Identification of Rhizobial secreted proteins which are responsible for the nodulation on Rj soybean

• Project/Area Number: 21780056
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant nutrition/Soil science
• Research Institution: Nara Women's University
• Principal Investigator: OKAZAKI Shin 奈良女子大学, 大学院・人間文化研究科, 助教 (40379285)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 根粒菌 / 共生窒素固定 / ダイズ / III 型分泌機構 / マメ科植物 / III型分泌機構 / 共生 / 防御応答 / シグナル伝達 / Rj遺伝子

Research Abstract

rj_1 and nfr1 mutant soybeans formed nodules by rhizobia harboring the type III secretion system (T3SS). Host nodulation genes were induced in the root inoculated with the T3SS-harboring rhizobia, suggesting that T3SS enforces legume host to initiate symbiotic programs.

Research Products

• [Journal Article] Identification and functional analysis of Type III effector proteins in Mesorhizobium loti (2010)
  • Author(s): Okazaki S, Okabe S, Higashi M, Shimoda Y, Sato S, Tabata S, Hashiguchi M, Akashi R, Gottfert M, K. Saeki
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 23 Pages: 223-342
  • Peer Reviewed
• [Journal Article] Identification and Functional Analysis of Type III Effector Proteins in Mesorhizobium loti (2010)
  • Author(s): 岡崎伸
  • Journal Title: Molecular Plant-Microbe Interactions Volume: Vol.23 Pages: 223-234
  • Peer Reviewed
• [Journal Article] Genetic organization and functional analysis of the type III secretion system of Bradyrhizobium elkanii (2009)
  • Author(s): Okazaki S, Zehner S, Hempel J, Lang K, Gottfert M
  • Journal Title: FEMS Microbiology Letters Volume: 95 Pages: 88-95
  • Peer Reviewed
• [Journal Article] Genetic organization and functional analysis of the type III secretion system of Bradyrhizobium elkanii (2009)
  • Author(s): 岡崎伸
  • Journal Title: FEMS Microbiology Letters Volume: Vol.295 Pages: 88-95
  • Peer Reviewed
• [Presentation] 根粒菌のIII 型分泌機構はマメ科植物の共生シグナルを活性化する (2011)
  • Author(s): 岡崎伸
  • Organizer: 第５回日本ゲノム微生物学会若手の会
  • Place of Presentation: 静岡
  • Year and Date: 2011-09-29
• [Presentation] 根粒菌III 型分泌系によるマメ科植物共生シグナルの活性化機構 (2011)
  • Author(s): 岡崎伸, 佐伯和彦
  • Organizer: 植物微生物研究会第２１回研究交流会
  • Place of Presentation: 岡山
  • Year and Date: 2011-09-22
• [Presentation] マメ科植物と共生する根粒菌のIII 型分泌機構 (2011)
  • Author(s): 岡崎伸
  • Organizer: 第５回細菌学若手コロッセウム
  • Place of Presentation: 高知
  • Year and Date: 2011-08-09
• [Presentation] Rhizobial Type III Secretion System Modulates the Nodulation Signaling in Soybean (2010)
  • Author(s): Shin Okazaki
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Aoshima Palmbeach Hotel
  • Year and Date: 2010-11-21
• [Presentation] マメ科植物と共生する根粒菌のIII 型分泌機構 (2010)
  • Author(s): 岡崎伸, 東未来, 岡部沙織, 佐伯和彦
  • Organizer: 第４回ゲノム微生物学会 若手の会
  • Place of Presentation: 神戸
  • Year and Date: 2010-10-01
• [Presentation] マメ科植物と共生する根粒菌のIII型分泌機構 (2010)
  • Author(s): 岡崎伸
  • Organizer: 第4回ゲノム微生物学会若手の会
  • Place of Presentation: 神戸セミナーハウス
  • Year and Date: 2010-10-01
• [Presentation] Rhizobial Type III Secretion System Modulates the Nodulation Signaling in Soybean (2010)
  • Author(s): Okazaki S, Saeki K
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: 宮崎
  • Year and Date: 2010-09-21
• [Presentation] マメ科植物との共生を決定づけるミヤコグサ根粒菌３型分泌系 (2010)
  • Author(s): 岡崎伸, 東未来, 岡部沙織, 佐伯和彦
  • Organizer: 日本植物生理学会第51回年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-19
• [Presentation] マメ科植物との共生を決定づけるミヤコグサ根粒菌3型分泌系 (2010)
  • Author(s): 岡崎伸
  • Organizer: 日本植物生理学会第51回年会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 2010-03-18
• [Presentation] Symbiotic Roles of Rhizobial Type III Secretion Systems (2010)
  • Author(s): Okazaki S, S Okabe, M Higashi, Gottfert M, K Saeki
  • Organizer: The 7th Okazaki Biology Conference
  • Place of Presentation: 掛川
  • Year and Date: 2010-01-14
• [Presentation] Symbiotic Roles of Rhizobial Type III Secretion Systems (2010)
  • Author(s): 岡崎伸
  • Organizer: The 7th Okazaki Biology Conference
  • Place of Presentation: ヤマハリゾート嬬恋(掛川)
  • Year and Date: 2010-01-11
• [Presentation] マメ科植物との共生における根粒菌３型分泌系の役割 (2009)
  • Author(s): 岡崎伸, 東未来, 岡部沙織, 佐伯和彦
  • Organizer: 第３回細菌学若手コロッセウム
  • Place of Presentation: 宮崎
  • Year and Date: 2009-10-27
• [Presentation] マメ科植物との共生における根粒菌3型分泌系の役割 (2009)
  • Author(s): 岡崎伸
  • Organizer: 第3回細菌学・若手コロセウム
  • Place of Presentation: サンホテルフェニックス(宮崎)
  • Year and Date: 2009-10-26
• [Presentation] Rj 遺伝子保有ダイズとの共生を司る根粒菌Type III 分泌系植物微生物研究会第19 回研究交流会 (2009)
  • Author(s): 岡崎伸, 佐伯和彦
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: 松本
  • Year and Date: 2009-09-09
• [Presentation] Rj遺伝子保有ダイズとの共生を司る根粒菌TypeIII分泌系 (2009)
  • Author(s): 岡崎伸
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: あがたの森文化会館(松本)
  • Year and Date: 2009-09-08
• [Presentation] 宿主特異性を決定づけるミヤコグサ根粒菌Type III エフェクターの同定 (2009)
  • Author(s): 岡崎伸, 東未来, 岡部沙織, 佐伯和彦
  • Organizer: 第56回日本生化学会近畿支部例会
  • Place of Presentation: 高槻
  • Year and Date: 2009-07-25
• [Presentation] Identification and functional analysis of type III effector proteins in Mesorhizobium loti (2009)
  • Author(s): Okazaki S, M Higashi, S Okabe, Gottfert M, K Saeki
  • Organizer: 14th International congress on molecular plant-microbe interactions
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2009-07-21
• [Presentation] Identification and functional analysis of type III effector proteins in Mesorhizobium loti (2009)
  • Author(s): 岡崎伸
  • Organizer: 14th International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: Centre de Congres(カナダ、ケベック)
  • Year and Date: 2009-07-19
• [Presentation] 宿主特異性を決定づけるミヤコグサ根粒菌Type IIIエフェクターの同定 (2009)
  • Author(s): 岡崎伸
  • Organizer: 第56回日本生化学会近畿支部例会
  • Place of Presentation: 大阪医科大学(高槻)
  • Year and Date: 2009-07-18