Analysis for the mechanism of petal growth during flower opening and control for the cut flower opening in post-harvest conditions

• Project/Area Number: 21688002
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Horticulture/Landscape architecture
• Research Institution: Chubu University
• Principal Investigator: YAMADA Kunio 中部大学, 応用生物学部, 准教授 (30345871)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000); Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2009: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
• Keywords: 園芸学 / バラ / ポストハーベスト / 細胞壁 / エクスパンシン / XTH / トルコギキョウ / ポストハーベス

Research Abstract

The aims of this project are to clarify the mechanism of petal growth during flower opening, and to control flower opening of cut roses in post-harvest conditions. I found that the Expansin and XTH play important roles for flower opening of cut roses by real-time PCR analysis and western blot analysis. And XG9 was analyzed as an inhibitor for XTH enzyme activity. XG9 promoted the flower opening of cut roses at high concentration, and in contrast, XG9 inhibited it at low concentration. Furthermore, it became clear that the petal of cut rose buds grew for only a few hours from the initiation of light period, and it hardly grew during the rest periods.

Research Products

• [Journal Article] Structural characteristics of SLF/SFB-genes in apple cultivars with similar S-RNase sequences (2012)
  • Author(s): Hayashi, H., Yamada, K., Shiratake, K. and Matsumoto, S.
  • Journal Title: Acta Horticulturae Volume: 929 Pages: 267-274
  • Peer Reviewed
• [Journal Article] 栽培バラに四季咲き性をもたらしたトランスポゾン:バラゲノム内のコピー数と分布 (2011)
  • Author(s): 河村耕史、岩田光、山田邦夫、松本省吾、福井博一、JEAUFFRE Julien、THOUROUDE Tatiana、HIBRAND-SAINT OYANT Laurence、FOUCHER Fabrice
  • Journal Title: 園芸学研究 Volume: 10(別2) Pages: 223-223
• [Journal Article] Role of expansin and XTH during rose flower opening (2010)
  • Author(s): Ochiai, M., Takagaki, N., Takahashi, R., Fujitani, C. and Yamada, K.
  • Journal Title: Acta Horticulturae Volume: 870 Pages: 273-278
  • Peer Reviewed
• [Journal Article] Effect of plant hormones on invertase activity and on petal growth (2010)
  • Author(s): Horibe, T., Ito, M. and Yamada, K.
  • Journal Title: Acta Horticulturae Volume: 870 Pages: 279-283
  • Peer Reviewed
• [Journal Article] Changes in subcellular concentrations of soluble carbohydrates of petals during flower opening in rose determined by nonaqueous fractionation method combined with infiltration-centrifuged method (2009)
  • Author(s): Yamada, K., Norikoshi, R., Suzuki, K., Imanishi, H. and Ichimura, K.
  • Journal Title: Planta Volume: 230 Pages: 1115-1127
  • Peer Reviewed
• [Journal Article] Cell wall extensibility and effect of cell wall-loosening proteins during rose flower opening (2009)
  • Author(s): Yamada, K., Takahashi, R., Fujitani, C., Mishima, K., Yoshida, M., Joyce, D. C. and Yamaki, S.
  • Journal Title: J. Japan Soc. Hort. Sci Volume: 78 Pages: 242-251
  • NAID: 10025926916
  • Peer Reviewed
• [Journal Article] Cell wall extensibility and effect of cell wall-loosening proteins during rose flower opening (2009)
  • Author(s): Yamada, K., Takahashi, R., Fujitani, C., Mishima, K., Yoshida, M., Joyce, D.C., Yamaki, S.
  • Journal Title: J.Japan Soc.Hort.Sci. Volume: 78 Pages: 242-251
  • NAID: 10025926916
  • Peer Reviewed
• [Journal Article] Cell division and expansion growth during rose petal development (2009)
  • Author(s): Yamada, K., Norikoshi, R., Suzuki, K., Nishijima, T., Imanishi, H., Ichimura, K.
