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Analysis of a highly stable cholesterol oxidase from a gram-negative bacterium

Research Project

Project/Area Number 21780082
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionToyo University

Principal Investigator

DOUKYU Noriyuki  東洋大学, 生命科学部, 教授 (60302957)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsコレステロール / コレステロールオキシダーゼ / 臨床検査 / Chromobacterium / グラム陰性細菌
Research Abstract

Cholesterol oxidase is a valuable enzyme which is used for determination of cholesterol in human blood. We previously discovered a thermal, organic solvent and detergent-tolerant cholesterol oxidase from a gram-negative bacterium Chromobacterium sp. DS-1.In this study, the mechanism of the high stability of the enzyme was studied. As the result, it was found that a coenzyme FAD is involved in the stability of the enzyme.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (31 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 8 results) Presentation (20 results) Remarks (1 results)

  • [Journal Article] Novel cholesterol oxidases which were found in the studies of applications of organic solvent-tolerant bacteria2012

    • Author(s)
      Noriyuki Doukyu
    • Journal Title

      J.Jpn.Soc.Extremophiles

      Volume: 10 Pages: 13-16

    • NAID

      40019582650

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Novel cholesterol oxidases which were found in the studies of applications of organic solvent-tolerant bacteria2011

    • Author(s)
      N. Doukyu
    • Journal Title

      J. Jpn. Soc. Extremophiles

      Volume: 10 Pages: 13-16

    • NAID

      40019582650

    • Related Report
      2011 Final Research Report
  • [Journal Article] Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sp2010

    • Author(s)
      M. Sagermann, A. Ohtaki, K. Newton, N. Doukyu
    • Journal Title

      J. Struct. Biol

      Volume: 170 Pages: 32-40

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Organic solvent-tolerant enzymes2010

    • Author(s)
      N. Doukyu and H. Ogino
    • Journal Title

      Biochem. Eng. J

      Volume: 48 Pages: 270-282

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sn.DS-1.2010

    • Author(s)
      Sagermann, M., Ohtaki, A., Newton, K., Doukyu, N.
    • Journal Title

      J.Struct.Biol.

      Volume: 170 Pages: 32-40

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organic solvent-tolerant enzymes.2010

    • Author(s)
      Doukyu, N., Ogino, H.
    • Journal Title

      Biochem.Eng.J.

      Volume: 48 Pages: 270-282

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural characterization of the organic solvent-stable cholesterol oxidase from Chromobacterium sp. DS-1.2010

    • Author(s)
      Sagermann, M., Ohtaki, A., Newton, K., Doukyu, N.
    • Journal Title

      J.Struct.Biol. 170

      Pages: 32-40

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organic solvent-tolerant enzymes.2010

    • Author(s)
      Doukyu, N., Ogino, H.
    • Journal Title

      Biochem.Eng.J. 48

      Pages: 270-282

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics and biotechnological applications of microbial cholesterol oxidases2009

    • Author(s)
      N. Doukyu
    • Journal Title

      Appl. Microbiol. Biotechnol

      Volume: 83 Pages: 825-837

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Charactedstics and biotechnological applications of microbial cholesterol oxidases.2009

    • Author(s)
      Doukyu, N.
    • Journal Title

      Appl.Microbiol.Biotechnol. 83

      Pages: 825-837

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Novel microbial cholesterol oxidases-useful enzymes for multiple applications2011

    • Author(s)
      N. Doukyu
    • Organizer
      9^<th> International Symposium on Bioscience and Nanotechnology
    • Place of Presentation
      Kawagoe, Japan
    • Year and Date
      2011-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of cholesterol oxidases from gram negative bacteria2011

    • Author(s)
      S. Nihei, N. Ono, N. Doukyu, M. Sagermann
    • Organizer
      9^<th> International Symposium on Bioscience and Nanotechnology
    • Place of Presentation
      Kawagoe, Japan
    • Year and Date
      2011-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 組換え大腸菌によるPseudomonas aeruginosa由来コレステロールオキシダーゼの生産2011

    • Author(s)
      二瓶翔、道久則之
    • Organizer
      極限環境微生物学会
    • Place of Presentation
      長崎大学(長崎県)
    • Year and Date
      2011-11-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Chromobacterium.sp DS-1株由来コレステロールオキシダーゼの触媒部位の解析2011

    • Author(s)
      小野奈津美、道久則之
    • Organizer
      極限環境微生物学会
    • Place of Presentation
      長崎大学(長崎県)
    • Year and Date
      2011-11-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Novel cholesterol oxidases from bacteria-Useful enzymes for multiple applications2011

