The combination therapy of mild-hyperthermia with therapeutic ultrasound and dendritic cell-based immunotherapy

• Project/Area Number: 21700511
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Medical systems
• Research Institution: Teikyo University
• Principal Investigator: SUZUKI Ryo Teikyo University, 薬学部, 講師 (90384784)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 超音波 / リポソーム / がん温熱療法 / 樹状細胞 / がん免疫療法 / 低侵襲

Research Abstract

Ultrasound (US) exposure to tumor tissue which was intratumorally injected nanobubbles (Bubble liposomes(BLs)), could induce anti-tumor effect by heating and shock waves derived from disruption of BLs. In addition, we found that strong tumor-specific immunity was induced by intratumoral injection of dendritic cells (DCs) after US therapy with BLs. The combination of US therapy with nanobubbles and DC-based immunotherapy efficiently suppressed tumor growth. Therefore, this might be a useful strategy for cancer therapy.

Research Products

• [Journal Article] Progress in the development of ultrasound-mediated gene delivery systems utilizing nano- and microbubbles. (2011)
  • Author(s): Suzuki R, Oda Y, Utoguchi N, Maruyama K.
  • Journal Title: J Control Release. 149 Pages: 36-41
  • Peer Reviewed
• [Journal Article] Progress in the development of ultrasound-mediated gene delivery systems utilizing nano- and microbubbles (2011)
  • Author(s): Ryo Suzuki, Yusuke Oda, Naoki Utoguchi, Kazuo Maruyama
  • Journal Title: J Control. Release Volume: 149 Pages: 36-41
  • Peer Reviewed
• [Journal Article] Synergistic effect of ultrasound and antibiotics against Chlamydia trachomatis-infected human epithelial cells in vitro (2011)
  • Author(s): Yurika Ikeda-Dantsuji, Loreto B.Feril Jr., Katsuro Tachibana, Koichi Ogawa, Hitomi Endo, Yoshimi Harada, Ryo Suzuki, Kazuo Maruyama
  • Journal Title: Ultrasonics Sonochemistry Volume: 18 Pages: 425-430
  • Peer Reviewed
• [Journal Article] Synergistic effect of ultrasound and antibiotics against Chlamydia trachomatis-infected human epithelial cells in vitro. (2010)
  • Author(s): Ikeda-Dantsuji Y, Feril LB Jr, Tachibana K, Ogawa K, Endo H, Harada Y, Suzuki R, Maruyama K.
  • Journal Title: Ultrason Sonochem. 18(1) Pages: 425-430
• [Journal Article] Development of an ultrasound-responsive and mannose-modified gene carrier for DNA vaccine therapy. (2010)
  • Author(s): Un K, Kawakami S, Suzuki R, Maruyama K, Yamashita F, Hashida M.
  • Journal Title: Biomaterials. 31 Pages: 7813-726
  • Peer Reviewed
• [Journal Article] Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice. (2010)
  • Author(s): Okazaki F, Matsunaga N, Okazaki H, Utoguchi N, Suzuki R, Maruyama K, Koyanagi S, Ohdo S.
  • Journal Title: Cancer Res. 70(15) Pages: 6238-6246
  • Peer Reviewed
• [Journal Article] Enhanced transfection efficiency into macrophages and dendritic cells by a combination method using mannosylated lipoplexes and bubble liposomes with ultrasound exposure. (2010)
  • Author(s): Un K, Kawakami S, Suzuki R, Maruyama K, Yamashita F, Hashida M.
  • Journal Title: Hum Gene Ther. 21 Pages: 65-74
• [Journal Article] Development of an ultrasound-responsive and mannose-modified gene carrier for DNA vaccine therapy (2010)
  • Author(s): Keita Un, Shigeru Kawakami, Ryo Suzuki, Kazuo Maruyama, Fumiyoshi Yamashita, Mitsuru Hashida
  • Journal Title: Biomaterials Volume: 31 Pages: 7813-7826
  • Peer Reviewed
• [Journal Article] Cancer gene therapy by IL-12 gene delivery using liposomal bubbles and tumoral ultrasound exposure (2010)
  • Author(s): Suzuki R, Namai E, Oda Y, Nishiie N, Otake S, Koshima R, Hirata K, Taira Y, Utoguchi N, Negishi Y, Nakagawa S, Maruyama K
