Relationship between Biophysical Properties and Microstructure of Actin Stress Fibers

• Project/Area Number: 21680039
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tohoku University
• Principal Investigator: DEGUCHI Shinji 東北大学, 大学院・工学研究科, 准教授 (30379713)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2009: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
• Keywords: ストレスファイバ / バイオメカニクス / メカノセンサー / メカノバイオロジー / 生物物理 / 細胞骨格 / 非筋アクトミオシン / メカノコントローラー / 生物物理学 / 細胞バイオメカニクス / 生体医工学 / ストレスファイバー / 非筋II型ミオシン / メカノセンサ

Research Abstract

Stress fibers play a key role in regulation of cell homeostasis. Here we investigated their biophysical properties and microstructure. Particularly, we developed a method to isolate stress fibers that are intact with regard to structure (confirmed by western blotting and electron microscopy)and function(confirmed by a contractility assay). We then submitted a molecular mechanism that may underlie the cell shortening-induced disassembly of stress fibers.

Research Products

• [Journal Article] Simultaneous contraction and buckling of stress fibers in individual cells (2012)
  • Author(s): Deguchi, S., Matsui, T. S., Sato, M.
  • Journal Title: Journal of Cellular Biochemistry Volume: 113 Pages: 824-832
  • Peer Reviewed
• [Journal Article] The position and size of individual focal adhesions are determined by intracellular stress-dependent positive regulation (2011)
  • Author(s): Deguchi, S., Matsui, T. S., Iio, K.
  • Journal Title: Cytoskeleton Volume: 68 Pages: 639-651
  • Peer Reviewed
• [Journal Article] Cooperative regulation of vocal fold morphology and stress by the cricothyroid and thyroarytenoid muscles (2011)
  • Author(s): Deguchi, S., Y Kawahara, S Takahashi
  • Journal Title: Journal of Voice Volume: 25 Pages: 255-263
  • Peer Reviewed
• [Journal Article] Producing single microbubbles with controlled size using microfiber (2011)
  • Author(s): Deguchi, S., Takahashi, S., Tanimura, S., Hiraki, H.
  • Journal Title: Advances in Bioscience and Biotechnology Volume: 2 Pages: 385-390
  • Peer Reviewed
• [Journal Article] Measuring surface geometry of adherent cells using oblique transillumination (2011)
  • Author(s): Deguchi, S., Bamba, H.
  • Journal Title: Advances in Bioscience and Biotechnology Volume: 2 Pages: 359-363
  • Peer Reviewed
• [Journal Article] Simulation of human phonation with vocal nodules (2011)
  • Author(s): Deguchi, S., Kawahara, Y.
  • Journal Title: American Journal of Computational Mathematics Volume: 1 Pages: 189-201
  • Peer Reviewed
• [Journal Article] Non-muscle myosin II induces disassembly of actin stress fibres independently of myosin light chain dephosphorylation (2011)
  • Author(s): Matsui, T. S., Kaunas, R., Kanzaki, M., Sato, M., Deguchi, S.
  • Journal Title: Interface Focus Volume: 1 Pages: 754-766
  • Peer Reviewed
• [Journal Article] Multiple roles for myosin II in tensional homeostasis under mechanical loading (2011)
  • Author(s): Kaunas, R., Deguchi, S.
  • Journal Title: Cellular and Molecular Bioengineering Volume: 4 Pages: 182-191
  • Peer Reviewed
• [Journal Article] Mechanism of and threshold biomechanical conditions for falsetto voice onset (2011)
  • Author(s): Deguchi, S.
  • Journal Title: PLoS ONE Volume: 6 Pages: 17503-17503
  • Peer Reviewed
• [Journal Article] Sarcomeric model of stretch-induced stress fiber reorganization (2011)
  • Author(s): R. Kaunas, H. Hsu, S. Deguchi
  • Journal Title: Cell Health and Cytoskeleton Volume: 3 Pages: 13-22
  • Peer Reviewed
• [Journal Article] Deguchi, Sarcomeric model of stretch-induced stress fiber reorganization (2011)
  • Author(s): R.Kaunas
  • Journal Title: Cell Health and Cytoskeleton Volume: 3 Pages: 13-22
  • Peer Reviewed
• [Journal Article] Mechanism of and threshold biomechanical conditions for falsetto voice onset (2011)
  • Author(s): S.Deguchi
  • Journal Title: PLos ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Cooperative regulation of vocal fold morphology and stress by the cricothyroid and thyroarytenoid muscles (2011)
  • Author(s): S.Deguchi
  • Journal Title: Journal of Voice Volume: (未定 印刷中)
  • Peer Reviewed
• [Journal Article] Cyclic stretch-induced stress fiber dynamics-Dependence on strain rate, rho kinase and MLCK, Biochemical and Biophysical (2010)
  • Author(s): C. Lee, C. Haase, S. Deguchi, R. Kaunas
  • Journal Title: Research Communications Volume: 401 Pages: 344-349
  • Peer Reviewed
• [Journal Article] Actin stress fibers are at a tipping point between conventional shortening and rapid disassembly at physiological levels of MgATP, Biochemical and Biophysical (2010)
  • Author(s): T. S. Matsui, K. Ito, R. Kaunas, M. Sato, S. Deguchi
  • Journal Title: Research Communications Volume: 395 Pages: 301-306
  • Peer Reviewed
• [Journal Article] Cyclic stretch-induced stress fiber dynamics-dependence on strain rate, rho kinase and MLCK (2010)
  • Author(s): C.Lee
  • Journal Title: Biochemical and Biophysical Research Communi cations Volume: 401 Pages: 344-349
  • Peer Reviewed
• [Journal Article] Actin stress fibers are at a tipping point between conventional shortening and rapid disassembly at physiological levels of MgATP (2010)
  • Author(s): T.S.Matsui, K.Ito, R.Kaunas, M.Sato, S.Deguchi
  • Journal Title: Biochemical and Biophysical Research Communications (未定, 印刷中)
  • Peer Reviewed
• [Journal Article] Biomechanical properties of actin stress fibers of non-motile cells (2009)
  • Author(s): Deguchi, S., Sato, M.
  • Journal Title: Biorheology Volume: 46 Pages: 93-105
  • Peer Reviewed
• [Journal Article] A versatile micro-mechanical tester for actin stress fibers isolated from cells (2009)
  • Author(s): T. S. Matsui, S. Deguchi, N. Sakamoto, T. Ohashi, M. Sato
  • Journal Title: Biorheology Volume: 46 Pages: 401-415
  • Peer Reviewed
• [Journal Article] Measurement and finite element modeling of the force balance in the vertical section of adhering vascular endothelial cells (2009)
  • Author(s): S. Deguchi, Y. Ishimaru, K. Hashimoto, S. Washio, K. Tsujioka
  • Journal Title: Journal of the Mechanical Behavior of Biomedical Materials Volume: 2 Pages: 173-185
  • Peer Reviewed
• [Journal Article] Measurement and finite element modeling of the force balance in the vertical section of adhering vascular endothelial cell (2009)
  • Author(s): S.Deguchi, H.Fukamachi, K.Hashimoto, K.Iio, K.Tsujioka
  • Journal Title: Journal of the Mechanical Behavior of Biomedical Materials 2 Pages: 173-185
  • Peer Reviewed
• [Journal Article] Biomechanical properties of actin stress fiber of non-motile cells (2009)
  • Author(s): S.Deguchi, M.Sato
  • Journal Title: Biorheology 46 Pages: 93-105
  • Peer Reviewed
• [Journal Article] A versatile micro-tensile test system for viscoelastic measurement of isolated actin stress fibers (2009)
  • Author(s): T.S.Matsui, S.Deguchi, N.Sakamoto, T.Ohashi, M.Sato
  • Journal Title: Biorheology 46 Pages: 401-415
  • Peer Reviewed
• [Presentation] Positive and negative feedbacks in cell adhesion regulation and tensional homeostasis (2012)
  • Author(s): Deguchi, S.
  • Organizer: International Symposium on Cellular Mechanobiology
  • Place of Presentation: Kyoto
  • Year and Date: 2012-03-16
• [Presentation] Positive and Negative Feedbacks in Cell Adhesion Regulation and Tensional Homeostasis (2012)
  • Author(s): Deguchi, S.
  • Organizer: International Symposium on Cellular Mechanobiology
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] Biophysical properties of actin stress fibers (2012)
  • Author(s): T.S.Matsui, H.Onodera, Y.Minegishi, A.C.Saito, M.Sato, S.Deguchi
  • Organizer: International Symposium on Cellular Mechanobiology
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] 平滑筋細胞と繊維芽細胞の配向制御法について (2012)
  • Author(s): 齋藤明, 出口真次, 松井翼, 佐藤正明
  • Organizer: 日本機械学会東北支部第47期総会・講演会
  • Place of Presentation: 仙台
  • Year and Date: 2012-03-13
• [Presentation] Tensional homeostasis in a dynamic mechanical environment (2012)
  • Author(s): Kaunas, R., Deguchi, S.
  • Organizer: Multiscale Methods and Validation in Medicine and Biology I : Biomechanics and Mechanobiology
  • Place of Presentation: San Francisco, USA(招待講演)
  • Year and Date: 2012-02-13
• [Presentation] 細胞内張力伝達要素ストレスファイバの形成・維持の分子メカニズム (2012)
  • Author(s): 松井翼, 出口真次, 佐藤正明
  • Organizer: 第24回バイオエンジニアリング講演会
  • Place of Presentation: 大阪
  • Year and Date: 2012-01-07
• [Presentation] Cellular tensional homeostasis regulated by myosin ii (2012)
  • Author(s): Deguchi, S.
  • Organizer: 2nd BMES-SPRBM Inaugural Conference on Cellular and Molecular Bioengineering(30th Scientific Conference of SPRBM)
  • Place of Presentation: San Juan, Puerto Rico
  • Year and Date: 2012-01-05
• [Presentation] Role of stress fibers in cellular tensional homeostasis (2012)
  • Author(s): Deguchi, S., Matsui, T.S., Kaunas, R., Sato, M.
  • Organizer: 2nd BMES-SPRBM Inaugural Conference on Cellular and Molecular Bioengineering
  • Place of Presentation: San Juan, Puerto Rico(招待講演)
  • Year and Date: 2012-01-05
• [Presentation] Adaptation of nonmuscle cells to mechanical environment (2011)
  • Author(s): Deguchi, S., Matsui, T.S., Sato, M.
  • Organizer: International Symposium on Mechanobiology
  • Place of Presentation: Shanghai, China(招待講演)
  • Year and Date: 2011-11-06
• [Presentation] Cellular tensional homeostasis regulated by nonmuscle myosin-II (2011)
  • Author(s): Deguchi, S., Matsui, T.S., Kaunas, R., Sato, M.
  • Organizer: International Symposium on Mechanobiology
  • Place of Presentation: Shanghai, China(招待講演)
  • Year and Date: 2011-11-05
• [Presentation] Nonmuscle myosin II-based regulation of cellular tensional homeostasis (2011)
  • Author(s): Deguchi, S., Matsui, T.S., Kaunas, R., Sato, M.
  • Organizer: Symposium : Developing Mechanobiology : from fundamentals to medical applications in The 49th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: 姫路(招待講演)
  • Year and Date: 2011-09-16
• [Presentation] Celluar adaptation to physical environment (2011)
  • Author(s): Deguchi, S., Matsui, T.S., Sato, M.
  • Organizer: 生物物理学会・東北支部講演会
  • Place of Presentation: 仙台
  • Year and Date: 2011-06-17
• [Presentation] アクチンストレスファイバが張力依存的に脱重合するメカニズムの考察 (2011)
  • Author(s): 出口真次
  • Organizer: 第23回バイオエンジニアリング講演会
  • Place of Presentation: 熊本、熊本大学
  • Year and Date: 2011-01-09
• [Presentation] アクチンストレスファイバは筋原繊維のように弛緩するか? (2011)
  • Author(s): 松井翼
  • Organizer: 第23回バイオエンジニアリング講演会
  • Place of Presentation: 熊本、熊本大学
  • Year and Date: 2011-01-09
• [Presentation] Multiple roles for myosin II in tensional homeostasis-Experimentand theory (2011)
  • Author(s): R.Kaunas
  • Organizer: BMES-SPRBM Inaugural Conference on Cellular and Molecular Bioengineering, 29th Scientific Conference of SPRBM
  • Place of Presentation: アメリカ、Miami Beach
  • Year and Date: 2011-01-06
• [Presentation] Nonmuscle actomyosin bundles have contractile properties similar to but distinct from those of striated muscle (2010)
  • Author(s): T.S.Matsui
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台、東北大学
  • Year and Date: 2010-09-21
• [Presentation] Cellular adaptation to physical environment based on nonmuscle actomyosin contractility (2010)
  • Author(s): S.Deguchi
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台、東北大学
  • Year and Date: 2010-09-20
• [Presentation] アクチンストレスファイバのねじり収縮 (2010)
  • Author(s): 松井翼
  • Organizer: 日本機械学会2010年度年次大会
