|Budget Amount *help
¥36,270,000 (Direct Cost: ¥27,900,000、Indirect Cost: ¥8,370,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
In this study, I tried to develop new technology to reanimate for extinct or endangered species. First, I had improved the basic nuclear transfer techniques, such as collection of intact donor nuclei from dead body, period of oocyte activation, and HDACi treatment. Second, mouse dried skin, preserved at room temperature for more than 1 year were used as the model of real animal. Although cloned blastocysts were obtained from preserved skin nucleus, those embryos failed to develop beyond blastocyst after embryo transfer. Third, I tried to repeat the nuclear transfer from somatic cell of cloned mouse to examine the possibility of infinite cloning. As the results, so far I could generate over 700 cloned mice from only one donor mouse by 30 times repeating of nuclear transfer.