Development of time-resolved circular dichroism measurement and its application to the detection of conformational dynamics in DNA-ligand complex

• Project/Area Number: 21750164
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Tohoku University
• Principal Investigator: ARAKI Yasuyuki 東北大学, 多元物質科学研究所, 助教 (80361179)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 生体機能関連化学 / 円二色性 / DNA / 色素

Research Abstract

We aimed to develop the time-resolved circular dichroism measurement for the purpose of the detection of the dynamic behavior of this DNA-drug interactions, focusing on an entire chirality occurring as a result of DNA-drug interaction. We have successfully developed it and detected the dynamic behavior of DNA-drug interactions caused by the laser-induced conformational change of DNA-drug complexes.

Research Products

• [Journal Article] Fullerene- and Pyromellitdiimide-Appended Tripodal Ligands Embedded in Light-Harvesting Porphyrin Macrorings (2011)
  • Author(s): Kuramochi, Y., Araki, Y, 他4名
  • Journal Title: Inorg. Chem. Volume: 50 Pages: 10249-10258
  • Peer Reviewed
• [Journal Article] Multiple photosynthetic reaction centres composed of supramolecular assemblies of zinc porphyrin dendrimers with a fullerene acceptor (2011)
  • Author(s): Fukuzumi, S., Saito, K., Ohkubo, K., Kashiwagi, Y., Araki, Y., 他6名
  • Journal Title: Chem. Commun. Volume: 47 Pages: 7980-7982
  • Peer Reviewed
• [Journal Article] Fullerene- and Pyromellitdiimide-Appended Tripodal Ligands Embedded in Light-Harvesting Porphyrin Macrorings (2011)
  • Author(s): Kuramochi, Y.; Satake, A.; Sandanayaka, A.S.D.; Araki, Y.; Ito, O.; Kobuke, Y.
  • Journal Title: Inorg.Chem. Volume: 50 Pages: 10249-10258
  • Peer Reviewed
• [Journal Article] Multiple photosynthetic reaction centres composed of supramolecular assemblies of zinc porphyrin dendrimers with a fullerene acceptor (2011)
  • Author(s): Fukuzumi, S.; Saito, K.; Ohkubo, K.; Khoury, T.; Kashiwagi, Y.; Absalom, M.A.; Gadde, S.; D'Souza, F.; Araki, Y.; Ito, O.; Crossley, M.J.
  • Journal Title: Chem.Commun. Volume: 47 Pages: 7980-7982
  • Peer Reviewed
• [Journal Article] Axle Length Effect on Photoinduced Electron Transfer in Triad Rotaxane with Porphyrin [60]Fullerene, and Triphenylamine (2010)
  • Author(s): Sandanayaka, A. S. D., Sasabe, H., Araki, Y., 他4名
  • Journal Title: Journal of Physical Chemistry A Volume: 114(16) Pages: 5242-5250
  • Peer Reviewed
• [Journal Article] The effect of atomic nitrogen on the C_<60> cage (2010)
  • Author(s): Nikawa, H., Araki, Y., 他8名
  • Journal Title: Chemical Communications (Cambridge, United Kingdom) Volume: 46(4) Pages: 631-633
  • Peer Reviewed
• [Journal Article] Role of Phe113 at the distal side of the heme domain of an oxygen-sensor(Ec DOS)in the characterization of the heme environment (2009)
  • Author(s): Ito, Shinya, Araki, Yasuyuki, Tanaka, Atsunari, Igarashi, Jotaro, Wada, Takehiko, Shimizu, Toru
  • Journal Title: Journal of Inorganic Biochemistry 103 Pages: 989-996
  • Peer Reviewed
• [Journal Article] Synthesis and Photophysical and Photovoltaic Properties of Porphyrin-Furan and-Thiophene Alternating Copolymers (2009)
  • Author(s): Umeyama, Tomokazu, Takamatsu, Takeshi, Tezuka, Noriyasu, Matano, Yoshihiro, Araki, Yasuyuki, Wada, Takehiko, Yoshikawa, Osamu, Sagawa, Takashi, Yoshikawa, Susumu, Imahori, Hiroshi
  • Journal Title: The Journal of Physical Chemistry C 113 Pages: 10798-10806
  • Peer Reviewed
• [Presentation] Ellipticity-change-detected CD apparatus toward theapplication to the DNA/RNA conformational change (2011)
