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Basic studies on the insect cytochrome P450 for the development of new insecticides and synergistic agents.

Research Project

Project/Area Number 21780053
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied entomology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

KASAI Shinji  National Institute of Infectious Diseases, 昆虫医科学部, 主任研究官 (80332360)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords害虫管理 / 生物的防除 / シトクロムP450 / 共力剤 / 次世代の農薬 / ピレスロイド / 殺虫剤抵抗性 / デング熱 / kdr / ネッタイシマカ / ヒトスジシマカ / チクングニア / 局所施用 / ネッタイイエカ / ウエストナイル / 解毒酵素
Research Abstract

In order to seek novel target sites of insecticides or synergists, I studied on the mechanisms of insecticide resistance in vector mosquitoes, especially on the functional analysis of their cytochrome P450 monooxygenases. First of all, adult mosquitoes were selected in the laboratory with permethrin which is a class of pyrethroid insecticides. After the selections for 8 generations, the resistance level of mosquitoes (SPS8 strain) reached more than 1000-fold compared to an insecticide susceptible strain. Since permethrin susceptibility largely increased with pre-treatment of mosquitoes with piperonyl butoxide, it was suggested that cytochrome P450 monooxygenases have the major role in the resistance. In vitro metabolism study revealed that microsomal protein prepared from resistant mosquitoes catalyzed metabolism of ^<14>C-permethrin more rapidly to 4'HO-permethrin than that of a susceptible strain. Furthremore, permethrin hydroxylation activity was NADPH dependent and was strongly inhibited by piperonyl butoxide strongly suggested that cytochrome P450 monooxygenases are involved in permethrin resistance in SPS8 strain. The P450 isofoms which are associated with permethrin resistance are promising candidates as novel target sites of insecticides or synergists. Sodium channel gene was genotyped for 26 adult Aedes albopictus collected from Singapre in 2009. Surprisingly, more than 70% of mosquitoes possessed the knockdown resistance (kdr) gene. This is the first evidence for the presence of the kdr gene in Aedes albopictus and suggests the need to study the global distribution of this allele in this important vector insect.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report

Research Products

(28 results)

All 2011 2010 2009 Other

All Journal Article Presentation Book Remarks

  • [Journal Article] Cis-acting mutation and duplication: History of molecular evolution in a P450 haplotype responsible for insecticide resistance in Culex quinquefasciatus.2011

    • Author(s)
      Kentaro Itokawa, Osamu Komagata, Shinji Kasai, Masahiro Masada, Takashi Tomita
    • Journal Title

      Insect Biochemistry and Molecular Biology (in press)

    • Related Report
      2010 Final Research Report
  • [Journal Article] First detection of a putative knockdown resistance gene from the major mosquito vector, Aedes albopictus.2011

    • Author(s)
      Shinji Kasai, Lee Ching Ng, Sai Gek Lam-Phua, Choon Siang WTang, Kentaro Itokawa, Osamu Komagata, Mustuo Kobayashi, Takashi Tomita
    • Journal Title

      Japanese Journal of Infectious Diseases (in press)

    • NAID

      40018820936

    • Related Report
      2010 Final Research Report
  • [Journal Article] First detection of a putative knockdown resistance gene from the major mosquito vector, Aedes albopictus.2011

    • Author(s)
      Shinji Kasai, Lee Ching Ng, Sai Gek lam-Phua, Choon Siang Tang, Kentaro Itokawa, Osamu komagata, Mutsuo Kobayashi, Takashi Tomita
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 64(3)(印刷中)

    • NAID

      40018820936

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cis-acting mutation and duplication : History of molecular evolution in a P450 haplotype responsible for insecticide resistance in Culex quinquefasciatus.2011

    • Author(s)
      Kentaro Itokawa, Osamu Komagata, Shinji Kasai, Masahiro Masada, Takashi Tomita
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 41(印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomic structure of Cyp9m10 in a pyrethroid resistant and susceptible strains of Culex quinquefasciatus.2010

    • Author(s)
      Yoshika Okamura, Masahiro Masada Takashi Tomita
    • Journal Title

      Insect Biochemistry and Molecular Biology 40(9)

      Pages: 631-640

    • Related Report
      2010 Final Research Report
  • [Journal Article] hardstone, Osamu Komagata, Shinji Kasai, Takashi Tomita Jeffrey G. Scott Use of isogenic strains indicates CYP9M10 is linked to permethrin resistance in Culex pipiens quinquefasciatus.2010

    • Author(s)
      Melissa C.
    • Journal Title

      Insect Molecular Biology 19(6)

      Pages: 717-726

    • Related Report
      2010 Final Research Report
  • [Journal Article] 殺虫剤抵抗性蚊におけるシトクロムP450遺伝子の過剰発現2010

    • Author(s)
      冨田隆史, 糸川健太郎, 駒形修,葛西真治
    • Journal Title

      日本農薬学会誌 35(4)

      Pages: 562-568

    • NAID

      110008460402

    • Related Report
      2010 Final Research Report
  • [Journal Article] Genomic structure of Cyp9m10 in a pyrethroid resistant and susceptible strains of Culex quinquefasciatus.2010

    • Author(s)
      Kentaro Itokawa, Osamu Komagata, Shinji Kasai, Yoshika Okamura, Masahiro Masada, Takashi Tomita
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 40(9) Pages: 631-640

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Use of isogenic strains indicates CYP9M10 is linked to permethrin resistance in Culex pipiens quinquefasciatus.2010

    • Author(s)
      Melissa C.Hardstone, Osamu Komagata, Shinji Kasai, Takashi Tomita, Jeffrey G.Scott
    • Journal Title

