Development of regenerative medicine of glaucoma using iPS cells

• Project/Area Number: 21700423
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Keio University
• Principal Investigator: YOSHIDA Tetsu Keio University, 医学部, 助教 (00365438)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 緑内障 / 網膜神経節細胞 / 幹細胞 / iPS細胞 / 幹細胞生物学 / 再生 / 修復

Research Abstract

To develop the regenerative medicine of glaucoma, I tried to introduce iPS cell into retinal ganglion cell and Muller cell, which is an adult stem cell in retina. At the same time, identivication of the marker genes of Muller cell were also taken place. First, a recombinant BAC clone, which has a red fluorescent protein DsRed gene at the position of a retinal progenitor cell marker Rx gene, was constructed. And, I found a gene called Epiplakin was a marker gene of Muller cell.

Research Products

• [Journal Article] Retinal ganglion cell loss in superoxide dismutase 1-deficiency. (2011)
  • Author(s): Kenya Y, Ozawa Y, Yoshida T, Kurihara T, Hirasawa M, Ozeki N, Shiba D, Noda K, Ishida S, Tsubota K.
  • Journal Title: Invest.Ophthal.Vis.Sci. in press
  • Peer Reviewed
• [Journal Article] Epiplakinl marks the cholangiocyte lineage in normal liver and adult progenitors in injured liver. (2011)
  • Author(s): Matsuo A, Yoshida T, Yasukawa T, Miki R, Ishikawa K, Fujiwara S, Kume K, Kume S.
  • Journal Title: Gene Expr Patterns. 11 Pages: 255-262
  • Peer Reviewed
• [Journal Article] Retinal ganglion cell loss in superoxide dismutase 1-deficiency (2011)
  • Author(s): Yuki K., et al.
  • Journal Title: Investigative of Ophthalogy and Visual Science Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Epiplakin1 marks the cholangiocyte lineage in normal liver and adult progenitors in injured liver. (2011)
  • Author(s): Matsuo A., et al.
  • Journal Title: Gene Expression Patterns Volume: 11 Pages: 255-262
  • Peer Reviewed
• [Journal Article] Analysis on the expression and function of BRINP family genes during neural differentiation of mouse embryonic stem cell-derived neural stem cells. (2010)
  • Author(s): Terashima M, Kobayashi M, Motomiya M, Inoue N, Yoshida T, Okano H, Iwasaki N, Minami A, Matsuoka I.
  • Journal Title: J.Neurosci.Res. 88 Pages: 1387-1393
  • Peer Reviewed
• [Journal Article] Expression of Epiplakinl in the developing and adult mouse retina. (2010)
  • Author(s): Yoshida T, Guo X, Namekata K, Mitamura Y, Kume S, Harada T.
  • Journal Title: Jpn J.Ophthalmol. 54 Pages: 85-88
  • Peer Reviewed
• [Journal Article] Analysis on the expression and function of BRINP family genes during neural differentiation of mouse embryonic stem cell-derived neural stem cells (2010)
  • Author(s): Terashima M., et al.
  • Journal Title: Journal of Neuroscience Research Volume: 88 Pages: 1387-1393
  • Peer Reviewed
• [Journal Article] Expression of Epiplakin1 in the developing and adult mouse retina (2010)
  • Author(s): Yoshida T., et al.
  • Journal Title: Japanese Journal of Ophthalmology Volume: 54 Pages: 85-88
  • NAID: 10027203601
  • Peer Reviewed
• [Journal Article] Expression of Epiplakinl in the developing and adult mouse retina (2010)
  • Author(s): Yoshida T., et al.
  • Journal Title: Japanese Journal of Ophthalmology 54 Pages: 85-88
  • Peer Reviewed
• [Journal Article] Analysis of gene expressions of ES-derived Pdx1-expressing cells : Implications of genes involved in pancreas differentiation. (2009)
  • Author(s): Yoshida T, Murata K, Shiraki N, Kume K, Kume S.
