A biochemical study of cancer cell growth inhibitors from marine cyanobacteria
| Project/Area Number |
21710237
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Living organism molecular science
|
|---|
| Research Institution | University of the Ryukyus |
|---|
Principal Investigator |
TERUYA Toshiaki University of the Ryukyus, 教育学部, 准教授 (90375428)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
|---|
| Keywords | シアノバクテリア / ビセリングビアサイド / ビセブロモアミド / プロテインキナーゼ阻害 / Bisebromoamide / 細胞増殖阻害活性 / Hela S_3腫瘍細胞 / 新規鎖状ペプチド / Biselyngbyaside / 異常アミノ酸 / 鎖状ペプチド / プロテインキナーゼ阻害活性 / マクロリド配糖体 / HeLa S_3腫瘍細胞 |
|---|
| Research Abstract |
In our ongoing efforts to isolate novel marine cyanobacterial metabolites with antitumor activity, we found bisebromoamide and biselyngbyaside. Bisebromoamide exhibited potent protein kinase inhibition: the phosphorylation of ERK (extracellular signal regulated protein kinase) in NRK cells by PDGF (platelet-derived growth factor)-stimulation was selectively inhibited by treatment with 10 to 0.1μM of bisebromoamide. Bisebromoamide had no effect on the phosphorylation of AKT, PKD, PLCγ1, or S6 ribosomal protein at 10-0.1μM. Biselyngbyaside exhibited cytotoxicity against HeLa S_3 cells with an IC_<50> value of 0.1μg/mL and exhibited differential cytotoxicities : the central nervous system cancer SNB-78 (GI_<50> 0.036μM) and lung cancer NCI H522 (GI_<50> 0.067μM) were especially sensitive. Biselyngbyaside was COMPARE-negative, indicating that it likely inhibits cancer cell proliferation through a novel mechanism.
|
Report
(3 results)
Research Products
(63 results)
-
-
-
-
-
-
[Journal Article] Nobiletin improves hyperglycemia and insulin resistance in obese diabetic ob/ob mice2010
Author(s)
Lee, Y.Sil.; Cha, B.Y.; Saito, K.; Yamakawa, H.; Choi, S.S.; Yamaguchi, K.; Yonezawa, T.; Teruya, T.; Nagai, K.; Woo, J.T.
-
Journal Title
Biochemical Pharmacology 79
Pages: 1674-1683
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
