• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Optimization of technologies for improving microbial metabolisms using antisense RNA expression system

Research Project

Project/Area Number 21780071
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionOsaka University

Principal Investigator

HIRASAWA Takashi  Osaka University, 大学院・情報科学研究科, 助教 (20407125)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
KeywordsアンチセンスRNA / コリネ型細菌 / グルタミン酸 / 2-オキソグルタル酸デヒドロゲナーゼ複合体 / odhA / OdhA
Research Abstract

In this study, effectiveness of antisense RNA expression system was examined using antisense RNA expression system for odhA mRNA of a coryneform bacterium Corynebacterium glutamicum. Moreover, effective design of antisense RNA for improving metabolisms and optimization of antisense RNA expression system were tried by constructing the antisense RNAs with various length and those based on the secondary structure of target mRNA.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (39 results)

All 2011 2010 2009 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (23 results) Remarks (2 results)

  • [Journal Article] Investigation of phosphorylation status of OdhI protein during penicillin- and Tween 40-triggered glutamate overproduction by Oorynebacterium glutamioum.2011

    • Author(s)
      Jongpill Kim, Takashi Hirasawa, Masaki Saito, Chikara Furusawa, Hiroshi Shimizu.
    • Journal Title

      Appl.Microbiol.Biotechnol. In press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive phenotypic analysis of single-gene deletion and overexpression strains of Saocharomyoes cerevisiae.2011

    • Author(s)
      Katsunori Yoshikawa, Tadamasa Tanaka, Yoshihiro Ida, Chikara Furusawa, Takashi Hirasawa, Hiroshi Shimizu.
    • Journal Title

      Yeast 28

      Pages: 349-361

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Saecharomyces oerevisiae and DNA microarray analyses : what did we learn from it for a better understanding and exploitation of yeast biotechnology?2010

    • Author(s)
      Takashi Hirasawa, Chikara Furusawa, Hiroshi Shimizu.
    • Journal Title

      Appl.Microbiol.Biotechnol. 87

      Pages: 391-400

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Requirement of de novo synthesis of the OdhI protein in penicillin-induced glutamate production by COTynebaeterium glutamicum.2010

    • Author(s)
      Jongpill Kim, Hirohisa Fukuda, Takashi Hirasawa, Keisuke Nagahisa, Kazuo Nagai, Masaaki Wachi, Hiroshi Shimizu.
    • Journal Title

      Appl.Microbiol.Biotechnol. 86

      Pages: 911-920

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptome analysis of parallel-evolved Escherichia coli strains under ethanol stress2010

    • Author(s)
      Takaaki Horinouchi, Takashi Hirasawa
    • Journal Title

      BMC Genomics

      Pages: 579-579

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide expression analysis of Saccharomyces pastorianus orthologous genes using oligonucleotide microarrays2010

    • Author(s)
      Takaaki Horinouchi, Takashi Hirasawa
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 110 Pages: 602-607

    • NAID

      110007988814

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Saccharomyces cerevisiae and DNA microarray analyses : what did we learn from it for a better understanding and exploitation of yeast biotechnology2010

    • Author(s)
      Takashi Hirasawa
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 87 Pages: 391-400

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential importance of trehalose accumulation in Saccharomyces cerevisiae in response to various environmental stresses2010

    • Author(s)
      Siraje Arif Mahmud
    • Journal Title

      Journal of Bioscience and Bioengineering 109

      Pages: 262-266

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Requirement of de novo synthesis of the OdhI protein in penicillin-induced glutamate production by Corynebacterium glutamicum2010

    • Author(s)
      Jongpill Kim
    • Journal Title

      Applied Microbiology and Biotechnology (未定)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential importance of trehalose accumulation in SaochaTomyoes ceTeyisiae in response to various environmental stresses.2009

    • Author(s)
      Siraje Arif Mahmud, Takashi Hirasawa, Hiroshi Shimizu.
    • Journal Title

      J.Biosci.Bioeng. 109

      Pages: 262-266

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development and experimental verification of a genome-scale metabolic model for Corynebacterium glutamicum.2009

    • Author(s)
      Yohei Shinfuku, Natee Sorpitiporn, Masahiro Sono, Chikara Furusawa, Takashi Hirasawa, Hiroshi Shimizu.
    • Journal Title

      Microb.CellFact. 8

      Pages: 43-43

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development and experimental verification of a genome-scale metabolic model for Corynebacterium glutamicum2009

    • Author(s)
      Yohei Shinfuku
    • Journal Title

      Microbial Cell Factories 8

      Pages: 43-43

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of adaptation to high ethanol concentration in Saccharomyces cerevisiae using DNA microarray2009

    • Author(s)
      Thai Nho Dinh
    • Journal Title

      Bioprocess and Biosystems Engineering 32

      Pages: 681-688

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Investigating the effectiveness of DNA microarray analysis for identifying the genes involved in L-lactate production by Saccharomyces cerevisiae2009

    • Author(s)
      Takashi Hirasawa
    • Journal Title

      Applied Microbiology and Biotechnology 84

      Pages: 1149-1159

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] コリネ型細菌によるグルタミン酸生産におけるOdhIタンパク質の役割2011

    • Author(s)
      平沢敬、金鍾弼、古澤力, 清水浩
    • Organizer
      日本農芸化学会大会2011年度大会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] コリネ型細菌によるグルタミン酸生産におけるOdhIタンパク質の役割2011

