Establishment of molecular mechanism of exercise motivation using a novel rat-strain featured by high levels of wheel-running activity

• Project/Area Number: 21700696
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied health science
• Research Institution: Oita University
• Principal Investigator: MORISHIMA Masaki 大分大学, 医学部, 助教 (40437934)
• Co-Investigator(Renkei-kenkyūsha): NAKAYA Yutaka 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (50136222)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: スポーツ医学 / 自発運動 / 運動意欲 / モノアミン / 心臓自律神経活動 / 心拍変動

Research Abstract

We established a novel rat strain featured by high levels of voluntary wheel-running, which is called SPORTS(Spontaneously-Running Tokushima-Shikoku) rat. The aim of this study was to investigate the effect of high-or low-intensities in voluntary exercise on sympathetic and vagal controls of heart at sedentary and during wheel-running exercise by use of SPORTS rats. At 15 weeks of age, each rat was moved to a new cage with or without an exercise wheel, and sorted into four groups ; 1) SPORTS rat with an exercise wheel(running), 2) SPORTS rat without an exercise wheel(sedentary), 3) control rat with an exercise wheel(running), and 4) control rat without an exercise wheel(sedentary). Heart rate was significantly increased in sedentary SPORTS rats compared to those in control rats. SPORTS rats show a significant reduction in resting heart rate by high-intensity running. The low frequency/high frequency(LF/HF) ratio, which reflects cardiac sympathetic nerve activity, was significantly decreased in SPORTS rats by voluntary running. However, a decrease of sympathetic nerve activity was not observed in control rats with approximately 1/10 wheel-running activity. By wheel running, the low frequency(LF) power was augmented in control rats, and was markedly depressed in SPORTS rats. High-intensity voluntary running decreases mean heart rate during resting period(sedentary) with a depression of sympathetic nerve activity. These results suggest that high-intensity voluntary exercise attenuates sympathetic nerve activity not only during exercise but also during resting period as a lasting effects.

