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Experimental verification of the requirement of micro-environment for continuous self-replication system.

Research Project

Project/Area Number 21770256
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Evolutionary biology
Research InstitutionOsaka University

Principal Investigator

ICHIHASHI Norikazu  Osaka University, 大学院・情報科学研究科, 招聘教員 (20448096)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords細胞サイズ / エマルション / 自己複製 / RNA複製 / 寄生体 / 無細胞翻訳系 / RNA / マイクロ流路 / 複製酵素
Research Abstract

Most of the self-sustained cell are micro-meter size ; there are no larger cells or smaller cells. In order to investigate the importance of micro-size of cells, I constructed an artificial self-replication system of genetic information, and examine the size-dependency of it. I found that the replication proceeded continuously only in micro-size reaction volume because of the repression of the propagation of parasitic replicator. This result suggested that the cell size is strongly connected with defense mechanism against parasite.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (10 results)

All 2010 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Book (2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Identification of two forms of Qβ replicase with different thermal stabilities but identical RNA replication activity2010

    • Author(s)
      Norikazu Ichihashi, Tomoaki Matsuura, Kazufumi Hosoda, Tetsuya Yomo
    • Journal Title

      The Journal of Biological Chemistry 285

      Pages: 37210-37217

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Compartmentalization in a water-in-oil emulsion repressed the spontaneous amplification of RNA by Qβ replicase2010

    • Author(s)
      Hiroya Urabe, Norikazu Ichihashi, Tomoaki Matsuura, Kazufumi Hosoda, Yasuaki. Kazuta, Hiroshi Kita, Tetsuya Yomo
    • Journal Title

      Biochemistry 49

      Pages: 1809-1813

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of two forms of Qβrephcase with different thermal stabilities but identical RNA replication activity2010

    • Author(s)
      市橋伯一
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 285 Pages: 37210-37217

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Constructing an artificial self-replication system of genetic information from RNA and proteins2010

    • Author(s)
      市橋伯一
    • Organizer
      12th International Conference on the Synthesis and Simulation of Living Systems (Alife XII)
    • Place of Presentation
      Odense, Denmark
    • Year and Date
      2010-08-22
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Constructing self-replication system in liposome.2009

    • Author(s)
      市橋伯一
    • Organizer
      International Symposium on Complex System Biology
    • Place of Presentation
      東京
    • Year and Date
      2009-09-29
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Book] The Origins of Life2010

    • Author(s)
      Norikazu Ichihashi, Tomoaki Matsuura, Hiroshi Kita, Takeshi Sunami, Hiroaki Suzuki, Tetsuya Yomo
    • Publisher
      COLD SPRING HARBOR LABORATORY PRESS
    • Related Report
      2010 Final Research Report
  • [Book] The Origins of Life2010

    • Author(s)
      市橋伯一
    • Publisher
      COLD SPRING HARBOR LABORATORY PRESS
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www-symbio.ist.osaka-u.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www-symbio.ist.oska-u.ac.jp/

    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] エマルジョンを用いたRNAの選択的増幅法2009

    • Inventor(s)
      四方哲也、松浦友亮、市橋伯一、占部博也
    • Industrial Property Rights Holder
      科学技術振興機構
    • Industrial Property Number
      2009-168285
    • Filing Date
      2009-07-16
    • Related Report
      2010 Final Research Report 2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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