Molecular mechanisms underlying the release dynamics of synaptic vesicles
| Project/Area Number |
21700417
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Neurochemistry/Neuropharmacology
|
|---|
| Research Institution | National Institute for Physiological Sciences |
|---|
Principal Investigator |
SATAKE Shin'Ichiro National Institute for Physiological Sciences, 生体情報研究系, 助教 (30360340)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | ラット / 小脳 / 分子層介在ニューロン / 顆粒細胞 / ペアパルス増強 / シナプス前性制御 / スライスパッチクランプ法 / 介在ニューロン / 興奮性シナプス後電流 / 減衰時定数 / Gタンパク質共役型受容体 / シナプス前抑制 / 拡散 / グルタミン酸輸送体 |
|---|
| Research Abstract |
Using the whole-cell patch clamp recording in rat cerebellar slices, we found different forms of paired-pulse facilitation (PPF) at the excitatory synaptic transmission from granule cells (GCs) to molecular-layer interneurons (INs). Paired-pulse activation of GC ascending axons at short intervals (30-100 ms) caused a significant facilitation not only in the peak amplitude (PPFamp) but also in the decay-time constant (PPFdecay) of the excitatory postsynaptic currents (EPSCs) recorded from INs. The results of pharmacological tests, kinetics analyses and computer simulations suggest that the mechanisms underlying the respective types of PPF were different. PPFamp was elicited by dual increase in the probability of the vesicular release and in the multiplicity of released vesicles. On the other hand, PPFdecay was caused by extrasynaptic spillover of the GC transmitter glutamate and following their intersynaptic pooling.
|
Report
(3 results)
Research Products
(14 results)
