Identification of genes relating to late megagametogenesis in rice

• Project/Area Number: 21780008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: National Institute of Genetics
• Principal Investigator: KUBO Takahiko 国立遺伝学研究所, 系統生物研究センター, 助教 (00370148)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 胚のう / イネ / 生殖的隔離

Research Abstract

Little is known about molecules, and the molecular networks involved in megagametogenesis of higher plants. In order to identify genes involved in rice megagametogenesis, transcriptome and proteome analyses of female sterile lines were carried out in this study. Comparison analyses of sterile and normal ovules revealed 214 genes that should be involved in rice megagametogenesis.

Research Products

• [Journal Article] Hybrid male sterility in rice is due to epistatic interactions with a pollen killer locus (2011)
  • Author(s): Kubo T, Yoshimura A, Kurata N.
  • Journal Title: Genetics Volume: 189 Pages: 1083-1092
  • Peer Reviewed
• [Journal Article] Rice expression atlas in reproductive development (2010)
  • Author(s): Fujita, M., Horiuchi, Y., Ueda, Y., Mizuta, Y., Kubo, T., et al.
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 2060-2081
  • NAID: 10028056583
  • Peer Reviewed
• [Journal Article] Rice expression atlas in reproductive development. (2010)
  • Author(s): Fujita M
  • Journal Title: Plant Cell Physiol. Volume: Vol.51 Pages: 2060-2081
  • Peer Reviewed
• [Journal Article] Identification of two loci causing F1 pollen sterility in inter and intraspecific crosses of rice (2009)
  • Author(s): Win, K. T., Kubo, T., Miyazaki, Y., Doi, K., Yamagata, Y. and Yoshimura, A.
  • Journal Title: Breeding Science Volume: 59 Pages: 411-418
  • NAID: 130004146204
  • Peer Reviewed
• [Presentation] イネ雌性不稔に関わる遺伝子発現プロファイリング (2012)
  • Author(s): 久保貴彦、藤田雅丈、倉田のり
  • Organizer: 第121回日本育種学会講演会
  • Place of Presentation: 宇都宮
• [Presentation] イネPollen killerを制御するエピスタシスの解析 (2011)
  • Author(s): 久保貴彦
  • Organizer: 日本育種学会第119回講演会
  • Place of Presentation: 横浜市(神奈川県)
  • Year and Date: 2011-03-30
• [Presentation] イネ雌性配偶子形成過程のトランスクリプトーム解析 (2011)
  • Author(s): 久保貴彦、藤田雅丈、高橋宏和、中園幹生、堤伸浩、倉田のり
  • Organizer: 第120回日本育種学会講演会
  • Place of Presentation: 福井
• [Presentation] イネPollen killerを制御するエピスタシスの解析 (2011)
  • Author(s): 久保貴彦、倉田のり
  • Organizer: 第119回日本育種学会講演会
  • Place of Presentation: 横浜
• [Presentation] Genetic and genomic analysis of factors consisting of reproductive barriers in rice (2009)
  • Author(s): 倉田のり
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-11
• [Presentation] Genome evolution and reproductive barriers in rice (2009)
  • Author(s): Nori Kurata, Yoko Mizuta, Takahiko Kubo, Yoshiaki Harushima
  • Organizer: 6th International Rice Genetics Symposium
  • Place of Presentation: マニラ、フィリピン
• [Presentation] イネ生殖細胞の発生に関わる遺伝子群の解析 (2009)
  • Author(s): 久保貴彦
  • Organizer: 国立遺伝学研究所研究集会
  • Place of Presentation: 三島
• [Presentation] Genetic and genomic analysis of factors consisting of reproductive barriers in rice (2009)
  • Author(s): 倉田のり、久保貴彦、水多陽子、新濱充、藤田雅丈、大柳一、望月孝子、春島嘉章
  • Organizer: 第32回年会日本分子生物学会年会ワークショップ
  • Place of Presentation: 横浜
• [Presentation] イネ全生殖過程における遺伝子発現解析を用いた多様な因子の捕捉 (2009)
  • Author(s): 藤田雅丈、堀内陽子、上田弥生、水多陽子、久保貴彦、矢野健太郎、山木辰一郎、津田勝利、新濱充、加藤大貴、菊地俊介、濱田和輝、望月孝子、堤伸浩、倉田のり
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