Research Products

• [Journal Article] Nonapeptide hormones oxytocin and vasopressin distinctly regulate CaV1. 2 L-type calcium channel expression in cardiomyocytes (2010)
  • Author(s): Morishima M, Tahara S, Wang Y, Kaku T, Ono K
  • Journal Title: J Arrhythmia Volume: 26 Pages: 111-118
• [Journal Article] Nonapeptide Hormones Oxytocin and Vasopressin Distinctly Regulate Cav1.2 L-type Calcium Channel Expression in Cardiomyocytes (2010)
  • Author(s): Morishima M
  • Journal Title: Journal of Arrhythmia Volume: 26 Pages: 111-118
  • Peer Reviewed
• [Journal Article] ノナペプチドであるOxytocinとVasopressinはCav1.2-L型カルシウムイオンチャネルの発現を制御する (2010)
  • Author(s): 森島真幸、田原慎太郎、王岩、賀来俊彦、小野克重
  • Journal Title: Journal of Arrhythmia (掲載確定)(未定)
  • Peer Reviewed
• [Journal Article] υ-Adrenergic-AMPK Pathway Phosphorylates Acetyl-CoA Carboxylase in a High-epinephrine Rat Model (2009)
  • Author(s): Hattori A, Mawatari K, Tsuzuki S, Yoshioka E, Toda S, Yoshida M, Yasui S, Furukawa H, Morishima M, Ono K, Ohnishi T, Nakano M, Harada N, Takahashi A, Nakaya Y
  • Journal Title: SPORTS Obesity Volume: 18 Pages: 48-54
• [Journal Article] Telmisartan, an angiotensin II type 1 receptor antagonist, attenuates T-type Ca2+channel expression in neonatal rat cardiomyocytes (2009)
  • Author(s): Morishima M, Wang Y, Akiyoshi Y, Miyamoto S, Ono K
  • Journal Title: Eur J Pharmacol Volume: 609(1-3) Pages: 105-12
• [Journal Article] 17υ-estradiol modulates expression of low-voltage-activated CaV3. 2 T-type calcium channel via ERKs pathway in cardiomyocytes (2009)
  • Author(s): Marni F, Wang Y, Morishima M, Shimaoka T, Uchino T, Zheng M-Q, Kaku T, Ono K
  • Journal Title: Endocrinology Volume: 150(2) Pages: 879-888
• [Journal Article] Bepridil upregulates cardiac Na+channel as a long-term effect by blunting proteasome signals through inhibition of calmodulin activity (2009)
  • Author(s): Kang L, Zheng M, Morishima M, Wang Y, Kaku T, Ono K
  • Journal Title: Br J Pharmacol Volume: 157(3) Pages: 404-14
• [Journal Article] Telmisartanは心筋細胞のT型カルシウムイオンチャネルの発現を抑制する (2009)
  • Author(s): 森島真幸、王岩、秋吉裕子、宮本伸二、小野克重
  • Journal Title: European Journal of Pharmacology 609(1-3) Pages: 105-112
  • Peer Reviewed
• [Presentation] Hypoxia-inducible factor 1α (Hif-1a)による心筋T型Ca2+チャネル発現制御機構 (2011)
  • Author(s): 森島真幸、秋吉裕子、王岩、賀来俊彦
  • Organizer: 第21回日本病態生理学会
  • Place of Presentation: 日本大学医学部
  • Year and Date: 2011-08-20
• [Presentation] Effect of physical exercise on cardiac Ca^<2+> channel expression in SPORTS rat, a novel rat-strain featured by high levels of voluntary wheel-running activity (2010)
  • Author(s): Morishima M
  • Organizer: XX World Congress 2010 Kyoto ISHR
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2010-05-13
• [Presentation] Effects of physical exercise on cardiac Ca^<2+> channel expression in SPORTS rat, a novel rat-strain featured by high levels of voluntary wheel-running activity (2010)
  • Author(s): Masaki Morisima, Yan Wang, Yuko Akiyoshi, Katushige Ono
  • Organizer: 20^<th> World Congress of the International Society for Heart Research 2010 Kyoto
  • Place of Presentation: 京都市
• [Presentation] アンジオテンシン受容体を介した心筋T型カルシウムチャネルの発現抑制 (2010)
  • Author(s): 森島真幸、王岩、秋吉裕子、小野克重
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市
• [Presentation] Trancription factor Csx/Nkx2. 5 and NFAT regulate expression of Ca_V3. 2 T-type Ca^<2+> channel via protein kinase Cactions (2010)
  • Author(s): Katushige Ono, Masaki Morishima, Yan Wang
  • Organizer: 日本薬理学会年会
  • Place of Presentation: 大阪市
• [Presentation] Nonapeptide hormone oxytocin regulates Ca_V1. 2 L-type calcium channel expression in cardiomyocytes (2009)
  • Author(s): Masaki Morishima, Yan Wang, Toshihiko Kaku, Katushige Ono
  • Organizer: 第26回ISR日本部会総会
  • Place of Presentation: 札幌市
• [Presentation] Protein kinase C as a key modulator to swith isoforms from Ca_V3. 2 to Ca_V3. 1 of the T-type Ca^<2+> channel in neonatal rat cardiomyocyte (2009)
  • Author(s): Yan Wang, Masaki Morishima, Lin Kang, Toshihiko Kaku, Katushige Ono
  • Organizer: APHRS 2009(2^<nd> Asia Pacific Heart Rhytm Society Scientific Session 5^<th> Asia Pacific Atrial Fibrillation Symposium)
• [Presentation] Oxytocinは幼若ラット心筋細胞において転写因子CREBを介してL型Ca^<2+>チャネル(Ca_V1. 2)発現を抑制する (2009)
  • Author(s): 森島真幸、田原慎太郎、秋吉裕子、王岩、賀来俊彦、小野克重
  • Organizer: 第26回日本心電学会学術集会
  • Place of Presentation: 京都市
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/pathophysiology/
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/pathophysiology
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/pathophysiology
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/pathophysiology