Symbiosis-related genes of the nitrogen-fixing bacterium Frankia

• Project/Area Number: 21770053
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kagoshima University
• Principal Investigator: KUCHO Ken-ichi 鹿児島大学, 大学院・理工学研究科, 准教授 (70404473)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 共生 / 窒素固定 / 形質転換 / マイクロアレイ / フランキア / 部位特異的組換 / バイサルファイト / DNAメチル化 / 制限修飾系 / 次世代シーケンサー / 根粒

Research Abstract

Frankia is a nitrogen-fixing bacterium having an ability to make symbiosis with trees. Symbiosis occurs in nodules formed on roots in which Frankia fixes nitrogen and supplies the products to the host plant to promote its growth. In order to identify Frankia genes involved in the symbiosis, I attempted to establish transformation system of Frankia and globally identified Frankia genes induced in nodule.

Research Products

• [Journal Article] Characteristics of Bacteroids in Indeterminate Nodules of the Leguminous Tree Leucaena glauca (2011)
  • Author(s): Ishihara, H.
  • Journal Title: Microbes and Environments Volume: 26 Issue: 2 Pages: 156-159
  • DOI: 10.1264/jsme2.ME11104
  • NAID: 10029122935
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] Nitric oxides production induced in roots of Lotus japonicas by lipopolys accharide from Mesorhizobium loti (2011)
  • Author(s): Murakami, E.
  • Journal Title: Plant Cell Physiology Volume: 52 Issue: 4 Pages: 610-617
  • DOI: 10.1093/pcp/pcr020
  • Peer Reviewed
• [Journal Article] The Determinants of the Actinorhizal Symbiosis (2010)
  • Author(s): Kucho K, Hay A-E and Normand P.
  • Journal Title: Microbes and Environments Volume: 25 Issue: 4 Pages: 241-252
  • DOI: 10.1264/jsme2.ME10143
  • NAID: 10027419561
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] The Frankia alni symbiotic transcriptome (2010)
  • Author(s): Alloisio N, Queiroux C, Fournier P, Pujic P, Normand P, Vallenet D, Medigue C, Yamaura M, Kakoi K and Kucho K.
  • Journal Title: Mol Plant Microbe Interact Volume: 23 Issue: 5 Pages: 593-607
  • DOI: 10.1094/mpmi-23-5-0593
  • Peer Reviewed
• [Journal Article] Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions (2010)
  • Author(s): Yamaura M, Uchiumi T, Higashi S, Abe M, Kucho K.
  • Journal Title: Appl Environ Microbiol Volume: 76 Issue: 5 Pages: 1962-1964
  • DOI: 10.1128/aem.01813-09
  • Peer Reviewed
• [Journal Article] Effect of abscisic acid on symbiotic nitrogen fixation activity in the root nodules of Lotus japonicus (2010)
  • Author(s): Tominaga A, Nagata M, Futsuki K, Uchiumi T, Abe M, Kucho K, Hashiguchi M, Akashi R, Hirsch AM, Arima S, Suzuki A
  • Journal Title: Plant Signaling Behavior Volume: 5 Pages: 440-443
  • Peer Reviewed
• [Journal Article] The Frankia alni Symbiotic Transcriptome (2010)
  • Author(s): Alloisio N, Queiroux C, Fournier P, Pujie P, Normand P, Vallenet D, Medigue C, Yamaura M, Kakoi K, Kucho K
  • Journal Title: Mol Plant Microbe Interact (in press)
  • Peer Reviewed
• [Journal Article] Plant peptides govern terminal differentiation of bacteria in symbiosis (2010)
  • Author(s): Van de Velde W, Zehirov G, Szatmari A, Debreczeny M, Ishihare H, Kevei Z, Farkas H, Satiat-Jeunemaitre B, Alunni B, Bourge M, Kucho K, Abe M, Kereszt A, ,Maroti G, Uchiumi T, Kondorosi E, Mergaert P
  • Journal Title: Science 327 Pages: 1122-1126
  • Peer Reviewed
• [Journal Article] Transient transformation of Frankia by fusion marker genes in liquid culture (2009)
  • Author(s): Kucho K, Kakoi K, Yamaura M, Higashi S, Uchiumi T and Abe M.
