Molecular identification of the master regulators for nicotine biosynthesis in tobacco

• Project/Area Number: 21770046
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: SHOJI Tsubasa Nara Institute of Science and Technology, バイオサイエンス研究科, 助教 (40343272)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: アルカロイド / 転写因子 / ジャスモン酸 / タバコ / ニコチン / 制御遺伝子 / 二次代謝 / 植物

Research Abstract

Tobacco (Nicotiana tabacum) synthesizes nicotine and related pyridine alkaloids in the root, and their synthesis increases upon herbivory on the leaf via a jasmonate-mediated signaling cascade. Regulatory NIC loci that positively regulate nicotine biosynthesis have been genetically identified, and their mutant alleles have been used to breed low-nicotine tobacco varieties. Here, we studied that the NIC2 locus, comprises clustered transcription factor genes of an ethylene response factor (ERF) subfamily; in the nic2 mutant, at least seven ERF genes are deleted altogether. The NIC2-locus ERFs are close homologs of ORCA3, a jasmonate-responsive transcriptional activator of indole alkaloid biosynthesis in Catharanthus roseus, indicating that the NIC2/ORCA3 ERF subfamily was recruited independently to regulate jasmonate-inducible secondary metabolism in distinct plant lineages.

Research Products

• [Journal Article] Recruitment of a duplicated primary metabolism gene into the nicotine biosynthesis regulon in tobacco. (2011)
  • Author(s): Shoji T, Hashimoto T.
  • Journal Title: Plant J 印刷中
  • Peer Reviewed
• [Journal Article] Tobacco MYC2 regulates jasmonate-inducible nicotine biosynthesis genes directly and by way of the NIC2-locus (2011)
  • Author(s): Shoji T, Hashimoto T.
  • Journal Title: ERF genes. Plant Cell Physiol 印刷中
  • Peer Reviewed
• [Journal Article] Vacuole-localized berberine bridge enzyme-like proteins are required for a late step of nicotine biosynthesis in tobacco. (2011)
  • Author(s): Kajikawa M, Shoji T, Katoh A, Hashimoto T.
  • Journal Title: Plant Physiol 155 Pages: 2010-2022
  • Peer Reviewed
• [Journal Article] Vacuolar-localized berberine bridge enzyme-like proteins are required for a late step of nicotine biosynthesis in tobacco. (2011)
  • Author(s): Kajikawa, Masataka
  • Journal Title: Plant Physiology Volume: 155(印刷中)
  • Peer Reviewed
• [Journal Article] Clustered transcription factor genes regulate nicotine biosynthesis in tobacco. (2010)
  • Author(s): Shoji T, Kajikawa M, Hashimoto T.
  • Journal Title: Plant Cell 22 Pages: 3390-3409
  • Peer Reviewed
• [Journal Article] Clustered transcription factors regulate nicotine biosynthesis in tobacco. (2010)
  • Author(s): Shoji, Tsubasa
  • Journal Title: Plant Cell Volume: 22 Pages: 3390-3409
  • Peer Reviewed
• [Journal Article] Multidurg and toxic compound extrusion type transporters implicated in vacuolar sequestration nicotine in tobacco roots. (2009)
  • Author(s): Shoji T, Inai K, Yazaki Y, Sato Y, et. al.
  • Journal Title: Plant Physiol 149 Pages: 708-718
  • Peer Reviewed
• [Journal Article] Mulyidrug and toxic compound extrusion-type transporters implicated in vacuolar sequestration of nicotine in tobacco roots. (2009)
  • Author(s): Shoji T, Inai K, Yazaki Y, Sato Y, Takase H, Shitan N, Yazaki K, Goto Y, Toyooka K, Matsuoka K, Hashimoto T.
  • Journal Title: Plant Physiology 146 Pages: 708-718
  • Peer Reviewed
• [Presentation] 複数の転写因子が低ニコチンタバコ品種では欠失している (2011)
  • Author(s): 庄司翼、梶川昌孝、橋本隆
  • Organizer: 2010年度日本植物生理学会
  • Place of Presentation: 仙台(震災のため中止、要旨発表を持って成立)
• [Presentation] クラスター化した転写因子がタバコのニコチン生合成を制御している (2011)
  • Author(s): 庄司翼、橋本隆
  • Organizer: 2010年度日本植物生理学会
  • Place of Presentation: 仙台(震災のため中止、要旨発表を持って成立)
• [Presentation] クラスター化したERF型転写因子がタバコのニコチン生合成を制御している (2011)
  • Author(s): 庄司翼、梶川昌考、橋本隆
  • Organizer: 2010年日本農芸化学学会
  • Place of Presentation: 京都(震災のため中止、要旨発表を持って成立)
• [Presentation] Novel genes involved in nicotine biosynthesis in tobacco (2011)
  • Author(s): 加藤啓太、庄司翼、橋本隆
  • Organizer: 2010年日本農芸化学学会
  • Place of Presentation: 京都(震災のため中止、要旨発表を持って成立)
• [Presentation] ニコチン生合成に関与するタバコ新規酵素遺伝子候補の解析 (2010)
  • Author(s): 加藤啓太、庄司翼、橋本隆
  • Organizer: 2010年度日本農芸化学会関西支部大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-25
• [Presentation] Genetic analysis of alkaloid composition and translocation in wild Nicotiana species (2010)
  • Author(s): Phattharaporn P, Shoji T, Hashimoto T.
  • Organizer: 7th SOlanaceae Meeting
  • Place of Presentation: Dundee, UK
  • Year and Date: 2010-10-06
• [Presentation] Genetic analysis of alkaloid composition and translocation in wild Nicotiana species (2010)
  • Author(s): Phattharaporn, Pakdeechanuan
  • Organizer: SOL meeting
  • Place of Presentation: Dundee, UK
  • Year and Date: 2010-09-06
• [Presentation] タバコBY2培養細胞ではなぜニコチンではなくアナタビンが蓄積されるか? (2009)
  • Author(s): 庄司翼、橋本隆
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 日大(神奈川)
  • Year and Date: 2009-07-29
• [Book] Nicotine biosynthesis.(Ashihara H, Crozier AKomamine A (ed))(Plant metabolism and biotechnology) (2011)
  • Author(s): Shoji T, Hashimoto T.
  • Publisher: Wiley, New York
• [Book] Nicotine biosynthesis (Plant metabolism and biotechnology) (2011)
  • Author(s): Shoji, Tsubasa
  • Publisher: John & Sons(印刷中)
• [Book] ジャスモン酸応答:COI1-JAZ-MYC2シグナリングカスケードの分子機構植物におけるシグナル研究 (2010)
  • Author(s): 庄司翼, 橋本隆
  • Publisher: 共立出版社
• [Book] ジャスモン酸応答;COI1-JAZ-MYC2シグナリングカスケードの分子機構(植物の分子応答) (2010)
  • Author(s): 庄司翼
  • Total Pages: 5
  • Publisher: 共立出版社
• [Remarks] ホームページ等
  • URL: http://bsw3.naist.jp/hashimoto/Member/Shoji.html