The mechanism of exercise-induced increase in muscle insulin sensitivity
| Project/Area Number |
21700702
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied health science
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| Research Institution | Juntendo University |
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Principal Investigator |
IKEDA Shin-ichi Juntendo University, 医学(系)研究科, 研究員 (50534898)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 運動 / インスリン感受性 / マクロファージ / 骨格筋 |
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| Research Abstract |
Type 2 diabetes and obesity are characterized by insulin resistance in skeletal muscle. It has been well demonstrated that exercise increase insulin sensitivity in skeletal muscle. However, it remains still unclear how a single bout exercise enhance subsequent insulin sensitivity. Recently, it has been reported that macrophages, at least partly, regulate insulin sensitivity in several insulin target organs. We, therefore, hypothesized that macrophages are involved in the mechanisms of exercise-induced enhancement of insulin sensitivity in skeletal muscle. To test this hypothesis, we injected saline (SAL) or clodronate liposome (CL), a macrophage suppressor, to C57BL6J mice. Then, mice were subjected to a single bout of treadmill running. Twenty-four hour after exercise, we measured ex-vivo insulin-stimulated 2-deoxy glucose (DG) uptake in skeletal muscle. We observed that a single bout exercise enhanced macrophage accumulation and insulin-stimulated 2-DG uptake in plantaris muscle in SAL group. However, CL treatment completely abolished these changes. We also observed that phosphorylation state of Akt, AS160 and AMPK were not changed by CL treatment. From these results, we conclude that macrophages are involved in the mechanisms of exercise-induced enhancement of insulin sensitivity in skeletal muscle, independent of Akt and AMPK phosphorylation states.
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Report
(3 results)
Research Products
(25 results)
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[Journal Article] Determinants of intramyocellular lipid accumulation after dietary fat loading in non-obese men
Author(s)
Sakurai Y, Tamura Y, Takeno K, Kumashiro N, Sato F, Kakehi S, Ikeda S, Katamoto S, Ogura Y, Saga N, Naito H, Fujitani Y, Hirose T, Kawamori R, Watada H
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Journal Title
J Diabetes Invest In press
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[Journal Article] Determinants of intramyocellular lipid accumulation after dietary fat loading in non-obese men
Author(s)
Sakurai Y, Tamura Y, Takeno K, Kumashiro N, Sato F, Kakehi S, Ikeda S, Katamoto S, Ogura Y, Saga N, Naito H, Fujitani Y, Hirose T, Kawamori R, Watada H
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Journal Title
J Diabetes Invest
Volume: (In press)
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Peer Reviewed
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[Presentation] Susceptible Factors for Intramyocellular Lipid Accumulation by Dietary Fat Loading in Non-Obese Men.2010
Author(s)
Sakurai Y, Tamura Y, Takeno K, Kumashiro N, Sato F, Ikeda S, Kakehi S, Katamoto S, Naito H, Saga N, Ogura Y, Hirose T, Fujitani Y, Watada H
Organizer
American Diabetes Association 70^<th> Scientific sessions. Orlando
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[Presentation] 第64回日本体力医学会2009
Author(s)
池田真一、田村好史、筧佐織、綿田裕孝、河盛隆造
Organizer
廃用性筋萎縮に対するPKCδの関与の検討
Place of Presentation
ポスター
Year and Date
2009-09-20
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[Presentation] ヒトにおける高脂肪食負荷が骨格筋細胞内脂質蓄積とインスリン感受性に与える影響とその規定因子の解明
Author(s)
櫻井裕子, 田村好史, 竹野景海, 熊代尚紀, 佐藤文彦, 池田真一, 筧佐織, 形本静夫, 内藤久士, 小倉裕司, 佐賀典生, 弘世貴久, 綿田裕孝, 河盛隆造
Organizer
第53回日本糖尿病学会年次学術集会、口頭、5月
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