Discovery of novel liquid-liquid extraction system for double-stranded DNA utilizing specific interaction between ionic liquid and polyion.

• Project/Area Number: 21750075
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: Kyoto University
• Principal Investigator: NISHI Naoya Kyoto University, 大学院・工学研究科, 准教授 (10372567)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: イオン液体 / 液液抽出 / 界面イオン移動 / 促進イオン移動 / イオン対 / 液液界面 / DNA / インターカレーション

Research Abstract

We have illustrated that it is possible to create a novel liquid-liquid extraction system for double stranded DNA by applying the electrochemical concept to the ionic liquid-water two phase system and design the molecular structure of the ionic liquid-constituting cation that specifically interacts with the double stranded DNA.

Research Products

• [Journal Article] Ionic multilayers at the free surface of an ionic liquid, trioctylmethylammonium bis(nonafluorobutanesulfonyl)amide, probed by X-ray reflectivity measurements (2010)
  • Author(s): Naoya Nishi, Yukinori Yasui, Tomoya Uruga, Hajime Tanida, Tasuku Yamada, Shun-ichi Nakayama, Hideki Matsuoka, Takashi Kakiuchi
  • Journal Title: J.Chem.Phys. 132
  • NAID: 120003386584
• [Journal Article] Determination of the Activity of 1-Methyl-3-octylimidazolium Bis(trifluoromethanesulfonyl)amide in Binary Ionic Liquids from the Solubility in Water (2010)
  • Author(s): Yuko Hirohata, Naoya Nishi, Takashi Kakiuchi
  • Journal Title: J.Chem.Eng.Data 55 Pages: 1980-1985
• [Journal Article] Electrocapillarity under Ultraslow Relaxation of the Ionic Liquid Double Layer at the Interface between Trioctylmethylammonium Bis(nonafluorobutanesulfonyl)amide and Water (2010)
  • Author(s): Yukinori Yasui, Yuki Kitazumi, Naoya Nishi, Takashi Kakiuchi
  • Journal Title: J.Phys.Chem.B 114 Pages: 11141-11148
• [Journal Article] Ionic multilayers at the free surface of an ionic liquid, trioctylmethylam monium bis(nonafluorobutanesulfonyl)amide, probed by X-ray reflectivity measurements (2010)
  • Author(s): Naoya Nishi, Yukinori Yasui, Tomoya Uruga, Hajime Tanida, Tasuku Yamada, Shun-ichi Nakayama, Hideki Matsuoka, Takashi Kakiuchi
  • Journal Title: J.Chem.Phys. Volume: 132
  • NAID: 120003386584
  • Peer Reviewed
• [Journal Article] Determination of the Activity of 1-Methyl-3-octylimidazolium Bis(trifluoromethanesulfonyl)amide in Binary Ionic Liquids from the Solubility in Water (2010)
  • Author(s): Yuko Hirohata, Naoya Nishi, Takashi Kakiuchi
  • Journal Title: J.Chem.Eng.Data Volume: 55 Pages: 1980-1985
  • Peer Reviewed
• [Journal Article] Electrocapillarity under Ultraslow Relaxation of the Ionic Liquid Double Layer at the Interface between Trioctylmethylammonium Bis(nonafluorobutanesulfonyl)amide and Water (2010)
  • Author(s): Yukinori Yasui, Yuki Kitazumi, Naoya Nishi, Takashi Kakiuchi
  • Journal Title: J.Phys.Chem.B Volume: 114 Pages: 11141-11148
  • Peer Reviewed
• [Journal Article] Hydrophobic Ionic Liquids Composed of Perfluoroalkyltrifluoroborates for Ionic Liquid-Water Two-Phase Systems (2009)
  • Author(s): N.Nishi, A.Suzuki, T.Kakiuchi
  • Journal Title: Bull.Chem.Soc.Jpn. 82 Pages: 86-92
  • NAID: 10023987458
