Functional analysis of actin-binding protein Tropomyosin4 in the spindle formation and orientation

• Project/Area Number: 21770200
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: NISHIZUMI Noriko 東京大学, 医科学研究所, 技術専門職員 (30396882)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 減数分裂 / 紡錘体 / リン酸化 / アクチン / マウス卵 / アクチンフィラメント / トロポミオシン

Research Abstract

The mammalian oocyte is highly polarized because asymmetrical spindle migration to the oocyte cortex ensures extrusion of small polar bodies in the two meiotic divisions. To elucidate the mechanism of maintain this polarity, I analyzed the function of an actin-filament associated protein tropomyosin 4(Tm4). Tm4-depletion by siRNA causes abnormal spindles with diffused poles and polarity of spindles in oocyte. These data indicate that Tm4 plays important roles in spindle orientation and chromosome migration during meiosis.

Research Products

• [Presentation] トロポミオシン4による紡錘体と分裂期細胞表層アクチン構造の制御 (2012)
  • Author(s): 渡海-西住紀子
  • Organizer: 第29回染色体ワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 2012-01-25
• [Presentation] Tropomyosin 4, actin binding protein, contributes to the proper spindle orientation during mitosis and meiosis (2011)
  • Author(s): Noriko Tokai-Nishizumi
  • Organizer: 2011 ASCB Annual Meeting (The American Society for Cell Biology)
  • Place of Presentation: デンバーコンベンションセンターUSA
  • Year and Date: 2011-12-05
• [Presentation] Tropomyosin 4, Actin binding protein, contributes to the proper spindle orientation during mitosis and meiosis (2011)
  • Author(s): Noriko Tokai-Nishizumi
  • Organizer: 2011 Annual Meeting The American Society for Cell Biology
  • Place of Presentation: Colorado Convention Center
  • Year and Date: 2011-12-03
• [Presentation] トロポミオシン4は細胞分裂期表層アクチン構造の安定性と紡錘体極性に寄与する/toropomyosin 4 contributes to the proper spindle orientation and cortical actin organization during mitosis (2011)
  • Author(s): 渡海紀子
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-29
• [Presentation] トロポミオシン4は細胞分裂期表層アクチン構造の安定性と紡錘体極性に寄与する/Tropomyosin 4 contributes to the proper spindle orientation and cortical actin organization during mitosis (2011)
  • Author(s): 渡海(西住)紀子
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-06-29
• [Presentation] アクチン結合蛋白質トロポミオシン4の紡錘体形成過程における機能 (2011)
  • Author(s): 渡海紀子
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 石川
  • Year and Date: 2011-01-11
• [Presentation] アクチン結合蛋白質トロポミオシン4の紡錘体形成過程における機能 (2011)
  • Author(s): 渡海(西住)紀子
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 加賀山代温泉 瑠璃光
  • Year and Date: 2011-01-11
• [Presentation] Tropomyosin 4 contributes to the proper spindle orientation and cortical actomyosin organization during mitosis (2011)
  • Author(s): 渡海紀子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Tropomyosin 4 contributes to the proper spindle orientation and cortical actomyosin organization during mitosis (2011)
  • Author(s): 渡海(西住)紀子
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Toropomyosin 4, a possible substrate of polo-like kinase 1, contributes to the proper spindle orientation and cortical actin organization during mitosis (2010)
  • Author(s): Noriko Tokai-Nishizumi
  • Organizer: 第16回武田科学振興財団生命科学シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-12-01
• [Presentation] (2010)
  • Author(s): 渡海-西住紀子
  • Organizer: Global COE International symposium 2010
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-05
• [Presentation] アクチン結合蛋白質トロポミオシン4の紡錘体形成過程における機能 (2010)
  • Author(s): 渡海(西住)紀子
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 御殿場 時之栖
  • Year and Date: 2010-01-21
• [Presentation] アクチン結合蛋白質トロポミオシン4の紡錘体形成過程における機能 (2010)
  • Author(s): 渡海-西住紀子
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 御殿場
• [Presentation] Toropomyosin 4, a possible substrate of polo-like kinase 1, contributes to the proper spindle orientation during mitosis (2009)
  • Author(s): Noriko Tokai-Nishizumi
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Toropomyosin 4, a possible substrate of polo-like kinase 1, contributes to the proper spindle orientation during mitosis (2009)
  • Author(s): 渡海(西住)紀子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] polo-like kinase 1の基質候補トロポミオシン4は細胞分裂過程の紡錘体の位置に寄与している (2009)
  • Author(s): Noriko Tokai-Nishizumi
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Presentation] polo-like kinase 1の基質候補トロポミオシン4は細胞分裂過程の紡錘体の位置に寄与している (2009)
  • Author(s): 渡海(西住)紀子
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-04