Protein patterning and parallel analysis of DNA molecules by integrating nanoimprint lithography (NIL) and UV lithography (UVL)
| Project/Area Number |
21710129
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Kyoto University |
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Principal Investigator |
YOKOKAWA Ryuji Kyoto University, 大学院・工学研究科・マイクロエンジニアリング専攻, 助教 (10411216)
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| Co-Investigator(Renkei-kenkyūsha) |
笠原 賢洋 立命館大学, 生命科学部, 准教授 (70361748)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | ナノインプリント / MicroTAS / MEMS / 分子操作 / DNA |
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| Research Abstract |
We modified two ends ofλDNA by digoxigenin (DIG) and biotin for single molecule manipulation. One end labeled with DIG was immobilized on the glass surface coated by anti-DIG antibody, and the other end labeled with biotin was manipulated by gliding microtubules modified with streptavidin. After stretchingλDNA, a restriction enzyme was applied to visualize cleaving of the DNA molecule at single molecule level. To achieve highly parallel and massive DNA manipulation, we fabricated micro/nano scale perfusable multi-scale channel structures. Integration of nanoimprint lithography (NIL) and UV lithography (UVL) realized such a complex and multi-scale structures. It was evaluated by implementing a pressure-driven flow system and protein assay.
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Report
(3 results)
Research Products
(77 results)
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[Presentation] 電気浸透流を用いた異種細胞配置に関する研究2009
Author(s)
北澤裕子, 寺尾京平, 小此木孝仁, 横川隆司, 小寺秀俊
Organizer
第20回化学とマイクロナノシステム研究会(20th Cheminas)
Place of Presentation
金沢エクセルホテル東急・石川四高記念文化交流館(石川県・金沢市)
Year and Date
2009-11-08
Related Report
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