    • Author(s)
      N. Doukyu
    • Organizer
      BNERC. IIT Delhi Seminar, International Seminar for the 125^<th> Anniversary of Toyo University
    • Place of Presentation
      India, Delhi
    • Year and Date
      2011-08-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 組換え大腸菌によるPseudomonas aeruginosa由来コレステロールオキシダーゼの生産2011

    • Author(s)
      二瓶翔、道久則之
    • Organizer
      極限環境微生物学会2011年度年会
    • Place of Presentation
      長崎大学
    • Related Report
      2011 Final Research Report
  • [Presentation] Chromobacterium. sp DS-1株由来コレステロールオキシダーゼの触媒部位の解析2011

    • Author(s)
      小野奈津美、道久則之
    • Organizer
      極限環境微生物学会2011年度年会
    • Place of Presentation
      長崎大学
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of highly stable cholesterol oxidases from gram negative bacteria2010

    • Author(s)
      N. Ono, S. Nihei, N. Doukyu, M. Sagermann
    • Organizer
      8^<th> International Symposium on Bioscience and Nanotechnology
    • Place of Presentation
      Tokyo
    • Year and Date
      2010-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] 臨床検査に役立つ酵素~細菌のコレステロールオキシダーゼの研究~2010

    • Author(s)
      道久則之
    • Organizer
      東洋大学生命科学部シンポジウム
    • Place of Presentation
      東洋大学板倉校舎
    • Year and Date
      2010-11-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 有機溶媒耐性菌の応用研究から見出された新規なコレステロール酸化酵素に関する研究2010

    • Author(s)
      道久則之
    • Organizer
      極限環境微生物学会2010年度年会、極限環境生物学会・研究奨励賞講演
    • Place of Presentation
      京都大学宇治キャンパス
    • Year and Date
      2010-11-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 有機溶媒耐性菌の応用研究から見出された新規なコレステロール酸化酵素に関する研究2010

    • Author(s)
      道久則之
    • Organizer
      極限環境微生物学会 第11回年会
    • Place of Presentation
      京都(京都大学)
    • Year and Date
      2010-11-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] コレステロール値を測定するための安定なコレステロールオキシダーゼ2010

    • Author(s)
      道久則之
    • Organizer
      大学連携新技術説明会
    • Place of Presentation
      JSTホール東京
    • Year and Date
      2010-10-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 熱、有機溶媒、界面活性剤耐性のコレステロールオキシダーゼの解析2010

    • Author(s)
      道久則之
    • Organizer
      CPhI Japan, TLO/大学知的財産本部セミナー
    • Place of Presentation
      東京ビッグサイト
    • Year and Date
      2010-04-23
    • Related Report
      2011 Final Research Report
  • [Presentation] Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニングと発現2010

    • Author(s)
      道久則之、柴田貫平
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニングと発現2010

    • Author(s)
      道久則之、柴田貫平
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京(東京大学)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cholesterol oxidases from organic solvent-tolerant bacteria2010

    • Author(s)
      N. Doukyu
    • Organizer
      The 8th International Congress on Extremophiles
    • Place of Presentation
      Ponta Delgada. Portugal
    • Related Report
      2011 Final Research Report
  • [Presentation] Cholesterol oxidases from organic solvent-tolerant bacteria.2010

    • Author(s)
      道久則之
    • Organizer
      The 8th International Congress on Extremophiles
    • Place of Presentation
      Ponta Delgada, Portugal
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cloning, sequence analysis and expression of a gene encoding Chromobacterium sp. DS-1 cholesterol oxidase2009

    • Author(s)
      N. Doukyu, K. Shibata, M. Sagermann
    • Organizer
      7^<th> International Symposium on Bioscience and Nanotechnology
    • Place of Presentation
      Toyo University
    • Year and Date
      2009-11-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニング2009

    • Author(s)
      道久則之
    • Organizer
      極限環境微生物学会第10回年会
    • Place of Presentation
      明治大学
    • Year and Date
      2009-10-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Chromobacterium属細菌由来コレステロールオキシダーゼ遺伝子のクローニング2009

    • Author(s)
      道久則之
    • Organizer
      極限環境微生物学会第10回年会
    • Place of Presentation
      東京(明治大学)
    • Year and Date
      2009-10-29
    • Related Report
      2009 Annual Research Report
  • [Remarks] 東洋大学研究者情報データベース

    • URL

      http://ris.toyo.ac.jp/details/index.php?user_id=791

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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