Development of CLIP-RNAi method
Project/Area Number |
21686078
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Biofunction/Bioprocess
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Research Institution | Okayama University |
Principal Investigator |
OHTSUKI Takashi 岡山大学, 大学院・自然科学研究科, 教授 (80321735)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥26,910,000 (Direct Cost: ¥20,700,000、Indirect Cost: ¥6,210,000)
Fiscal Year 2011: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2010: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2009: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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Keywords | RNAi / 光誘導 / shRNA / siRNA / CLIP-RNAi / 遺伝子発現抑制 / RNA結合蛋白質 / 細胞膜透過性ペプチド |
Research Abstract |
RNAi is currently an important experimental tool for basic research into gene function. RNAi also has many potential uses in various biotechnological and therapeutic applications. We recently developed photoinducible RNAi method, termed CLIP-RNAi. In this study, we improved the photoresponsive RNA carrier for CLIP-RNAi. We demonstrate the use of CLIP-RNAi with several fluorescent dyes that can be excited at different wavelengths. We used CLIP-RNAi method in in vitro and in vivo applications.
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] Carrier PNA for shRNA delivery into cells2009
Author(s)
Kitamatsu, M., Kubo, T., Matsuzaki, R., Endoh, T., Ohtsuki, T. and Sisido, M
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Journal Title
Bioorganic & Medicinal Chemistry Letters
Volume: Vol.19
Pages: 3410-3413
NAID
Related Report
Peer Reviewed
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