  • Journal Title: J.Japan Soc.Hort.Sci. Volume: 78 Pages: 356-362
  • NAID: 10025927418
  • Peer Reviewed
• [Journal Article] Changes in subcellular concentrations of soluble carbohydrates of petals during flower opening in rose determined by nonaqueous fractionation method combined with infiltration-centrifuged method. (2009)
  • Author(s): Yamada, K., Norikoshi, R., Suzuki, K., Imanishi, H., Ichimura, K.
  • Journal Title: Planta Volume: 230 Pages: 1115-1127
  • Peer Reviewed
• [Journal Article] Cell division and expansion growth during rose petal development (2008)
  • Author(s): Yamada, K., Norikoshi, R., Suzuki, K., Nishijima, T., Imanishi, H. and Ichimura, K.
  • Journal Title: J. Japan Soc. Hort. Sci Volume: 78 Pages: 356-362
  • NAID: 10025927418
  • Peer Reviewed
• [Presentation] The rose genome sequence initiative (2012)
  • Author(s): Foucher, F., Sakr, S., Bendahmane, M., Smulders, M., Debener, T., Riek, J. D., Torres, A. M., Amaya, I., Millan, T., Zamir, D., Sargent, D., Nybom, H., Atanassov, A., Hokanson, S. C., Byrne, D., Sosinski, B., Main, D., Bruneau, A., Rees, J., Matsumoto, S.
  • Organizer: Plant & Animal Genome XX
  • Place of Presentation: San Diego, CA, USA
• [Presentation] 栽培バラに四季咲き性をもたらしたトランスポゾン:バラゲノム内のコピー数と分布 (2011)
  • Author(s): 河村耕史、岩田光、山田邦夫、松本省吾、福井博一、Jeauffre, J.、Thouroude, T.
  • Organizer: 園芸学会平成23年度秋季大会
  • Place of Presentation: 岡山大学
• [Presentation] Role of expansin and XTH during flower opening in lisianthus(Eustoma grandiflorum) (2010)
  • Author(s): Ochiai, M., Matsumoto, ., Shiratake, K., Maesaka, M. and Yamada, K.
  • Organizer: 28^<th> International Horticultural Congress
  • Place of Presentation: リスボン
• [Presentation] Regulation of rose petal growth by cell-elongation inhibitors (2010)
  • Author(s): Takagaki, N., Matsumoto, S., Shiratake, K., Maesaka, M. and Yamada, K.
  • Organizer: 28^<th> International Horticultural Congress
  • Place of Presentation: リスボン
• [Presentation] Role of expansin and XTH during flower opening in lisianthus (Eustoma grandiflorum) (2010)
  • Author(s): Masaki Ochiai, Shogo Matsumoto, Katsuhiro Shiratake, Masahiro Maesaka, Kunio Yamada
  • Organizer: 28^<th> International Horticultural Congress
  • Place of Presentation: リスボン、ポルトガル
• [Presentation] Regulation of rose petal growth by cell-elongation inhibitors (2010)
  • Author(s): Naho Takagaki, Shogo Matsumoto, Katsuhiro Shiratake, Masahiro Maesaka, Kunio Yamada
  • Organizer: 28^<th> International Horticultural Congress
  • Place of Presentation: リスボン、ポルトガル
• [Presentation] バラPHANTASTICAオーソログ(ROPHAN)の構造・機能解析 (2010)
  • Author(s): 林裕作, 河村耕史, 山田邦夫, 白武勝裕, 松本省吾
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
• [Presentation] トルコギキョウ花弁におけるエクスパンシンおよびエンド型キシログルカン転移酵素/加水分解酵素(XTH)の解析 (2009)
  • Author(s): 落合正樹、松本省吾、白武勝裕、山田邦夫
  • Organizer: 園芸学会平成21年度秋季大会
  • Place of Presentation: 秋田大学
• [Presentation] 細胞伸長阻害剤による花弁成長制御 (2009)
  • Author(s): 高垣奈保、松本省吾、白武勝裕、山田邦夫
  • Organizer: 園芸学会平成21年度秋季大会
  • Place of Presentation: 秋田大学
• [Presentation] Role of expansin and XTH duirng rose flower opening (2009)
  • Author(s): Ochiai, M., Takagaki, N., Maesaka, M. and Yamada, K.
  • Organizer: 5th International Symposium on Rose Research and Cultivation
  • Place of Presentation: 岐阜市
• [Presentation] Role of expansin and XTH duirng rose flower opening (2009)
  • Author(s): Ochiai, M., Takagaki, N., Maesaka, M., Yamada, K.
  • Organizer: 5th International Symposium on Rose Research and Cultivation
  • Place of Presentation: 長良川国際会議場、花フェスタ記念公園(岐阜市)