Facile and rapid detection of toxic heavy metal ions using double-stranded DNA-gold nanoparticle conjugates containing mismatch base pair
| Project/Area Number |
21700495
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KANAYAMA Naoki University of Tsukuba, 大学院・数理物質科学研究科, 講師 (80377811)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | DNA / 金ナノ粒子 / 重金属イオン / ミスマッチ塩基対 / 非架橋凝集 |
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| Research Abstract |
Colorimetric detection of mercury ions (Hg^<2+>) with the naked eye was accomplished by a combination of non-crosslinking aggregation of double-stranded DNA-carrying gold nanoparticles (dsDNA-GNP) and complex formation of thymine-Hg^<2+>-thymine (T-Hg^<2+>-T). The dsDNA-GNP having a T-T mismatch site located near the distal end aggregated spontaneously within 1 min in the presence of Hg(ClO_4)_2 at a sub-micromolar concentration in aqueous media at room temperature, resulting in the distinct color change of the particle's dispersion from red to colorless. This unique colloidal behavior was presumably due to the decrease in the entropic repulsion between the particles induced by the T-Hg^<2+>-T complex formation at the interface between the dsDNA layer and the dispersal medium.
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Report
(3 results)
Research Products
(16 results)