  • Journal Title: Microbes Environ Volume: 24 Pages: 231-240
  • NAID: 10025920760
  • Peer Reviewed
• [Journal Article] Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions (2009)
  • Author(s): Yamaura M, Uchiumi T, Higashi S, Abe M, Kucho K
  • Journal Title: Appl Environ Microbiol 76 Pages: 1962-1964
  • Peer Reviewed
• [Journal Article] Enhanced nodulation and nitrogen fixation in the ABC low-sensitive mutantenf1 (enhanced nitrogen fixation 1) of Lotus japonicus (2009)
  • Author(s): Tominaga A, Nagata M, Futsuki K, Abe H, Uchiumi T, Abe M, Kucho KI, Hashiguchi M, Akashi R, Hirsch AM, Arima S, Suzuki A
  • Journal Title: Plant Physiol 151 Pages: 1965-1976
  • Peer Reviewed
• [Journal Article] Transient transformation of Frankia by fusion marker genes in liquid culture (2009)
  • Author(s): Kucho K, Kakoi K, Yamaura M, Higashi S, Uchiumi T Abe M
  • Journal Title: Microbes Environ 24 Pages: 231-240
  • NAID: 10025920760
  • Peer Reviewed
• [Presentation] 共生窒素固定放線菌フランキアのゲノム構造の多様性 (2012)
  • Author(s): 九町健一、笹川英夫、山中高史、阿部美紀子、内海俊樹
  • Organizer: 第6回日本ゲノム微生物学会年会
  • Place of Presentation: 立教大学
  • Year and Date: 2012-03-10
• [Presentation] 樹木と共生する窒素固定放線菌フランキアのゲノム解析 (2011)
  • Author(s): 九町健一
  • Organizer: 科学技術交流財団研究会「ハイスループットスクーニングと次世代DNAシーケンサーの相乗的応用」
  • Place of Presentation: 愛知
  • Year and Date: 2011-11-11
• [Presentation] 樹木と共生する窒素固定放線菌フランキアのゲノム解析 (2011)
  • Author(s): 九町健一
  • Organizer: 科学技術交流財団研究会
  • Place of Presentation: 名古屋大学(招待講演)
  • Year and Date: 2011-11-11
• [Presentation] プラスミドを用いたフランキアの形質転換 (2011)
  • Author(s): 松井勇磨, 栫健太郎, Peter Pujic, 東四郎, 阿部美紀子, Philippe Normand, 内海俊樹, 九町健一
  • Organizer: 植物微生物研究会第21回研究交流会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-21
• [Presentation] 次世代シークエンサーを用いた共生窒素固定放線菌フランキアのゲノム解析 (2011)
  • Author(s): 九町健一, 笹川英夫, 東四郎, 阿部美紀子, 内海俊樹
  • Organizer: 植物微生物研究会第21回研究交流会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-21
• [Presentation] プラスミドを用いたフランキアの形質転換 (2011)
  • Author(s): 松井勇磨, 栫健太郎, Peter Pujic, 東四郎, 阿部美紀子, Philippe Normand, 内海俊樹, 九町健一
  • Organizer: 植物微生物研究会第21回研究交流会
  • Place of Presentation: 岡山
  • Year and Date: 2011-09-20
• [Presentation] 次世代シークエンサーを用いた共生窒素固定放線菌フランキアのゲノム解析 (2011)
  • Author(s): 九町健一、笹川英夫、阿部美紀子、内海俊樹
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: (震災により要旨集発行で発表とみなされた)
• [Presentation] Isolation of uracil auxotrophic mutants in Frankia (2010)
  • Author(s): Yamaura M, Uchiumi T, Higashi S, Abe M and Kucho K.
