Involvement of cytokine network in the pathology of neuropathic pain following peripheral nerve injury

• Project/Area Number: 21600017
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 疼痛学
• Research Institution: Hyogo Medical University
• Principal Investigator: KAMIHARA Masahito 兵庫医科大学, 医学部, 助教 (10441312)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Kimiko 兵庫医科大学, 医学部, 助教 (70418961) ; ARIMURA Yoshinobu 兵庫医科大学, 医学部, 助教 (30419824) ; NAKANO Susumu 兵庫医科大学, 医学部, 助教 (40412019)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 末梢神経損傷 / マイクログリア / アストロサイト / TNF alpha / 脊髄後角 / 神経因性疼痛 / サイトカイン / 神経傷害性疼痛 / TNF受容体

Research Abstract

Tumor necrosis factor(TNF)-alpha is one of a pro-inflammatory cytokines that may play a key role for the induction of neuropathic pain following nerve injury. However, the precise histological expression of TNF alpha and its receptors mRNA in the spinal cord after nerve injury is not fully understood. TNF alpha mRNA showed the transient increase from 18 hour and peaked at 24 hour following SNI. In the spinal cord of naive rats, TNF alpha and TNFR2 showed a low level expression and TNFR1 mRNA signal was detected in neurons and glial cells in the dorsal horn. Twenty-four hours after SNI, TNF alpha mRNA signals increased in the ipsilateral microglia. TNFR1 and TNF R2 mRNA signals increased strikingly in ipsilateral glial cells at 3 days after SNI.

Research Products

• [Journal Article] ペインクリニックにおける慢性疼痛の治療 (2010)
  • Author(s): 村川和重, 他9名
  • Journal Title: Journal of Neurosciences for Pain Research Volume: 12 Pages: 5-10
  • Peer Reviewed
• [Journal Article] Leukotriene synthases and the receptors induced by peripheral nerve injury in the spinal cord contribute to the generation of neuropathic pain. (2010)
  • Author(s): Okubo M, et.al.
  • Journal Title: Glia 58 Pages: 599-610
  • Peer Reviewed
• [Presentation] 末梢神経損傷後の脊髄に一過性に増加するTNF alpha (2011)
  • Author(s): 神原政仁, 小林希実子, 野口光一
  • Organizer: 第87会日本解剖学会近畿支部学術集会
  • Place of Presentation: 西宮
  • Year and Date: 2011-12-03
• [Presentation] 末梢神経損傷後に脊髄で増加するP2Y受容体 (2009)
  • Author(s): 小林希実子, 他2名
  • Organizer: 第47回日本生物物理学会
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] Transient expression of TNF alpha in the rat spinal cord following peripheral nerve iujury (2009)
  • Author(s): Kobayashi K, Yamanaka H, Okubo M, Dai Y, Noguchi K.
  • Organizer: The 39th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-20
• [Presentation] Transient expression of TNF alpha in the rat spinal cord following peripheral nerve injury (2009)
  • Author(s): Kobayashi K., et.al.
  • Organizer: The 39th Annual Meeting of the Society for Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-20
• [Presentation] 末梢神経損傷後の脊髄後角マイクログリアで増加するP2受容体 (2009)
  • Author(s): 小林希実子, 他3名
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] 末梢神経損傷後に脊髄マイクログリアで増加するP2Y受容体 (2009)
  • Author(s): 小林希実子, 他3名
  • Organizer: 第31回日本疼痛学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-17