      Insect Molecular Biology

      Volume: 19(6) Pages: 717-726

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 殺虫剤抵抗性蚊におけるシトクロムP450遺伝子の過剰発現2010

    • Author(s)
      冨田隆史, 糸川健太郎, 駒形修, 葛西真治
    • Journal Title

      日本農薬学会誌

      Volume: 35(4) Pages: 562-568

    • NAID

      110008460402

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of cytochrome P450 genes in pyrethroid resistant Culexquinquefasciatus.2010

    • Author(s)
      Osamu Komagata, Shinji Kasai, Takashi Tomita
    • Journal Title

      Insect Biochemistry and Molecular Biology 40

      Pages: 146-152

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alternative splicing and developmental regulation of glutathione transfe-rases in Culex quinquefasciatus Say.2009

    • Author(s)
      Shinji Kasai, Osamu Komagata, Yoshika Okamura, Takashi Tomita
    • Journal Title

      Pesticide Biochemistry and Physiology 94

      Pages: 21-29

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of cytochrome P450 genes in pyrethroid resistant Culex quinquefasciatus.

    • Author(s)
      Osamu Komagata, Shinji Kasai Takashi Tomita
    • Journal Title

      Insect Biochemistry and Molecular Biology 40(2)

      Pages: 146-152

    • Related Report
      2010 Final Research Report
  • [Presentation] Permethrin抵抗性ネッタイシマカの解毒と作用点変異2011

    • Author(s)
      葛西真治, 駒形修, 糸川健太郎, 小林睦生, 冨田隆史
    • Organizer
      日本農薬学会第36回大会
    • Place of Presentation
      玉川大学
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] ヒトスジシマカから初めて検出されたん4r遺伝子2010

    • Author(s)
      葛西真治, 駒形修, 小林睦生, 冨田隆史
    • Organizer
      第62回日本衛生動物学会東日本支部大会
    • Place of Presentation
      順天堂大学
    • Year and Date
      2010-10-16
    • Related Report
      2010 Final Research Report
  • [Presentation] ヒトスジシマカから初めて検出されたkdr遺伝子2010

    • Author(s)
      葛西真治, 駒形修, 小林睦生, 冨田隆史
    • Organizer
      第62回日本衛生動物学会東日本支部大会
    • Place of Presentation
      順天堂大学
    • Year and Date
      2010-10-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] ネソタイシマカ成虫のピレスロイド剤抵抗性機構(1)2010

    • Author(s)
      葛西真治, 駒形修, 糸川健太郎, 小林睦生, 冨田隆史
    • Organizer
      第62回日本衛生動物学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2010-04-03
    • Related Report
      2010 Final Research Report
  • [Presentation] アカイエカ種群蚊のピレスロイド抵抗性遺伝子媚rの遺伝子型2010

    • Author(s)
      駒形修, 葛西真治, 糸川健太郎, 二瓶直子, 津田良夫, 小林睦生, 冨田隆史
    • Organizer
      第62回日本衛生動物学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2010-04-03
    • Related Report
      2010 Final Research Report
  • [Presentation] ネッタイシマカ成虫のピレスロイド剤抵抗性機構(1)2010

    • Author(s)
      葛西真治, 駒形修, 糸川健太郎, 小林睦生, 冨田隆史
    • Organizer
      第62回日本衛生動物学会大会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2010-04-03
    • Related Report
      2010 Annual Research Report 2009 Annual Research Report
  • [Presentation] 殺虫剤抵抗性と連鎖するCyPgM10遺伝子の過剰発現と進化2010

    • Author(s)
      糸川健太郎, 駒形修, 葛西真治, 川田均, 岡村佳香, 政田正弘, 冨田隆史
    • Organizer
      第53回日本応用動物昆虫学会大会
    • Place of Presentation
      千葉
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] ネッタイイエカ成虫のピレスロイド剤抵抗性機構2010

    • Author(s)
      駒形修, 葛西真治, 糸川健太郎, 冨田隆史
    • Organizer
      第53回日本応用動物昆虫学会大会
    • Place of Presentation
      千葉
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] ネッタイイエカ成虫のピレスロイド剤抵抗性機構(1)2010

    • Author(s)
      葛西真治、駒形修、糸川健太郎、小林睦生、冨田隆史
    • Organizer
      第54回日本応用動物昆虫学会
    • Place of Presentation
      千葉大学
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] ネッタイイエカ幼虫のピレスロイド抵抗性に関与する解毒酵素シトクロムP450(CYPgM10)の遺伝学的研究2009

    • Author(s)
      糸川健太郎, 駒形修, 葛西真治, 政田正弘, 冨田隆史
    • Organizer
      第61回日本衛生動物学会東日本支部大会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-17
    • Related Report
      2010 Final Research Report
  • [Book] 第7章,環境適応,分子昆虫学-ポストゲノムの昆虫研究-(神村学,日本典秀,葛西真治,竹内秀明,畠山正統,石橋純編)東京2009

    • Author(s)
      葛西真治
    • Publisher
      共立出版
    • Related Report
      2010 Final Research Report
  • [Book] Advances in Human Vector Control2009

    • Author(s)
      Osamu Komagata, Shinji Kasai, Takashi Tomita
    • Total Pages
      241
    • Publisher
      Insecticide resistance in the mosquito Culex pipiens complex : concerns about development of pyrethroid ressistance.
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.nih.go.jp/niid/entomology/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.nih.go.jp/niid/entomology/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.nih.go.jp/niid/entomology/

    • Related Report
      2009 Annual Research Report

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Published: 2009-03-31   Modified: 2016-04-21  

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