Metagenomic analysis of microbial population in intercellular fluid of rice and its application for disease control
| Project/Area Number |
21658015
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant pathology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,310,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2009: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | 植物 / 遺伝子 / メタゲノム / 内生菌 / 植物病理学 |
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| Research Abstract |
Microorganisms inhabiting in intercellular spaces of plants may stimulate plant defense systems or promote plant growth, whereas they are likely to benefit from the exudates of plant cells. In this study, we analyzed the structure of overall community of endophytic microorganisms in plants and their disease suppressive activity. Intercellular fluid (IF)was collected from leaf blades and sheaths of rice.(1)Denaturing gradient gel electrophoresis (DGGE)pattern and nucleotide sequence analyses of 16S and 18S_rDNA fragments amplified from IF DNA seemed to be available to understand global microbial ecology in plants.(2)Pseudomonas sp., bcillus sp., Curtobacterium sp. and Acinetobacter sp. were preferentially isolated as endophytic bacteria from rice IF cultivated by organic farming system.(3)Some of those endophytic bacteria exhibited potential activity to suppress rice blast and rice bacterial rot seedling diseases.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Implications of oligomeric forms of POD-1 and POD-2 proteins isolated from cell walls of the biocontrol agent Pythium oligandrum in relation to their ability to induce defense reactions in tomato2011
Author(s)
Takenaka, S., Yamaguchi, K., Masunaka, A., Hase, S., Inoue, T. and Takahashi, H.
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Journal Title
Journal of Plant Physiology
Volume: 168
Pages: 1972-1979
Related Report
Peer Reviewed
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[Journal Article] Microbial community profiles in intercellular fluid of rice2010
Author(s)
Takahashi, H., Sekiguchi, H., Ito, T., Sasahara, M., Hatanaka, N. Ohba, A., Hase, S., Ando, S. and Takenaka, S.
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Journal Title
Journal of General Plant Pathology
Volume: 77
Pages: 121-131
NAID
Related Report
Peer Reviewed
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