The Effect of Nucleoprotein diet to rheumatoid arthritis in mice

• Project/Area Number: 21700769
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Eating habits, studies on eating habits
• Research Institution: Showa University
• Principal Investigator: YOFU Sachiko 昭和大学, 医学部, 普通研究生 (00398695)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 核タンパク / 関節リウマチ / HTLV-1 Tg マウス / iNOS / 肝傷害モデルマウス / 肝障害モデルマウス / 核タンパク質 / リウマチ / 食品 / 核タンパク食 / 関節炎 / 抗酸化 / HTLV-1Tgマウス

Research Abstract

This study evaluated the effect of nucleoprotein from salmon soft roe on animal model of arthritis. Mice transgenic for human T-cell leukemia virus type I (HTLV-1 Tg) were divided into three experimental groups and supplemented on either nucleoprotein-free (nonNP), or 0.6% or 1.2% nucleoprotein mixed (NP0.6 or NP1.2) diet for 3 months. NonNP-di e t HTLV-1 Tg mice increased an arthritis symptoms and RF. The symptoms were ameliorated in NP-diet groups. Macrophages detected by F4/80 staining, and oxidative metabolites in the serum and/or joints were clearly decreased in 1.2% NP-diet HTLV-1 Tg mice. Nucleoprotein and DNA-nucleotide, but less protamine, had direct anti-oxidative potency with BAP test and/or ESR in vitro. Then to evaluate the anti-oxidative potential of NP in vivo, lipopolysaccharide (LPS)-induced liver injury model which mediating NO production was evaluated. LowNP or 1.2%NP diet was fed for two weeks before LPS (30 mg/kg) i.p. injection into the C57BL. The serum ALT, 3-NT immunoreactivity, and NO level in 1.2%NP diet group were lower than lowNP diet group.These results suggest that NP suppress a LPS-induced liver injury associated with NO.

Research Products

• [Journal Article] LPS誘導性肝傷害モデルマウスに対する核酸・核蛋白摂取の効果 (2010)
  • Author(s): 養父佐知子、小川哲郎、中町智哉、佐藤和恵、清水 藍、松永政司、塩田清二
  • Journal Title: 日本臨床生理学会誌 Volume: 40 Pages: 37-143
  • NAID: 10027752104
  • Peer Reviewed
• [Journal Article] Nucleoprotein Diet Ameliorates Arthritis Symptoms in Mice Transgenic for Human T-Cell Leukemia Virus Type I (HTLV-1) (2010)
  • Author(s): Ohtaki H, Yo f u S, Nakamachi T, Satoh K, Shimizu A, Mori H, Sato A, Iwakura Y,Matsunaga M, Shioda S.
  • Journal Title: J Clin BIochem Nutr Volume: 46 Pages: 93-104
  • Peer Reviewed
• [Journal Article] Nucleoprotein Diet Ametiorates Arthritis Symptoms in Mice Transgenic for Human T-Cell Leukemia Virus Type I (HTLV-1). (2010)
  • Author(s): Ohtaki H, Yofu S, Nakamachi T, Satoh K, Shimizu A, Mori H, Sato A, Iwakura Y, Matsunaga M, Shioda S.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition 46 Pages: 93-104
  • Peer Reviewed
• [Presentation] がん細胞に対する核タンパクの細胞増殖抑制効果 (2013)
  • Author(s): 養父 佐知子
  • Organizer: 第118回日本解剖学会総会
  • Place of Presentation: 香川
  • Year and Date: 2013-03-30
• [Presentation] 肝臓の網羅的遺伝子発現解析による核タンパク栄養の効果の検討 (2012)
  • Author(s): 養父 佐知子
  • Organizer: 第117回日本解剖学会総会
  • Place of Presentation: 山梨
  • Year and Date: 2012-03-26
• [Presentation] 肝臓の網羅的遺伝子発現解析による核タンパク栄養の効果の検討 (2012)
  • Author(s): 養父佐知子
  • Organizer: 第117回日本解剖学会総会・学術集会
  • Place of Presentation: 山梨
  • Year and Date: 2012-03-26
• [Presentation] LPS誘発肝傷害モデルマウスに対する核タンパク摂取の効果 (2010)
  • Author(s): 養父 佐知子
  • Organizer: 第115回解剖学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-03-28