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Functional Analysis of RNA helicases in regulation of miRNA processing

Research Project

Project/Area Number 21780091
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionThe University of Tokyo

Principal Investigator

FUJIYAMA Sally  The University of Tokyo, 分子細胞生物学研究所, 助教 (50447372)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords遺伝子発現 / マイクロRNA / RNAヘリカーゼ / ショウジョウバエ遺伝学
Research Abstract

RNA helicase are enzymes to modulate the structures of double strand RNA and RNA-protein and are classified into families by their functional domains. There are several hundred kinds of putative RNA helicases, however, functions of only a little kind of helicases are elucidated. Thus, in this study I try to clarify the biological functions and reaction mechanisms of the novel RNA helicases by combination of Drosophila genetics and proteomic analysis.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report

Research Products

(35 results)

All 2011 2010 2009 Other

All Journal Article Presentation Book Remarks

  • [Journal Article] A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing.2011

    • Author(s)
      Ishiguro K, Kim J, Fujiyama-Nakamura S, Kato S, Watanabe Y
    • Journal Title

      EMBO Rep. vol.12

      Pages: 267-275

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing.2011

    • Author(s)
      Ishiguro K, Kim J, Fujiyama-Nakamura S, Kato S, Watanabe Y
    • Journal Title

      EMBO Rep.

      Volume: 12 Pages: 267-275

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of osteoclastic factors in the nuclear envelope of mature, multinucleated osteoclasts.2010

    • Author(s)
      Youn MY, Fujiyama-Nakamura S, Takada I, Imai Y, Kato S.
    • Journal Title

      Biosci Biotechnol Biochem. vol.74

      Pages: 1956-1959

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor.2010

    • Author(s)
      Sawatsubashi S, Murata T, Lim J, Fujiki R, Ito S, Suzuki E, Tanabe M, Zhao Y, Kimura S, Fujiyama S, Ueda T, Umetsu D, Ito T, Takeyama K, Kato S.
    • Journal Title

      Genes Dev. vol.24

      Pages: 159-170

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and identification of estrogen receptor alpha co-regulators in osteoclasts.2010

    • Author(s)
      Takada I, Tsuji N, Youn MY, Fujiyama S, Okada M, Imai Y, Kondo S, Kitakawa H, Yasuda H, Kato S.
    • Journal Title

      Ann NY Acad.Sci. vol.1192

      Pages: 201-207

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The metazoan ATAC and SAGA coactivator HAT complexes regulate different sets of inducible target genes.2010

    • Author(s)
      Nagy Z, Riss A, Fujiyama S, Krebs A, Orpinell M, Jansen P, Cohen A, Stunnenberg HG, Kato S, Tora L.
    • Journal Title

      Cell Mol Life Sci. vol.67

      Pages: 611-628

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] CDP/Cut is an Osteoblastic Co-activator of the Vitamin D receptor (VDR).2010

    • Author(s)
      Ochiai E, Kitagawa K, Takada I, Fujiyama S, Sawatsubashi S, Kim MS, Mezaki Y, Tshushima Y, Takagi K, Azuma Y, Takeyama K, Yamaoka K, Kato S, Kamimura T
    • Journal Title

      J.Bone.Mineral Research vol.25

      Pages: 1157-1166

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Double PHD fingers protein DPF2 recognizes acetylated histones and suppresses the function of ERRα through HDAC.2010

    • Author(s)
      Matsuyama R, Takada I, Yokoyama A, Fujiyma-Nakamura S, Tsuji N, Kitagawa H, Fujiki R, Kim MS, Kouzu-Fujita M, Yano T, Kato S
    • Journal Title

      J.Biol.Chem. vol.285

      Pages: 18166-18176

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of osteoclastic factors in the nuclear envelope of mature, multinucleated osteoclasts.2010

    • Author(s)
      Youn MY, Fujiyama-Nakamura S, Takada I, Imai Y, Kato S
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 74 Pages: 1956-1959

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and identification of estrogen receptor alpha co-regulators in osteoclasts.2010

    • Author(s)
      Takada I, Tsuji N, Youn MY, Fujiyama S, Okada M, Imai Y, Kondo S, Kitakawa H, Yasuda H, Kato S
    • Journal Title

      Ann N Y Acad Sci.

