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Genetic analysis of defense response against intracellular infection, induced by environmental stress response pathway.

Research Project

Project/Area Number 21780045
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied entomology
Research InstitutionIwate Medical University

Principal Investigator

TERASHIMA Jun  Iwate Medical University, 薬学部, 助教 (60400272)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords昆虫分子生物学 / 感染防御
Research Abstract

Ecdysone inducible protein 75B (E75B) is one of the ecdysone response genes and relates to metamorphosis in the insects. E75B has a role for nutritional shortage response, regulates the development of ovary in Drosophila under nutritional stress. Ecdysone is the insect steroid hormone and adjusts not only the metamorphosis but also stress response in Drosophila. We expected that steroid relate pathway has possibility to response against various stress in not only the insect but also mammalian cell, tried to apply the microarray analysis data to the investigation of stress response in human cancer, a human liver carcinoma cell line (HepG2). In this project, we show that E75B has important role for defense response against obligate intracellular parasite, Nosema bombycis in Drosophila melanogaster and low-glucose conditions induce the translocation of AhR into the nucleus in the human carcinoma cell line HepG2 and that AhR activates CYP1A1, CYP1A2, and CYP1B1 expression. The activation of CYP1 family member expression indicates that AhR binds to a ligand and successfully functions as a transcription factor. These results suggest that an endogenous AhR ligand is produced in HepG2 cells under low-glucose conditions and that AhR's role as a transcription factor is related to the low-glucose response of HepG2 cells.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (11 results)

All 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Remarks (4 results)

  • [Journal Article] Involvement of promoter methylation in the regulation of Pregnane X receptor in colon cancer cells.2011

    • Author(s)
      Habano W, Gamo T, Terashima J, Sugai T, Otsuka K, Wakabayashi G, Ozawa S.
    • Journal Title

      BMC Cancer. 11

      Pages: 81-90

    • NAID

      130004640031

    • Related Report
      2010 Final Research Report
  • [Journal Article] Cooperative regulation of the induction of the novel antibacterial listericin by PGRP-Le and the JAK-STAT pathway.2010

    • Author(s)
      Goto A, Yano T, Terashima J, Iwashita S, Oshima Y, Kurata S
    • Journal Title

      J Biol Chem 285

      Pages: 15731-15738

    • Related Report
      2010 Final Research Report
  • [Journal Article] Involvement of promoter methylation in the regulation of Pregnane X receptor in colon cancer cells.2010

    • Author(s)
      Habano W, Gamo T, Terashima J, Sugai T, Otsuka K, Wakabayashi G, Ozawa S.
    • Journal Title

      BMC Cancer.

      Volume: 11 Pages: 81-90

    • NAID

      130004640031

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cooperative regulation of the induction of the novel antibacterial listeric in by PGRP-Le and the JAK-STAT pathway.2010

    • Author(s)
      Goto A, Yano T, Terashima J, Iwashita S, Oshima Y, Kurata S
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 285 Pages: 15731-8

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cooperative regulation of the induction of the novel antibacterial listeric in by PGRP-Le and the JAK-STAT pathway.2010

    • Author(s)
      Goto A, Yana T, Terashima J, Iwashita S, Oshima Y, Kurata S.
    • Journal Title

      The Journal of Biological Chemistry Epub ahead of print

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Nutritional stress signaling induces CYP1A1 and CYP1B1 through AhR-mediated mechanisms.2010

    • Author(s)
      Terashima J., Habano W., Gamou T., Ozawa S.
    • Organizer
      日本薬物動態学会 第25回年会
    • Place of Presentation
      埼玉県さいたま市
    • Year and Date
      2010-10-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Nutritional stress signaling induces CYP1A1 and CYP1B1 through AhR-mediated mechanisms.2010

    • Author(s)
      Terashima J., Habano W., Gamou T., Ozawa S.
    • Organizer
      日本薬物動態学会 第25回年会
    • Place of Presentation
      さいたま市
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Remarks] マイクロアレイデータベース(東北大学)

    • URL

      http://www.pharm.tohoku.ac.jp/~seimei/m_array/index.htm

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.pharm.tohoku.ac.jp/~seimei/m_array/index.htm

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.pharm.tohoku.ac.jp/~seimei/m_array/index.htm

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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