Development of cell-free synthesis and imaging of membrane proteins in giant vesicles
| Project/Area Number |
21750073
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TOYOTA Taro The University of Tokyo, 大学院・総合文化研究科, 講師 (80422377)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 生体分析 / 膜タンパク質 / ベシクル / 反射干渉顕微鏡 / 干渉顕微鏡 |
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| Research Abstract |
The aim of this study is the development of an analytical method to observe the non-equilibrium dynamics of membrane proteins incorporated into model cell membrane. Giant vesicle (GV), which is closed bilayer lipid membrane with cellular size, was used as a reactor of model cell membrane for the cell-free synthesis of a fluorescent membrane protein, eGFP-BmOR1, and the spontaneous membrane insertion of eGFP-BmOR1 synthesized inside of GV was successfully observed. Upon the construction of a reflective interference contrast microscope, the non-equilibrium dynamics of GV settling on a glass substrate was revealed in a nanometer thickness resolution. The combination of these two technologies is expected to promote further progress in the analysis of the non-equilibrium dynamics of membrane proteins and lipid membrane.
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Report
(3 results)
Research Products
(22 results)