• [Journal Article] Ultraslow Response of Interfacial Tension to the Change in the Phase-Boundary Potential at the Interface between Water and a Room-Temperature Ionic Liquid, Trioctylmethylammonium bis(nonafluorobutanesulfonyl)amide (2009)
  • Author(s): Y.Yasui, Y.Kitazumi, R.Ishimatsu, N.Nishi, T.Kakiuchi
  • Journal Title: J.Phys.Chem.B 113 Pages: 3273-3276
• [Journal Article] Orientation Correlation of Sulfosuccinate-based Room Temperature Ionic Liquids Studied by Polarization Resolved Hyper Rayleigh Scattering (2009)
  • Author(s): G.Revillod, N.Nishi, T.Kakiuchi
  • Journal Title: J.Phys.Chem.B 113 Pages: 15322-15326
• [Journal Article] Ultraslow Response of Interfacial Tension to the Change in the Phase-Boundary Potential at the Interface between Water and a Room-Temperature Ionic Liquid, Trioctylmethylammonium bis(nonafluorobutanesulfonyl) amide (2009)
  • Author(s): Y.Yasui, Y.Kitazumi, R.Ishimatsu, N.Nishi, T.Kakiuchi
  • Journal Title: J.Phys.Chem.B 113 Pages: 3273-3276
  • Peer Reviewed
• [Presentation] DNAと相互作用するイソキノリニウム系カチオンを構成イオンとするイオン液体への水中DNAの電気化学的移動および抽出 (2011)
  • Author(s): 西直哉, 内田健太郎, 白波瀬文吾, 北隅優希, 垣内隆
  • Organizer: 電気化学会
  • Place of Presentation: 横浜国立大学
  • Year and Date: 2011-03-30
• [Presentation] イオン液体界面におけるイオン多層構造のX線反射率測定による検出 (2011)
  • Author(s): 西直哉
  • Organizer: 2011年春季応用物理学関係連合講演会
  • Place of Presentation: 神奈川工科大学
• [Presentation] イオン液体界面に形成されるイオン多層構造のX線反射率測定による解析:イオン種依存性 (2011)
  • Author(s): 西直哉・粕谷浩二・宇留賀朋哉・谷田肇・垣内隆
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学
• [Presentation] イオン移動ボルタンメトリーを用いて調べるイオン液体中の金属イオンとdibenzo-18-crown-6との錯生成定数の温度依存性 (2010)
  • Author(s): 松山嘉夫・西直哉・垣内隆
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学本部キャンパス
• [Presentation] Double layer structure in ionic liquids studied by electrochemistry and spectroscopy (2010)
  • Author(s): N.Nishi
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学本部キャンパス
• [Presentation] X線反射率測定による疎水性イオン液体Trioctylmethylammonium bis(nonafluorobutanesulfonyl)amide表面の層構造解析 (2010)
  • Author(s): 西直哉,安井幸則,宇留賀朋哉,谷田肇,松岡秀樹,垣内隆
  • Organizer: 電気化学会第77回大会
  • Place of Presentation: 富山大学五福キャンパス
• [Presentation] 水中DNAの疎水性イオン液体への促進イオン移動:液液界面イオン移動ボルタンメトリーによる検討 (2010)
  • Author(s): 西直哉・白波瀬文吾・北隅優希・垣内隆
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 東北大学川内北キャンパス
• [Presentation] Temperature dependence of the ionic-multilayer structure formed at the free surface of ionic liquids studied by x-ray reflectivity measurements (2010)
  • Author(s): N.Nishi, T.Uruga, H.Tanida, T.Kakiuchi
  • Organizer: NCSS2010
  • Place of Presentation: 千葉幕張メッセ
• [Presentation] イオン液体界面の特異的構造の分光学的研究 (2010)
  • Author(s): 西直哉
  • Organizer: 第56回ポーラログラフィーおよび電気分析化学討論会
  • Place of Presentation: 秋田大学
• [Presentation] Ionic multilayers at the interface of ionic liquids probed by X-ray reflectivity measurements (2010)
  • Author(s): N.Nishi, T.Kakiuchi
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA.
• [Presentation] Ultraslow relaxation of the electrical double layer at the ionic liquid| metal interface probed by surface plasmon resonance (2010)
  • Author(s): N.Nishi, T.Motokawa, Y.Kitazumi, T.Kakiuchi
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, USA.
• [Presentation] DNAと相互作用するイソキノリニウム系カチオンを構成イオンとするイオン液体への水中DNAの電気化学的移動および抽出 (2010)
  • Author(s): 西直哉,内田健太郎, 白波瀬文吾, 北隅優希, 垣内隆
  • Organizer: 電気化学会第78回大会
  • Place of Presentation: 横浜国立大学