Analysis of high-temperature tolerance mechanism and exploration of functional molecule(s) in heat response of higher plant

• Project/Area Number: 21780087
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Yoshihiro Tohoku University, 大学院・生命科学研究科, 准教授 (20390891)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 植物生化学 / ポリアミン / スペルミン / 高温ストレス / ポリアミン酸化酵素 / サーモスペルミン

Research Abstract

Polyamine is important for various physiological processes as well as several environmental stress responses in plant. In this study, I demonstrated that one of the polyamine, spermine, is involved in high-temperature tolerance in Arabidopsis thaliana. Moreover, I established a quantification method of the two tetraamine, spermine and thermospermine, and also performed characterization of polyamine oxidases in Arabidopsis.

Research Products

• [Journal Article] Characterization of five polyamine oxidase isoforms in Arabidopsis thaliana. (2010)
  • Author(s): Takahashi, Y., Cong, R., Sagor, G.H.M., Niitsu, M., Berberich, T. Kusano, T.
  • Journal Title: Plant Cell Rep. 29 Pages: 955-965
  • Peer Reviewed
• [Journal Article] Quantitative analysis of plant polyamines including thermospermine during growth and salinity stress. (2010)
  • Author(s): Naka, Y., Watanabe, K., Sagor, G.H.M., Niitsu, M., Pillai, M.A., Kusano, T., Takahashi, Y.
  • Journal Title: Plant Physiol. Biochem. 48 Pages: 527-533
  • Peer Reviewed
• [Journal Article] Characterization of five polyamine oxidase isoforms in Arabidopsis thaliana (2010)
  • Author(s): Takahashi, et al
  • Journal Title: Plant Cell Rep. Volume: 29 Pages: 955-965
  • Peer Reviewed
• [Journal Article] Quantitative analysis of plant polyamines including thermospermine during growth and salinity stress (2010)
  • Author(s): Naka, et al.
  • Journal Title: Plant Physiology and Biochemistry (印刷中)
  • Peer Reviewed
• [Presentation] シロイヌナズナにおける熱ショックストレスに対するスペルミンの防御的役割 (2011)
  • Author(s): Sagor, G.H.M., ら
  • Organizer: 日本ポリアミン学会
  • Place of Presentation: 帝京大学
• [Presentation] シロイヌナズナにおける熱ショックストレスに対するスペルミンの防御的役割 (2010)
  • Author(s): Sagor GHM, 新津勝, 草野友延, 高橋芳弘
  • Organizer: 日本ポリアミン学会第2回年会
  • Place of Presentation: 帝京大学
• [Presentation] Molecular biological and biochemical characterization of five polyamine oxidase isoforms in Arabidopsis thaliana. (2010)
  • Author(s): Takahashi, Y., Cong, R., Sagor, G.H.M., Niitsu, M., Berberich, T., Kusano, T.
  • Organizer: 2010国際ポリアミン会議
  • Place of Presentation: 静岡県御殿場高原リゾート時之栖
• [Presentation] サーモスペルミンの植物からの定量的検出法 (2010)
  • Author(s): 渡邉佳奈子, 仲友紀恵, Sagor GHM, 新津勝, Pillai MA, 草野友延, 高橋芳弘
  • Organizer: 日本農芸化学会2010年度(平成22年度)大会
  • Place of Presentation: 東京大学
• [Presentation] Molecular biological and biochemical characterization of five polyamine oxidase isoforms in Arabidopsis thariana (2010)
  • Author(s): Takahashi, ら
  • Organizer: 2010国際ポリアミン会議
  • Place of Presentation: 静岡県御殿場高原リゾート時之栖
• [Presentation] 二つのテトラアミン異性体であるスペルミンとサーモスペルミンのHPLCによる定量分析法の確立 (2009)
  • Author(s): 仲友紀恵, 渡邉佳奈子, Sagor GHM, 新津勝, Pillai MA, 草野友延, 高橋芳弘
  • Organizer: 植物化学調節学会第44回大会
  • Place of Presentation: 東北大学
• [Presentation] 二つのテトラアミン異性体であるスペルミンとサーモスペルミンのHPLCによる定量分析手法の確立 (2009)
  • Author(s): 仲友紀恵, ら
  • Organizer: 植物化学調節学会第44回大会
  • Place of Presentation: 東北大学
• [Remarks] ホームページ等
  • URL: http://www.ige.tohoku.ac.jp/outou/index-j.html