Development of novel efficient techniques for genetic manipulation and their application to explore the mechanisms of body axis determination in the cricket Gryllus bimaculatus
| Project/Area Number |
21770237
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MITO Taro The University of Tokushima, 大学院・ソシオテクノサイエンス研究部, 助教 (80322254)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 遺伝子導入 / 昆虫 / 体軸決定 |
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| Research Abstract |
We established techniques of genetic modification (transgenesis and targeted mutagenesis) in the cricket Gryllus bimaculatus, of which the modes of oogenesis and embryogenesis differ from those of Drosophila. Using the transgenic cricket system, we investigated cell behaviors during early embryogenesis via live imaging of fluorescently labeled embryonic cells/nuclei and revealed very dynamic features of blastoderm cells. Furthermore, results from our live imaging analysis of RNAi-treated embryos suggested that the presumptive embryonic cells are regionally specified by genes such as orthodenticle before the dynamic cell movement in late blastoderm stages.
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Report
(3 results)
Research Products
(26 results)