    • Author(s)
      平沢敬
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学(京都府)
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 異種タンパク質を分泌生産するコリネ型細菌の代謝フラックス解析2010

    • Author(s)
      馬越元基、平沢敬、古澤力、竹中康浩、菊池慶実、清水浩
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 異種タンパク質を分泌生産するコリネ型細菌の代謝フラックス解析2010

    • Author(s)
      馬越元基、平沢敬
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      宮崎シーガイアワールドコンベンションセンターサミット(宮崎県)
    • Year and Date
      2010-10-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸菌のゲノムスケール代謝モデルによる有用物質生産に向けた代謝予測2010

    • Author(s)
      大野聡、古澤力、田中章太郎、平沢敬、清水浩
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Corynebacterium glutamicumによるグルタミン酸生産におけるOdhIタンパク質のリン酸化状態の解析2010

    • Author(s)
      平沢敬、金鍾弼、古澤力、清水浩
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Corynebacterium glutamicumによるグルタミン酸生産におけるOdhIタンパク質のリン酸化状態の解析2010

    • Author(s)
      平沢敬
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      宮崎シーガイアワールドコンベンションセンターサミット(宮崎県)
    • Year and Date
      2010-10-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] ゲノムスケール代謝モデルによる代謝フラックス予測とその実験的検証2010

    • Author(s)
      古澤力、平沢敬、小野直亮、清水浩
    • Organizer
      第62回日本生物工学会大会・大学発技術シーズ発表会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-27
    • Related Report
      2010 Final Research Report
  • [Presentation] ゲノムスケール代謝モデルによる代謝フラックス予測とその実験的検証2010

    • Author(s)
      古澤力、平沢敬
    • Organizer
      第62回日本生物工学会大会
    • Place of Presentation
      宮崎シーガイアワールドコンベンションセンターサミット(宮崎県)
    • Year and Date
      2010-10-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] プロテオミクスによるコリネ型細菌グルタミン酸生産メカニズム解析と生産性向上2010

    • Author(s)
      Kim Jongpill、福田洋久、平沢敬、永久圭介、古澤力、清水浩
    • Organizer
      化学工学会第42回秋季大会
    • Place of Presentation
      京都
    • Year and Date
      2010-09-08
    • Related Report
      2010 Final Research Report
  • [Presentation] プロテオミクスによるコリネ型細菌グルタミン酸生産メカニズム解析と生産性向上2010

    • Author(s)
      Jongpill Kim、平沢敬
    • Organizer
      化学工学会第42回秋季大会
    • Place of Presentation
      同志社大学今出川キャンパス(京都府)
    • Year and Date
      2010-09-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸菌の中央代謝系酵素遺伝子発現調節株の構築-遺伝子摂動が代謝におよぼす影響の解析に向けて2010

    • Author(s)
      平沢敬、臼井佑希、古澤力、山本奈津子、森浩禎、清水浩
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2010-03-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 大腸菌の中央代謝系酵素遺伝子発現調節株の構築-遺伝子摂動が代謝におよぼす影響の解析に向けて2010

    • Author(s)
      平沢敬
    • Organizer
      第4回日本ゲノム微生物学会年会
    • Place of Presentation
      九州大学
    • Year and Date
      2010-03-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大腸菌の細胞内代謝に対する遺伝子摂動の影響の解析2009

    • Author(s)
      臼井佑希、平沢敬、古澤力、山本奈津子、森浩禎、清水浩
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] 酵母を用いたL-乳酸生産に関連する遺伝子のゲノムワイドな同定2009

    • Author(s)
      平沢敬、大久保亜紀、竹國正矩、吉川勝徳、古澤力、清水浩
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 酵母を用いたL-乳酸生産に関連する遺伝子のゲノムワイドな同定2009

    • Author(s)
      平沢敬
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Flux balance analysis of Corynebacterium glutamicum using a genome-scale metabolic model2009

    • Author(s)
      Chikara Furusawa
    • Organizer
      Asia Pacific Biochemical Engineering Conference (APBioChEC' 09)
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-11-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 異種タンパク質を分泌生産するコリネ型細菌の代謝解析2009

    • Author(s)
      馬越元基、平沢敬、古澤力、竹中康浩、菊池慶実、清水浩
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] ゲノムスケール代謝モデルを用いたCorynebacterium glutamicumの代謝解析とその実験的検証2009

    • Author(s)
      新福洋平、ソーピティポーンナティー、園雅博、古澤力、平沢敬、清水浩
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] ゲノムスケール代謝モデルを用いたCorynebacterium glutamicumの代謝解析とその実験的検証2009

    • Author(s)
      古澤力
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 異種タンパク質を分泌生産するコリネ型細菌の代謝解析2009

    • Author(s)
      馬越元基
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] ゲノム情報を基盤とする代謝予測システムの開発と実験検証2009

    • Author(s)
      新福洋平、Sorpitiporn Natee、園雅博、平沢敬、古澤力、清水浩
    • Organizer
      化学工学会第41回秋季大会
    • Place of Presentation
      広島
    • Year and Date
      2009-09-16
    • Related Report
      2010 Final Research Report
  • [Presentation] ゲノム情報を基盤とする代謝予測システムの開発と実験検証2009

    • Author(s)
      清水浩
    • Organizer
      化学工学会第41回秋季大会
    • Place of Presentation
      広島大学
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www-shimizu.ist.osaka-u.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www-shimizu.ist.osaka-u.ac.jp

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi