| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Research Abstract |
We have developed a combined high-speed atomic force microscopy(AFM) with fluorescence microscopy towards simultaneous observation of single-molecule conformational change of protein and chemical reaction such as ATP binding process. For a stand-alone-type high-speed AFM which can be mounted on an optical microscope, miniaturization of AFM head was carried out and the optical tracking system was developed. Using this system, we succeeded in observing GroEL at a frame rate of 3 frames/s. Further, we confirmed that it enables us to simultaneously obtain AFM and fluorescent images at same area. We also developed a wide-range scanner for fast imaging of bacterial cells and applied it to observation of bacteriolysis reaction of Bacillus subtilis induced by lysozyme.
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