Functional analysis of nucleostemin in pluripotent stem and tissue stem cells
Project/Area Number |
21770242
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Single-year Grants |
Research Field |
Developmental biology
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Research Institution | Keio University |
Principal Investigator |
NAGAMATSU Go Keio University, 医学部, 助教 (70453545)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 幹細胞 / 自己複製 |
Research Abstract |
We succeeded in making the conditional Nucleostemin deficient embryonic stem cells by a consecutive targeting of two times. Upon deletion of Nucleostemin allele, stop of proliferation and cell death had found. Moreover, it was clarified that the genes that did an important working to the maintenance of the undifferentiation of the embryonic stem cell, such as Oct3/4, Nanog, and Sox2 decreased along with the Nucleostemin loss. The possibility of the inducement or maintaining of the factors that Nucleostemin is related to the above-mentioned undifferentiated maintenance is suggested from this. On the other hand when Nucleostemin was over-expressed, the up-regulation of pluripotency associated genes was observed.
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Report
(3 results)
Research Products
(6 results)
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[Journal Article] A germ cell specific gene, Prmt5 works as somatic cell reprogramming2011
Author(s)
Nagamatsu G, Kosaka T, Kawasumi M, Kinoshita T, Takubo K, Akiyama H, Sudo T, Kobayashi T, Oya M, Suda T.
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Journal Title
J Biol Chem. 286(12)
Pages: 10641-10648
Related Report
Peer Reviewed
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[Journal Article] A germ cell specific gene, Prmt5 works as somatic cell reprogramming2011
Author(s)
Nagamatsu G, Kosaka T, Kawasumi M, Kinoshita T, Takubo K, Akiyama H, Sudo T, Kobayashi T, Oya M, Suda T.
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Journal Title
J Biol Chem.
Volume: 286(12)
Pages: 10641-8
Related Report
Peer Reviewed
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