Characterization of the skin fibroblast, and induction of the artificial wound epidermis aiming for limb regeneration in mice

• Project/Area Number: 21770244
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Aichi Gakuin University
• Principal Investigator: ENDO Tetsuya Aichi Gakuin University, 教養部, 講師 (90399816)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: 四肢再生 / 器官再生 / 再生生物学 / 哺乳類 / 器官培養 / 創傷治癒 / 繊維芽細胞 / 傷表皮 / 器宮培養

Research Abstract

To clarify if mammalians can be induced to regenerate their limbs like amphibians do, two things were examined in neonatal mice as below. 1) Do skin fibroblasts maintain pattern formation potency? 2) Can the amphibian-type wound epidermis be formed on the amputation stump? In the organ culture system, even in mice, the amputation surface was covered by thin epidermal sheet within a few days, and the morphology of the sheet was similar to the amphibian-type wound epidermis. Those results suggest that the early events of amphibian limb regeneration might be inducible in neonatal mice

Research Products

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