Functional analysis of Rab33B in autophagy.

• Project/Area Number: 21770198
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Tohoku University
• Principal Investigator: ITOH Takashi Tohoku University, 大学院・生命科学研究科, 助教 (50373270)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: タンパク質分解 / 膜輸送 / オートファジー / Rab33 / ゴルジ体 / Atg16L

Research Abstract

The direct interaction of Rab33B, a regulator of membrane trafficking, with Atg16L1, an essential factor for autophagy, suggests involvement of Rab33B in the regulation of autophagy. Here, I identified OATL1, an uncharacterized TBC protein, as an inactivation factor of Rab33B and addressed the function of Rab33B in autophagy to inactivate Rab33B by OATL1. Overexpression of OATL1, which would inactivate endogenous Rab33B, delayed autophagosomal maturation in MEF cells. On the other hand, overexpression of active-form mutant of Rab33B also delayed autophagosomal maturation. These results indicate that Rab33B plays a role in the fusion between autophagosomes and lysosomes, a later process of autophagy.

Research Products

• [Journal Article] OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation. (2011)
  • Author(s): Itoh, T., Kanno, E., Uemura, T., Waguri, S., Fukuda, M.
  • Journal Title: J.Cell Biol. 192 Pages: 839-853
  • Peer Reviewed
• [Journal Article] OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation. (2011)
  • Author(s): 伊藤敬, 他
  • Journal Title: The Journal of Cell Biology Volume: 192 Pages: 839-853
  • Peer Reviewed
• [Journal Article] Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. (2009)
  • Author(s): Tamura, K., Ohbayashi, N., Maruta, Y., Kanno, E., Itoh, T., Fukuda, M.
  • Journal Title: Mol.Biol.Cell 20 Pages: 2900-2908
  • Peer Reviewed
• [Presentation] Search for Rab-GAP involved in autophagy. (2011)
  • Author(s): 伊藤敬
  • Organizer: 6th Autophagy Conference
  • Place of Presentation: 掛川、静岡、日本
  • Year and Date: 2011-01-13
• [Presentation] Identification of Rab-GAP involved in autophagy. (2010)
  • Author(s): Itoh, T., Kanno, E., Uemura, T., Waguri, S., Fukuda, M.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Search for Rab-GAP involved in autophagy. (2010)
  • Author(s): Itoh, T.
  • Organizer: 6th Autophagy Conference
  • Place of Presentation: Kakegawa, Japan
  • Year and Date: 2010-01-13
• [Presentation] Identification of Rab-GAP involved in autophagy. (2009)
  • Author(s): Itoh, T., Kanno, E., Fukuda, M.
  • Organizer: 新学術領域研究「細胞内ロジスティクス」班会議
  • Place of Presentation: 沖縄
  • Year and Date: 2009-11-10
• [Presentation] Identification of a novel Rab-GAP involved in autophagy (2009)
  • Author(s): 伊藤敬
  • Organizer: 細胞内ロジスティクス班会議
  • Place of Presentation: 沖縄県国頭郡恩納村