  • Journal Title: Dev.Growth Differ. 51 Pages: 463-472
  • Peer Reviewed
• [Presentation] ヘルパー依存型アデノウイルスを用いたヒトiPS細胞の遺伝子変異治療 (2011)
  • Author(s): 吉田哲、小泉春菜、結城賢弥、平林由香、鈴木啓一郎、三谷幸之介、小林哲郎、大山学、天谷雅行、岡田洋平、赤松和土、坪田一男、榛村重人、小沢洋子、岡野栄之
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 京王プラザホテル(東京)
• [Presentation] ヘルパー依存型アデノウイルスを用いたヒトiPS細胞の遺伝子変異治療 (2011)
  • Author(s): 吉田哲
  • Organizer: 日本再生医療学会
  • Place of Presentation: 京王プラザホテル(東京)
• [Presentation] A gene therapy for a gene mutation in human iPS cellusing helper-dependent adenoviral vector. (2010)
  • Author(s): Yoshida, T., Koizumi, H., Yuki, Y., Kubota, S., Hirabayashi, Y., Suzuki, K. Mitani, K., Kobayashi, T., Ohyama M., Amagai, M., Okada, Y., Akamatsu, W., Tsubota, K., Shimmra, S., Ozawa, Y., Okano, H.
  • Organizer: The 16^<th> International Conference of the International Society of Differentiation, From Stem Cells to Organisms.
  • Place of Presentation: Nara-ken New Public Hall, Nara, Japan.
• [Presentation] ヘルパー依存型アデノウイルスベクターを用いたヒトiPS細胞の遺伝子変異治療の試みiPS・ES・体性幹細胞Form 2010 (2010)
  • Author(s): 吉田哲
  • Organizer: 基礎研究から臨床応用・創薬研究へ
  • Place of Presentation: 秋葉原UDX GALLERY(東京)
• [Presentation] Purification of iPS cells derived from aretinitis pigmentosa patient from feeder cells. (2010)
  • Author(s): T.Yoshida, H.Koizumi, K.Yuki, K.Tsubota, H.Okano, Y.Ozawa.
  • Organizer: ARVO 2010, The Future of Eye and Vision Research.
  • Place of Presentation: Fort Lauderdale, Florida, USA.
• [Presentation] A gene therapy for a patient with a familial neurodegenerative disease using iPS cell. (2010)
  • Author(s): Tetsu Yoshida, Haruna Koizumi, Kenya Yuki, Keiichiro Suzuki, Kohnosuke Mitani, Kazuo Tsubota, ShigetoShimmra, Yoko Ozawa, Hideyuki Okano.
  • Organizer: Global COE Program Symposium 2010, Education and Research for Sterm Cell Medicine.
  • Place of Presentation: Center for Integrated Medical Research, Keio University, Tokyo, Japan.
• [Presentation] Expression of Epiplakinl in the Developing and Adult Mouse Retina Retina (2010)
  • Author(s): Yoshida T, Kume S, Harada T, Tsubota K, Ozawa Y.
  • Organizer: Retina : Neural Stem Cells and Photoreceptor Degeneration.
  • Place of Presentation: Okinawa Institute of Science and Technology, Okinawa, Japan.
• [Presentation] A gene therapy for a gene mutation in human iPS cell using helper-dependent adenoviral vector (2010)
  • Author(s): Yoshida T., et al.
  • Organizer: The 16^<th> International Conference of the International Society of Differentiation
  • Place of Presentation: 奈良県新公会堂(奈良)
• [Presentation] ヘルパー依存型アデノウイルスを用いたヒトiPS細胞の遺伝子変異治療の試み (2010)
  • Author(s): 吉田哲
  • Organizer: iPS・ES・体性幹細胞Forum 2010～基礎研究から臨床応用・創薬研究へ～
  • Place of Presentation: 秋葉原UDX GALLERY(東京)
• [Presentation] Purification of iPS cells derived from a retinitis pigmentosa patient from feeder cells. (2010)
  • Author(s): Yoshida T., et al.
  • Organizer: The Association for Research in Vision and Ophthalmology (ARVO) 2010
  • Place of Presentation: Fort Lauderdale, Florida, USA
• [Presentation] Epiplakinlの発現パターンの解析 (2009)
  • Author(s): 吉田哲、白木伸明、馬場秀夫、後藤瑞希、藤原作平、粂和彦、粂昭苑
  • Organizer: 第42回日本発生生物学会
  • Place of Presentation: トキメッセ(新潟)
• [Presentation] Expression of Epiplakinl in the developing and adult mouse retina (2009)
  • Author(s): Yoshida T., et al.
  • Organizer: OIST Workshop, Retina : Neural Stem Cell and Photoreceptor Degeneration
  • Place of Presentation: Okinawa Institute of Science and Technology
• [Remarks] ホームページ等 なし