The significance of and the mechanism underlying the genomic multi-functionality.

• Project/Area Number: 21770068
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: Hokkaido University
• Principal Investigator: KIMURA Atsushi Hokkaido University, 大学院・理学研究院, 准教授 (90422005)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: ゲノム / クロマチン / 転写 / エピジェネティクス / 卵巣顆粒膜細胞 / noncoding RNA / 卵巣 / 精巣 / プロモーター / エンハンサー / 転写調節

Research Abstract

I investigated whether the functional regions that we identified before at three mouse gene loci have different roles in different tissues. I found that some of the regions showed different activities to regulate gene transcription in different tissues. This difference is probably attributed to the epigenetic status and the transcription of the regulatory sequence itself.

Research Products

• [Journal Article] Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes : Possible involvement in ovulation. (2011)
  • Author(s): Fujimori C., Ogiwara K., Hagiwara A., Rajapakse S., Kimura A., Takahashi T.
  • Journal Title: Mol.Cell.Endocrinol. 332 Pages: 67-77
  • NAID: 120002834743
  • Peer Reviewed
• [Journal Article] Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes : Possible involvement in ovulation. (2011)
  • Author(s): Fujimori C., Ogiwara K., Hagiwara A., Rajapakse S., Kimura A., Takahashi T.
  • Journal Title: Molecular and Cellular Endocrinology Volume: 332 Pages: 67-77
  • NAID: 120002834743
  • Peer Reviewed
• [Journal Article] Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression. (2010)
  • Author(s): Matsubara S., Takahashi T., Kimura A.P.
  • Journal Title: Gene 465 Pages: 17-29
  • Peer Reviewed
• [Journal Article] Expression and localization of collagen type IV αI chain in the medaka ovary. (2010)
  • Author(s): Kato Y., Ogiwara K., Fujimori C., Kimura A., Takahashi T.
  • Journal Title: Cell Tissue Res. 340 Pages: 595-605
  • Peer Reviewed
• [Journal Article] Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression. (2010)
  • Author(s): Matsubara S., Takahashi T., Kimura A.P.
  • Journal Title: Gene Volume: 465 Pages: 17-29
  • Peer Reviewed
• [Journal Article] Expression and localization of collagen type IV αI chain in the medaka ovary (2010)
  • Author(s): Kato Y., Ogiwara K., Fujimori C., Kimura A., Takahashi T.
  • Journal Title: Cell and Tissue Research (未定, 印刷中)
  • Peer Reviewed
• [Journal Article] Two distinct localization patterns of testis-specific serine protease 1(TESSP1)in the seminiferous tubules of the mouse testis (2009)
  • Author(s): Takano N., Kimura A., Takahashi T.
  • Journal Title: Zoological Sciences 26 Pages: 294-300
  • NAID: 40016615722
  • Peer Reviewed
• [Journal Article] Localization and subcellular distribution of prolyl oligopeptidase in the mouse placenta.
  • Author(s): Matsubara S., Takahashi T., Kimura A.P.
  • Journal Title: J.Mol.Histol. (印刷中)
  • Peer Reviewed
• [Presentation] マウス精巣特異的TESSPクラスターの発現調節機構 (2010)
  • Author(s): 米田竜馬、木村敦
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-09-23
• [Presentation] マウスの卵巣におけるプロリルオリゴペプチダーゼのエピジェネティックな発現調節 (2010)
  • Author(s): 松原伸、木村敦
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-09-23
• [Presentation] ミュラー管抑制因子受容体遺伝子の転写調節メカニズム (2010)
  • Author(s): 木村敦
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-09-23
• [Presentation] 哺乳類におけるTCAM 遺伝子の発現パターンと調節機構 (2010)
  • Author(s): 栗原美寿々、木村敦
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-09-23
• [Presentation] 哺乳類におけるTCAM遺伝子の発現パターンと調節機構 (2010)
  • Author(s): 栗原美寿々、木村敦
  • Organizer: 第81回日本動物学会大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-09-23
• [Presentation] Epigenetic regulation and hormonal control of the mouse prolyl oligopeptidase gene in the ovary. (2010)
  • Author(s): Matusbara S., Kimura A.P.
  • Organizer: The Endocrine Society, 92nd Annual Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-06-21
• [Presentation] Characterization of two DNase I hypersensitive sites at the mouse Amhr2/MISIIR gene locus. (2010)
  • Author(s): Kimura A.P.
  • Organizer: The Endocrine Society, 92nd Annual Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-06-21
• [Presentation] The expression pattern and regulation of testis specific serine protease (TESSP) genes during spermatogenesis. (2010)
  • Author(s): Yoneda R., Kurihara M., Takahashi T., Kimura A.P.
  • Organizer: The Endocrine Society, 92nd Annual Meeting
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2010-06-20
• [Presentation] DNAメチル化によるハウスキーピング遺伝子の発現量の調節 (2009)
  • Author(s): 松原伸、木村敦
  • Organizer: 第34回日本比較内分泌学会年会
  • Place of Presentation: 大阪千里ライフサイエンスセンター
  • Year and Date: 2009-10-23
• [Presentation] マウス精巣特異的TESSPクラスターの発現解析 (2009)
  • Author(s): 米田竜馬、高橋孝行、木村敦
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-17
• [Presentation] マウスの初期発生におけるプロリルオリゴペプチダーゼのエピジェネティックな発現調節 (2009)
  • Author(s): 松原伸、木村敦
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 静岡
  • Year and Date: 2009-09-17
• [Presentation] マウス精巣特異的TESSPクラスターの発現解析 (2009)
  • Author(s): 米田竜馬、高橋孝行、木村敦
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 東静岡市
  • Year and Date: 2009-09-17
• [Presentation] マウスの初期発生におけるプロリルオリゴペプチダーゼのエピジェネティックな発現調節 (2009)
  • Author(s): 松原伸、木村敦
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 東静岡市
  • Year and Date: 2009-09-17