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Gemone instability induced by non-coding RNA expression

Research Project

Project/Area Number 21770193
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionNational Institute of Genetics

Principal Investigator

IIDA Tetsushi  National Institute of Genetics, 細胞遺伝研究系, 助教 (60391851)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
KeywordsノンコーディングRNA / サイレンシング / RNA干渉 / 分裂酵母
Research Abstract

Cryptic transcripts transcribed from everywhere in eukaryotic chromosomes are rapidly degraded by RNA surveillance mechanisms before translation. Defects in RNA surveillance mechanisms result in aberrant accumulation of noncoding RNAs (ncRNAs) and genome instability. Conversely, how excessively transcribed ncRNAs affect genome stability and gene expression is not known. In this study, we developed an artificial ncRNA-expression system of the ura4^+ reporter gene in fission yeast. One of the strongest promoters, nmt1p, induced antisense RNA of the ura4^+ gene and ura4^+ gene silencing. The ncRNA-induced silencing did not associate with hinstone-H3K9 methylation or accumulation of heterochromatin protein, Swi6, at silenced locus. These results suggest that the ncRNA-induced silencing is regulated by different mechanisms from the heterochromatic silencing. However, interestingly, the RNAi machineries involved in heterochromatic silencing were required for not ncRNA-induced silencing but normal growth of cells excessively expressing ncRNAs. This study proposed that the RNAi mechanism contribute to the RNA surveillance and suppression of genome instability.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report

Research Products

(3 results)

All 2010 Other

All Presentation Remarks

  • [Presentation] RNAi mediated cell cycle regulation in fission yeast2010

    • Author(s)
      飯田哲史、筒井康博
    • Organizer
      第32回日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] RNAi mediated cell cycle regulation in fission yeast2010

    • Author(s)
      飯田哲史
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.nig.ac.jp/labs/CytoGen/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-03-31   Modified: 2016-04-21  

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