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Suppression of floral development in high temperature condition

Research Project

Project/Area Number 21780028
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Horticulture/Landscape architecture
Research InstitutionIwate Biotechnology Research Center

Principal Investigator

NAKATSUKA Takashi  Iwate Biotechnology Research Center, 細胞工学研究部, 主任研究員 (60435576)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords花器官形成 / 高温障害 / MADS-box / リンドウ / MADS box / 遺伝子組換え / 生理障害
Research Abstract

Six B-class MADS-box genes (GsDEF1-3, GsGLO1-3) were isolated from the petals of Japanese gentian. Yeast two-hybrid analysis showed that GsGLO2 interacted with both GsDEF1 and GsDEF2. In low-temperature condition, the interaction intensities between GsGLO2 and GsDEF1/2 were increased. The transgenic Arabidopsis and tobacco plants co-overexpressing GsGLO2 and GsDEF1/2 induced the conversion of sepal into petal-like organs, but the relationships between in vivo protein-to-protein interaction and temperature could not be elucidated. Now, I am producing transgenic gentian plants, and further studies will make clear these problems.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report

Research Products

(9 results)

All 2010 Other

All Presentation (6 results) Remarks (3 results)

  • [Presentation] 遺伝子組換えによるリンドウの多様化の可能性-花色、形態から開花期まで-2010

    • Author(s)
      中塚貴司・齋藤美沙・山田恵理・木村惣一・西原昌宏
    • Organizer
      植物細胞分子生物学会第28回大会・シンポジウム
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-03
    • Related Report
      2010 Final Research Report
  • [Presentation] リンドウから単離したMADS box 遺伝子の機能解析2010

    • Author(s)
      齋藤美沙・中塚貴司・西原昌宏
    • Organizer
      植物細胞分子生物学会第28回大会・シンポジウム
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-03
    • Related Report
      2010 Final Research Report
  • [Presentation] 遺伝子組換えによるリンドウの多様化の可能性 -花色、形態から開花期まで-2010

    • Author(s)
      中塚貴司・齋藤美沙・山田恵理・木村惣一・西原昌宏
    • Organizer
      植物細胞分子生物学会第28回大会・シンポジウム
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] リンドウから単離したMADS box遺伝子の機能解析2010

    • Author(s)
      齋藤美沙・中塚貴司・西原昌宏
    • Organizer
      植物細胞分子生物学会第28回大会・シンポジウム
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] リンドウ花器官形成に関与するMADS box遺伝子の解析2010

    • Author(s)
      中塚貴司、齋藤美沙、吉田恵理、西原昌宏
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-21
    • Related Report
      2010 Final Research Report
  • [Presentation] リンドウ花器官形成に関与するMADS box遺伝子の解析2010

    • Author(s)
      中塚貴司、齋藤美沙、吉田恵理、西原昌宏
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学黒髪北キャンパス
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.ibrc.or.jp/HPData2010/03Labo/03MolBreeding/FFHOME/FF_Homepage.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.ibrc.or.jp/HPData2010/03Labo/03MolBreeding/FFHOME/FF_Homepage.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.ibrc.or.jp/HPData2010/03Labo/03MolBreeding/FFHOME/FF_Homepage.html

    • Related Report
      2009 Annual Research Report

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Published: 2009-03-31   Modified: 2016-04-21  

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