  • Place of Presentation: 名古屋、名古屋工業大学
  • Year and Date: 2010-09-08
• [Presentation] 再生系細胞に存在する張力ホメオスタシスの幾つかの証拠 (2010)
  • Author(s): 出口真次
  • Organizer: 日本機械学会2010年度年次大会
  • Place of Presentation: 名古屋、名古屋工業大学
  • Year and Date: 2010-09-07
• [Presentation] Theoretical and experimental study of the mechanism of phonation onset (2010)
  • Author(s): S.Deguchi
  • Organizer: 6th World Congress of Biomechanics
  • Place of Presentation: シンガポール、Suntec Convention Centre
  • Year and Date: 2010-08-05
• [Presentation] Cellular adaptation I : Contractile properties of actin stress fibers (2010)
  • Author(s): T.S.Matsui
  • Organizer: 6th World Congress of Biomechanics
  • Place of Presentation: シンガポール、Suntec Convention Centre
  • Year and Date: 2010-08-03
• [Presentation] Cellular adaptation II : Tensional homeostasis based on nonmuscle actomyosin contractility (2010)
  • Author(s): S.Deguchi
  • Organizer: 6th World Congress of Biomechanics
  • Place of Presentation: シンガポール、Suntec Convention Centre
  • Year and Date: 2010-08-03
• [Presentation] A dynamic model of actin stress fiber network adaptation to cyclic stretching (2010)
  • Author(s): R.Kaunas
  • Organizer: 6th World Congress of Biomechanics
  • Place of Presentation: シンガポール、Suntec Convention Centre
  • Year and Date: 2010-08-02
• [Presentation] Proposed mechanism of cellular adaptation to mechanical environment (2010)
  • Author(s): S.Deguchi
  • Organizer: 第49回日本生体医工学会
  • Place of Presentation: 大阪、大阪国際交流センター
  • Year and Date: 2010-06-25
• [Presentation] アクチンストレスファイバの動態調節の分子機構とメカニクス (2010)
  • Author(s): 出口真次, 松井翼
  • Organizer: 第22回バイオエンジニアリング講演会
  • Place of Presentation: 岡山
  • Year and Date: 2010-01-10
• [Presentation] アクチンの取り込みを伴わないストレスファイバの可逆的収縮 (2010)
  • Author(s): 松井翼, 出口真次
  • Organizer: 第22回バイオエンジニアリング講演会
  • Place of Presentation: 岡山
  • Year and Date: 2010-01-10
• [Presentation] アクチンストレスファイバの収縮と構造の関係について (2010)
  • Author(s): 小野寺瞳, 松井翼, 出口真次
  • Organizer: 第22回バイオエンジニアリング講演会
  • Place of Presentation: 岡山
  • Year and Date: 2010-01-10
• [Presentation] Biomechanical behaviors of actin stress fibers of non-motile cells (2009)
  • Author(s): S.Deguchi, T.S.Matsui, M.Sato
  • Organizer: The Third Switzerland-Japan Workshop on Biomechanics
  • Place of Presentation: Engelberg, Switzerland
  • Year and Date: 2009-09-02
• [Presentation] In vitro measurement of viscoelastic properties of actin bundles using micro-tensile tester with visual feedback control (2009)
  • Author(s): T.Matsui, S.Deguchi, N.Sakamoto, T.Ohashi, M.Sato
  • Organizer: 4th Asian Pacific Conference on Biomechanics
  • Place of Presentation: Christchurch, New Zealand
  • Year and Date: 2009-04-16
• [Book] バイオメカニクス最前線 (2012)
  • Author(s): 出口真次
  • Publisher: 共立出版(印刷中)
• [Book] バイオメカニクス最前線 (2012)
  • Author(s): 出口真次, 他(共著)
  • Publisher: 共立出版(印刷中)
• [Book] Chapter 9. Nuclear mechanics and mechanotransduction. In"Cellular Mechanotransduction : Diverse Perspectives from Molecules to Tissue" (2010)
  • Author(s): Deguchi, S., Sato, M.
  • Publisher: Cambridge University Press
• [Book] Cellular Mechanotransduction : Diverse Perspectives from Molecules to Tissues (2010)
  • Author(s): S.Deguchi, M.Sato
  • Publisher: Cambridge University Press
• [Remarks]
  • URL: http://www.biomech.mech.tohoku.ac.jp/satolab/deguchi.html
• [Remarks]
  • URL: http://db.tohoku.ac.jp/whois/detail/87bca4b801774ad6e093d0079e81b956.html