  • Journal Title: J.Control.Release 142 Pages: 245-250
  • Peer Reviewed
• [Journal Article] A novel strategy utilizing ultrasound for antigen delivery in dendritic cell-based cancer immunotherapy. (2009)
  • Author(s): Suzuki R, Oda Y, Utoguchi N, Namai E, Taira Y, Okada N, Kadowaki N, Kodama T, Tachibana K, Maruyama K.
  • Journal Title: J Control Release. 133 Pages: 198-205
• [Journal Article] リポソーム型微小気泡(バブルリポソーム)を利用した超音波遺伝子・薬物デリバリー (2009)
  • Author(s): 鈴木亮、小田雄介、宇都口直樹、丸山一雄
  • Journal Title: マテリアルインテグレーション 22(6) Pages: 24-29
• [Journal Article] Morphological study of acoustic liposomes using transmission electron microscopy (2009)
  • Author(s): J Electron Microsc (Tokyo).
  • Journal Title: Kodama T, Tomita N, Horie S, Sax N, Iwasaki H, Suzuki R, Maruyama K, Mori S, Manabu F. Nov.11 Pages: 187-196
  • Peer Reviewed
• [Presentation] 超音波感受性リポソームを利用したがん温熱療法と免疫療法の併用によるがん治療 (2010)
  • Author(s): 鈴木亮、宇都口直樹、丸山一雄
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-09-24
• [Presentation] Cancer immunotherapy utilized ultrasound sensitive liposomes (Bubble liposomes) (2010)
  • Author(s): Ryo Suzuki
  • Organizer: PSWC2010(招待講演)
  • Place of Presentation: New Orleands, USA
• [Presentation] リポソーム技術と超音波技術を基盤とした革新的がん治療システムの構築 (2010)
  • Author(s): 鈴木亮、小田雄介、丸山一雄
  • Organizer: 化学工学会ソノプロセス分科会(招待講演)
  • Place of Presentation: 東京
• [Presentation] リポソーム製剤 (2010)
  • Author(s): 鈴木亮、丸山一雄
  • Organizer: ナノメディシン研究会(招待講演)
  • Place of Presentation: 東京
• [Presentation] Development of effective cancer therapy by the combination of ultrasound mediated mild hyperthermia and immunotherapy (2010)
  • Author(s): Ryo Suzuki, Yusuke Oda, Risa Koshima, Keiichi Hirata, Naoki Utoguchi, Kazuo Maruyama
  • Organizer: WMIC2010
  • Place of Presentation: 京都
• [Presentation] 超音波感受性リポソームを利用した新たな薬物・遺伝子送達システムの開発に関する研究 (2010)
  • Author(s): 鈴木亮
  • Organizer: 日本バイオイメージング学会(奨励賞受賞)
  • Place of Presentation: 神奈川
• [Presentation] Anti-tumor effect by the combination of therapeutic ultrasound and dendritic cell-based immunotherapy (2010)
  • Author(s): Ryo Suzuki, Naoki Utoguchi, Kazuo Maruyama
  • Organizer: 日本癌学会
  • Place of Presentation: 大阪
• [Presentation] Anti-tumor effect by the combination of liposomal bubbles and ultrasound (2010)
  • Author(s): Ryo Suzuki, Yusuke Oda, Risa Koshima, Keiichi Hirata, Naoki Utoguchi, Kazuo Maruyama
  • Organizer: Liposome Research Days 2010 (LRD2010)
  • Place of Presentation: Vancouver, Canada
• [Presentation] Development of novel antigen delivery system with liposomal bubbles (Bubble liposomes) and ultrasound in cancer immunotherapy (2010)
  • Author(s): Ryo Suzuki, Yusuke Oda, Naoki Utoguchi, Kazuo Maruyama
  • Place of Presentation: Copenhagen, Denmark
• [Presentation] Analysis of anti-tumor mechanism by the combination of therapeutic ultrasound and immunotherapy (2010)
  • Author(s): Ryo Suzuki, Yusuke Oda, Keiichi Hirata, Naoki Utoguchi, Kazuo Maruyama
  • Organizer: ISTU2010
  • Place of Presentation: 東京
• [Presentation] 超音波感受性リポソームを利用したがん温熱療法と免疫療法の併用によるがん治療 (2009)
  • Author(s): 鈴木亮、宇都口直樹、丸山一雄
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2009-10-01
• [Book] Springer Protocols, Methods in Molecular Biology (2009)
  • Author(s): Ryo Suzuki, Kazuo Maruyama
  • Total Pages: 13
  • Publisher: Effective in vitro and in vivo gene delivery by the combination of liposomal bubbles (Bubble liposomes) and ultrasound exposure
• [Remarks] ホームページ等
  • URL: http://www.pharm.teikyo-u.ac.jp/lab/s-yakuzai/index.html
• [Remarks]
  • URL: http://www.pharm.teikyo-u.ac.jp/lab/s-yakuzai/index.html
• [Remarks]
  • URL: http://www.pharm.teikyo-u.ac.jp/lab/s-yakuzai/index.html