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Miyazaki
  • Year and Date: 2010-09-20
• [Presentation] Symbiotic transcriptome of the nitrogen-fixing actinobacterium Frankia (2010)
  • Author(s): Kucho K, Alloisio N, Queiroux C, Fournier P, Pujic P, Yamaura M, Kakoi, K, Uchiumi T, Abe M and Normand P.
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Miyazaki
  • Year and Date: 2010-09-20
• [Presentation] Isolation of uracil auxotrophic mutants in Frankia (2010)
  • Author(s): Yamaura M, Uchiumi T, Higashi S, Abe M and Kucho K.
  • Organizer: 16th International Meeting on Frankia and Actinorhizal Plants
  • Place of Presentation: ポルト
  • Year and Date: 2010-09-06
• [Presentation] Use of high-activity promoters and a codon-optimized marker gene for Frankia transformation (2010)
  • Author(s): Yamaura M, Higashi S, Uchiumi T, Abe M and Kucho K.
  • Organizer: 16th International Meeting on Frankia and Actinorhizal Plants
  • Place of Presentation: ポルト
  • Year and Date: 2010-09-06
• [Presentation] Recent trials for Frankia transformation (2010)
  • Author(s): Yamaura M, Kakoi K, Iwashita M, Abe M, Uchiumi T and Kucho K.
  • Organizer: 16th International Meeting on Frankia and Actinorhizal Plants
  • Place of Presentation: ポルト
  • Year and Date: 2010-09-06
• [Presentation] Genome analysis of Frankia by next generation sequencer (2010)
  • Author(s): Kucho K, Abe M, Uchiumi T
  • Organizer: 16th International Meeting on Frankia and Actinorhizal Plants
  • Place of Presentation: ポルトガル、ポルト
  • Year and Date: 2010-09-06
• [Presentation] Recent trials for Frankia transformation (2010)
  • Author(s): Yamaura M, Kakoi K, Iwashita M, Abe M, Uchiumi T, Kucho K
  • Organizer: 16th International Meeting on Frankia and Actinorhizal Plants
  • Place of Presentation: ポルトガル、ポルト
  • Year and Date: 2010-09-06
• [Presentation] Use of high-activity promoters and a codon-optimized marker gene for Frankia transformation (2010)
  • Author(s): Kakoi K, Yamaura M, Higashi S, Uchiumi T, Abe M, Kucho K
  • Organizer: 16th International Meeting on Frankia and Actinorhizal Plants
  • Place of Presentation: ポルトガル、ポルト
  • Year and Date: 2010-09-06
• [Presentation] Isolation of uracil auxotrophic mutants in Frankia (2010)
  • Author(s): Yamaura M, Uchiumi T, Higashi S, Abe M, Kucho K
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: 宮崎県宮崎市
  • Year and Date: 2010-09-06
• [Presentation] Symbiotic transcriptome of the nitrogen fixing actinobacterium Frankia (2010)
  • Author(s): Kucho K, Alloisio N, Queiroux C, Fournier P, Pujic P, Yamaura M, Kakoi, K, Uchiumi T, Abe M, Normand P
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: 宮崎県宮崎市
  • Year and Date: 2010-09-06
• [Presentation] Genomic analysis of the actinorhizal symbiosis partners : Frankia alni and Alnus glutinosa (2010)
  • Author(s): Aloisio N, Pujic P, Fournier P, Kucho K, Hocher V, Bogusz D, Normand P
  • Organizer: XIII National Meeting of the Spanisha Society of Nitrogen Fixation and II Portuguese-Spanish Congress on Nitrogen Fixatio
  • Place of Presentation: スペイン、ザラゴザ
  • Year and Date: 2010-06-16
• [Presentation] 樹木と共生窒素固定を行う放線菌フランキアのトランスクリプトーム解析 (2010)
  • Author(s): 九町健一、Alloisio N、Queiroux C、Fournier P、Pujic P、Vallenet D、Medigue C、山浦真稔、栫健太郎、内海俊樹、阿部美紀子、Normand P
  • Organizer: 日本植物生理学会第51回大会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-20
• [Presentation] 樹木と共生窒素固定を行う放線菌フランキアのトランスクリプトーム解析 (2010)
  • Author(s): 九町健一、Alloisio N、Queiroux C、Fournier P、Pujic P、Vallenet D、Medigue C、山浦真稔、栫健太郎、内海俊樹、阿部美紀子、Normand P.
