Functional analysis of nucleostemin in pluripotent stem and tissue stem cells

• Project/Area Number: 21770242
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Keio University
• Principal Investigator: NAGAMATSU Go Keio University, 医学部, 助教 (70453545)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 幹細胞 / 自己複製

Research Abstract

We succeeded in making the conditional Nucleostemin deficient embryonic stem cells by a consecutive targeting of two times. Upon deletion of Nucleostemin allele, stop of proliferation and cell death had found. Moreover, it was clarified that the genes that did an important working to the maintenance of the undifferentiation of the embryonic stem cell, such as Oct3/4, Nanog, and Sox2 decreased along with the Nucleostemin loss. The possibility of the inducement or maintaining of the factors that Nucleostemin is related to the above-mentioned undifferentiated maintenance is suggested from this. On the other hand when Nucleostemin was over-expressed, the up-regulation of pluripotency associated genes was observed.

Research Products

• [Journal Article] A germ cell specific gene, Prmt5 works as somatic cell reprogramming (2011)
  • Author(s): Nagamatsu G, Kosaka T, Kawasumi M, Kinoshita T, Takubo K, Akiyama H, Sudo T, Kobayashi T, Oya M, Suda T.
  • Journal Title: J Biol Chem. 286(12) Pages: 10641-10648
  • Peer Reviewed
• [Journal Article] Ataxia-telangiectasia mutated (ATM) deficiency decreases reprogramming efficiency and leads to genomic instabilityin iPS cells. (2011)
  • Author(s): Kinoshita T, Nagamatsu G, Kosaka T, Takubo K, Hotta A, Ellis J, Suda T.
  • Journal Title: Biochem Biophys Res Commun. 407(2) Pages: 321-326
  • Peer Reviewed
• [Journal Article] A germ cell specific gene, Prmt5 works as somatic cell reprogramming (2011)
  • Author(s): Nagamatsu G, Kosaka T, Kawasumi M, Kinoshita T, Takubo K, Akiyama H, Sudo T, Kobayashi T, Oya M, Suda T.
  • Journal Title: J Biol Chem. Volume: 286(12) Pages: 10641-8
  • Peer Reviewed
• [Journal Article] Ataxia-telangiectasia mutated (ATM) deficiency decreases reprogramming efficiency and leads to genomic instability in iPS cells. (2011)
  • Author(s): Kinoshita T, Nagamatsu G, Kosaka T, Takubo K, Hotta A, Ellis J, Suda T.
  • Journal Title: Biochem Biophys Res Commun. Volume: 407(2) Pages: 321-6
  • Peer Reviewed
• [Presentation] 始原生殖細胞からの多能性幹細胞分化 (2011)
  • Author(s): 永松剛・小坂威雄・田久保圭誉・大家基嗣・須田年生
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 京王プラザホテル(新宿)
  • Year and Date: 2011-03-02
• [Presentation] 始原生殖細胞からの多能性幹細胞分化 (2011)
  • Author(s): 永松剛・小坂威雄・田久保圭誉・大家基嗣・須田年生
  • Organizer: 第10回 日本再生医療学会総会
  • Place of Presentation: 京王プラザホテル(新宿)
  • Year and Date: 2011-03-02