and Date: 2009-11-15
• [Presentation] 口腔病原菌に対する高周波・電磁波照射の殺菌効果 (2009)
  • Author(s): 湯本浩通
  • Organizer: 第131回日本歯科保存学会秋季学術大会
  • Place of Presentation: 仙台国際センター(仙台市)
  • Year and Date: 2009-10-29
• [Presentation] 口腔病原菌に対する高周波・電磁波照射の殺菌効果 (2009)
  • Author(s): 湯本浩通, 他
  • Organizer: 第131回日本歯科保存学会秋季学術大会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2009-10-29
• [Presentation] The effects of Electro-Magnetic-Wave on bacterial viability and osteoblastic cell proliferation (2009)
  • Author(s): TOSHIHIKO TOMINAGA, et al.
  • Organizer: 2009 Annual Session, American Association of Endodontics
  • Place of Presentation: Gayload Palms Resort and Convention Center(フロリダサ)
  • Year and Date: 2009-04-30
• [Presentation] Electro-Magnetic Pulpectomy (2009)
  • Author(s): TOSHIHIKO TOMINAGA, et al.
  • Organizer: The 15^<th> Scientific Meeting and the 12^<th> Binnial General Meeting of the Asian Pacific Endodontic Confederation
  • Place of Presentation: 都市センターホテル(東京)
  • Year and Date: 2009-04-25
• [Remarks] 口腔病原菌の殺菌に高周波・電磁波照射が有用、Medical Tribune、2010. 5. 6.
• [Remarks] Tissue Regeneration, NHK World News, 2009. 8. 4