  • Author(s): Yasuyuki Araki, Makoto Murakami, Yoshiki Hamada, Seiji Sakamoto, Takehiko Wada
  • Organizer: The 38th International Symposium on Nucleic Acid Chemistry (ISNAC2011)
  • Place of Presentation: Clark Memorial Student Senter, Hokkaido University, Sapporo, Japan
• [Presentation] 円二色性の高感度検出へ向けた楕円率変化検出型円二色性測定法 (2011)
  • Author(s): 荒木保幸, 村上慎, 濱田芳生, 坂本清志, 和田健彦
  • Organizer: 2011年光化学討論会
  • Place of Presentation: 宮崎市河畔コンベンションセンター
• [Presentation] 楕円率変化検出による円二色測定法のフォトンカウンティング法による高感度化 (2011)
  • Author(s): 荒木保幸, 村上慎, 坂本清志, 和田健彦
  • Organizer: 第91回日本化学会春季年会
  • Place of Presentation: 神奈川大学横浜キャンパス
• [Presentation] Ellipticity-change-detected CD apparatus toward theapplication to the DNA/RNA conformational change (2011)
  • Author(s): Yasuyuki Araki, Makoto Murakami, Yoshiki Hamada, Seiji Sakamoto, Takehiko Wada
  • Organizer: The 38^<th> International Symposium on Nucleic Acid Chemistry (ISNAC2011)
  • Place of Presentation: Clark Memorial Student Senter, Hokkaido University, Sapporo, Japan
• [Presentation] トリフェニレンをコアとする亜鉛ポルフィリン6量体の不斉軸配位子による不斉誘起とその光励起挙動 (2010)
  • Author(s): 荒木保幸, Atula Sandanayaka, Rabbani Mohamed, 羽曾部卓, 村上慎, 坂本清志, 和田健彦
  • Organizer: 第90回日本化学会春季年会
  • Place of Presentation: 関西大学(大阪府)
  • Year and Date: 2010-03-27
• [Presentation] New approach of time-resolved CD experiment for a biological application (2010)
  • Author(s): Y. Araki, M. Murakami, S. Sakamoto, T. Wada
  • Organizer: Pacifichem2010
  • Place of Presentation: Honolulu, Hawai, USA
• [Presentation] 不斉軸配位子による亜鉛ポルフィリン6量体の不斉誘起とその光励起挙動 (2010)
  • Author(s): 荒木保幸, 村上慎, 坂本清志, 和田健彦, SANDANAYAKA Atula, RABBANI Mohammad, 羽曾部卓
  • Organizer: 2010年光化学討論会
  • Place of Presentation: 千葉大学
• [Presentation] 光励起をトリガーとするDNA・カチオン性ポルフィリン錯体の動的挙動変化の時間分解CD測定法を用いた解析 (2010)
  • Author(s): 村上慎, 荒木保幸, 坂本清志, 和田健彦
  • Organizer: 2010年光化学討論会
  • Place of Presentation: 千葉大学
• [Presentation] TIME-RESOLVED CIRCULAR DICHROISM STUDY OF BINDING DYNAMICS OF H2TMPYP WITH DNA IN MICROSECOND TO MILLISECOND TIME-SCALE (2010)
  • Author(s): Yasuyuki Araki, Makoto Murakami, Seiji Sakamoto, Takehiko Wada
  • Organizer: 22nd International Symposium on Chirality
  • Place of Presentation: Sapporo, Japan
• [Presentation] 高時間分解CD測定装置を用いた二重らせんDNA-porphyrin間相互作用解析(東北大多元研) (2010)
  • Author(s): 村上慎, 荒木保幸, 坂本清志, 和田健彦
  • Organizer: 日本化学会第90春季年会(2010)
  • Place of Presentation: 近畿大学本部キャンパス
• [Presentation] トリフェニレンをコアとする亜鉛ポルフィリン6量体の不斉軸配位子による不斉誘起とその光励起挙動(東北大多元研・北陸先端大) (2010)
  • Author(s): 荒木保幸, Atula, Sandanayaka, Rabbani, Mohammad, 羽曾部卓, 村上慎, 坂本清志, 和田健彦
  • Organizer: 日本化学会第90春季年会(2010)
  • Place of Presentation: 近畿大学本部キャンパス
• [Presentation] 不斉軸配位子による亜鉛ポルフィリン6量体の不斉誘起とその光励起挙動 (2010)
  • Author(s): 荒木保幸・村上慎・坂本清志・和田健彦・SANDANAYAKA Atula・RABBANI Mohammad・羽曾部卓
  • Organizer: 2010年光化学討論会
  • Place of Presentation: 千葉大学西千葉キャンパス
• [Presentation] New approach of time-resolved CD experiment for a biological application (2010)
  • Author(s): Araki, Y.; Murakami, M.; Sakamoto, S.; Wada, T.
  • Organizer: Pacifichem 2010
  • Place of Presentation: Honolulu, Hawaii, USA
• [Presentation] DNAに結合したカチオン性ポルフィリンの時間分解円二色性測定による選択的検出 (2009)
  • Author(s): 荒木保幸, 村上慎, 坂本清志, 和田健彦
  • Organizer: 2009年光化学討論会
  • Place of Presentation: 群馬大学(群馬県)
  • Year and Date: 2009-09-18
• [Patent(Industrial Property Rights)] 円二色性測定装置 (2010)
  • Inventor(s): 荒木保幸、和田健彦、和田明生
  • Industrial Property Rights Holder: 日本分光株式会社
  • Industrial Property Number: 2010-190514
  • Filing Date: 2010-08-27
• [Patent(Industrial Property Rights)] 特許件 (2010)
  • Inventor(s): 荒木保幸、和田健彦、和田明生
  • Industrial Property Rights Holder: 日本分光株式会社
  • Industrial Property Number: 2010-190514
  • Filing Date: 2010-08-27