  • Organizer: 日本植物生理学会第51回大会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-19
• [Presentation] Nitrogen-fixing Symbiosis between the Actinobacterium Frankia and Trees (2010)
  • Author(s): Kucho K.
  • Organizer: 7th Okazaki Biology Conferences
  • Place of Presentation: 静岡
  • Year and Date: 2010-01-13
• [Presentation] Nitrogen-fixing Symbiosis between the Actinobacterium Frankia and Trees (2010)
  • Author(s): Kucho K
  • Organizer: 7th Okazaki Biology Conferences
  • Place of Presentation: ヤマハリゾートつま恋(静岡)
  • Year and Date: 2010-01-13
• [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用 (2009)
  • Author(s): 栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: 長野
  • Year and Date: 2009-09-20
• [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用 (2009)
  • Author(s): 栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: あがたの森文化会館(長野県)
  • Year and Date: 2009-09-09
• [Presentation] 単生で窒素固定するFrankiaで誘導される遺伝子の同定 (2009)
  • Author(s): 山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: あがたの森文化会館(長野県)
  • Year and Date: 2009-09-09
• [Presentation] Transformation of Frankia sp. strain CcI3 by fusion marker genes using liquid culture selection (2009)
  • Author(s): Kucho K, Kakoi K, Yamaura M, Higashi S, Uchiumi T, Abe M.
  • Organizer: XIV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: ケベック
  • Year and Date: 2009-07-21
• [Presentation] 共生窒素固定細菌フランキアの形質転換法の確立 (2009)
  • Author(s): 九町健一、栫健太郎、山浦真稔、内海俊樹、阿部美紀子
  • Organizer: 2009年度日本放線菌学会大会
  • Place of Presentation: 秋田
  • Year and Date: 2009-07-17
• [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用 (2009)
  • Author(s): 栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
  • Organizer: 第24回日本放線菌学会
  • Place of Presentation: 秋田
  • Year and Date: 2009-07-17
• [Presentation] Suppression subtractive hybtidization法の細菌への応用単生で窒素固定を行うFrankiaで特異的に誘導される遺伝子の探索2009年度 (2009)
  • Author(s): 山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
  • Organizer: 日本放線菌学会大会
  • Place of Presentation: 秋田
  • Year and Date: 2009-07-17
• [Presentation] 共生窒素固定細菌フランキアの形質転換法の確立 (2009)
  • Author(s): 九町健一、栫健太郎、山浦真稔、内海俊樹、阿部美紀子
  • Organizer: 2009年度 日本放線菌学会大会
  • Place of Presentation: 秋田ビューホテル(秋田県)
  • Year and Date: 2009-07-17
• [Presentation] Suppression subtractive hybtidization法の細菌への応用-単生で窒素固定を行うFrankiaで特異的に誘導される遺伝子の探索 (2009)
  • Author(s): 栫健太郎、山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
  • Organizer: 2009年度 日本放線菌学会大会
  • Place of Presentation: 秋田ビューホテル(秋田県)
  • Year and Date: 2009-07-17
• [Presentation] 共生窒素固定細菌Frankiaの形質転換法への高発現プロモーターの利用 (2009)
  • Author(s): 山浦真稔、東四郎、内海俊樹、阿部美紀子、九町健一
  • Organizer: 2009年度 日本放線菌学会大会
  • Place of Presentation: 秋田ビューホテル(秋田県)
  • Year and Date: 2009-07-17
• [Book] 土壌微生物実験法 (2012)
  • Author(s): 九町健一、笹川英夫
  • Publisher: 養賢堂(印刷中)