Study on the skeletal muscle derived bioactive substances induced by muscle contraction

• Project/Area Number: 21700657
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Sports science
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: MANABE Yasuko Tokyo Metropolitan University, 人間健康科学研究科, 助教 (60467412)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 骨格筋 / 筋収縮 / ミオカイン / プロテオミクス / 分泌蛋白質

Research Abstract

In this study, a novel muscle contraction system to explore the muscle derived bioactive substances induced by muscle contraction was developed. C2C12 cells were cultured in the 10%FBS, followed by differentiation in the 2% CS differentiation medium for the 5 days. Differentiated C2C12myotubes were then contracted by a specific contraction system equipped with the carbon electrode. The medium was concentrated and candidate myokines were identified by proteomic analyses.

Research Products

• [Journal Article] 運動の糖代謝に対する急性効果の分子メカニズム (2010)
  • Author(s): 眞鍋康子, 豊田太郎, 土屋美穂, 藤井宣晴
  • Journal Title: 内分泌・糖尿病・代謝内科 31(5) Pages: 415-420
• [Journal Article] Intragastric infusion of glucose enhances the rewarding effect of sorbitol fatty acid ester ingestion as measured by conditioned place preference in mice. (2010)
  • Author(s): Matsumura S, Yoneda T, Aki S, Eguchi A, Manabe Y, Tsuzuki S, Inoue K, Fushiki T.
  • Journal Title: Physiol.Behav. 99 Pages: 509-514
  • Peer Reviewed
• [Journal Article] 運動の糖代謝に対する急性効果の分子メカニズム (2010)
  • Author(s): 眞鍋康子, 豊田太郎, 土屋美穂, 藤井宣晴
  • Journal Title: 内分泌・糖尿病・代謝内科 Volume: 31 Pages: 415-420
• [Journal Article] Intragastric infusion of glucose enhances the rewarding effect of sorbitol fatty acid ester ingestion as measured by conditioned place preference in mice (2010)
  • Author(s): Matsumura S, Yoneda T, Aki S, Eguchi A, Manabe Y, Tsuzuki S, Inoue K, Fushiki T
  • Journal Title: Physiol.Behav. Volume: 99 Pages: 509-514
  • Peer Reviewed
• [Journal Article] Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1α in skeletal muscle. (2009)
  • Author(s): Palacios M.O., Carmona J.J., Michan S., Chen Y.K., Manabe Y., Ward III L. J., Goodyear LJ., Tong Q.
  • Journal Title: AGING 1 Pages: 771-783
  • Peer Reviewed
• [Journal Article] Preference for dietary fat induced by release of beta-endorphin in rats. (2009)
  • Author(s): Mizushige T, Saitoh K, Manabe Y, Nishizuka T, Taka Y, Eguchi A, Yoneda T, Matsumura S, Tsuzuki S, Inoue K, Fushiki T.
  • Journal Title: Life Sci. 84(21-22) Pages: 760-765
  • Peer Reviewed
• [Journal Article] Assesing palatability of long-chain fatty acids from the licking behavior of BALB/c mice. (2009)
  • Author(s): Yoneda T., Saitou K., Asano H., Mizushige T., Matsumura S., Eguchi A., Manabe Y., Tsuzuki S., Inoue K., Fushiki T.
  • Journal Title: Physiol.Behav. 96 Pages: 735-741
  • Peer Reviewed
• [Journal Article] Contribution of gustation to the palatability of linoleic acid. (2009)
  • Author(s): Saitou K, Yoneda T, Mizushige T, Asano H, Okamura M, Matsumura S, Eguchi A, Manabe Y, Tsuzuki S, Inoue K, Fushiki T.
  • Journal Title: Physiol Behav. 96 Pages: 142-148
  • Peer Reviewed
• [Journal Article] Diet and exercise signals regulate SIRT3 and activate AMRK and PGC-1α in skeletal muscle. (2009)
  • Author(s): Palacios M.O., Carmona J.J., Michan S., Chen Y.K., Manabe Y., Ward III L.J., Goodyear LJ., Tong Q.
  • Journal Title: AGING 1 Pages: 771-783
  • Peer Reviewed
• [Journal Article] 筋収縮時の糖取り込みを調節する細胞内情報伝達経路. 連載;運動生理学分野の先端研究 (2009)
  • Author(s): 眞鍋康子, 豊田太郎, 藤井宣晴.
  • Journal Title: 体育の科学 59 Pages: 563-566
• [Presentation] Goodyear, Nobuharu Fujii Development of the cultured-muscle cell (C2C12) contraction system by electric stimulation (2011)
  • Author(s): Yasuko Manabe, Syouta Miyatake, Ai Okeda, Taemi Nakano, Michael M. Hirshman*, Laurie J.
  • Organizer: Inaugural International Academy of Sportology
  • Place of Presentation: 東京
  • Year and Date: 2011-03-05
• [Presentation] Development of the cultured-muscle cell(C2C12)contraction system by electric stimulation (2011)
  • Author(s): Yasuko Manabe, Syouta Miyatake, Ai Okeda, Taemi Nakano, Michael M.Hirshman, Laurie J.Goodyear, Nobuharu Fujii
  • Organizer: Inaugura Internationa-Academy of Sportology
  • Place of Presentation: 東京
  • Year and Date: 2011-03-05
• [Presentation] 骨格筋研究における新規収縮モデル系とその応用 (2010)
  • Author(s): 眞鍋康子
  • Organizer: 分子骨格筋代謝研究会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-22
• [Presentation] 骨格筋研究における新規収縮モデル系とその応用, 分子骨格筋代謝研究会, 5月22日, 2011年, 京都 (2010)
  • Author(s): 眞鍋康子
  • Organizer: 分子骨格筋代謝研究会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-22
• [Book] 改訂 基礎栄養学(摂食行動)(伏木亨、吉田宗弘編著) (2010)
  • Author(s): 眞鍋康子
  • Publisher: 光生館
• [Book] 基礎栄養学 (2010)
  • Author(s): 眞鍋康子, その他(伏木亨、吉田宗弘編著)
  • Total Pages: 13
  • Publisher: 光生館
• [Book] Taylor and Francis Group, Fat Detection(J.P.Montmayeur, J.Coutre (eds))(Fat Detection : taste, texture, and post ingestive effects) (2009)
  • Author(s): Manabe Y., Matsumura S., Fushiki T
  • Total Pages: 243
• [Book] 運動と免疫(大野秀樹、木崎節子編著)(運動と疲労・オーバートレーニングと免疫) (2009)
  • Author(s): 眞鍋康子
  • Total Pages: 247
  • Publisher: ナップ
• [Remarks] ホームページ等
  • URL: http://www.comp.tmu.ac.jp/muscle/
• [Remarks]
  • URL: http://www.comp.tmu.ac.jp/muscle/
• [Remarks]
  • URL: http://www.comp.tmu.ac.jp/muscle/index.html