      Volume: 1192 Pages: 201-207

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A histone chaperone, DEK, transcriptionally coactivates a nuclear receptor2010

    • Author(s)
      Sawatsubashi S, Murata T, Lim J, Fujiki R, Ito S, Suzuki E, Tanabe M, Zhao Y, Kimura S, Fujiyama S, et.al.
    • Journal Title

      Genes Dev. 24

      Pages: 159-170

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The metazoan ATAC and SAGA coactivator HAT complexes regulate different sets of inducible target genes2010

    • Author(s)
      Nagy Z, Riss A, Fujiyama S, et.al.
    • Journal Title

      Cell Mol.Life Sci. 67

      Pages: 611-628

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maturation of microRNA is hormonally regulated by a nuclear receptor.2009

    • Author(s)
      Yamagata K, Fujiyama S, Ito S, Ueda T, Murata T, Naitou M, Takeyama K, Minami Y, O'Malley BW, Kato S
    • Journal Title

      Mol.Cell vol.36

      Pages: 340-347

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA demethylation in hormone-induced transcriptional derepression.2009

    • Author(s)
      Kim MS, Kondo T, Takada I, Youn MY, Yamamoto Y, Takahashi S, Matsumoto T, Fujiyama S, Shirode Y, Yamaoka I, Kitagawa H, Takeyama K, Shibuya H, Ohtake F, Kato S
    • Journal Title

      Nature vol.461

      Pages: 1007-1012

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] BTB Protein, dKLHL18/CG3571, serves as an adaptor subunit for a dCul3 ubiquitin ligase complex.2009

    • Author(s)
      Fujiyama-Nakamura S, Ito S, Sawatsubashi S, Yamauchi Y, Suzuki E, Tanabe M, Kimura S, Murata T, Isobe T, Takeyama K, Kato S
    • Journal Title

      Genes Cells vol.14

      Pages: 965-973

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Parvulin (Par14), a peptidyl prolyl cis-trans isomerase, is a novel rRNA-processing factor evolved in the metazoan lineage.2009

    • Author(s)
      Fujiyama-Nakamura S, Yoshikawa H, Homma K, Hayano T, Tsujimura-Takahashi T, Izumikawa K, Ishikawa H, Miyazawa N, Yanagida M, Miura Y, Shinkawa T, Yamauchi Y, Isobe T, Takahashi N
    • Journal Title

      Mol.Cell.Proteomics vol.8

      Pages: 1552-1565

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Maturation of microRNA is hormonally regulated by a nuclear receptor2009

    • Author(s)
      Yamagata K, Fujiyama S, et.al.
    • Journal Title

      Molecular Cell 36

      Pages: 340-347

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA demethylation in hormone-induced transcriptional derepression2009

    • Author(s)
      Kim MS, Kondo T, Takada I, Youn MY, Yamamoto Y, Takahashi S, Matsumoto T, Fujiyama S, et al.
    • Journal Title

      Nature 461

      Pages: 1007-1012

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BTB Protein, dKLHL18/CG3571, serves as an adaptor subunit for a dCul3 ubiquitin ligase complex2009

    • Author(s)
      Fujiyama-Nakamura S, Ito S, et.al.
    • Journal Title

      Genes to Cells 14

      Pages: 965-973

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parvulin(Par14), a peptidyl prolyl cis-trans isomerase, is a novel rRNA-processing factor evolved in the metazoan lineage2009

    • Author(s)
      Fujiyama-Nakamura S, Yoshikawa H, et.al.
    • Journal Title

      Mol.Cell.Proteomics 8

      Pages: 1552-1565

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 核内巨大分子量複合体を介したクロマチン構造調節機構の解明2011

    • Author(s)
      藤山(中村)沙理
    • Organizer
      転写研究会若手シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2011-02-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 核内巨大分子量複合体を介したクロマチン構造調節機構の解明2011

    • Author(s)
      藤山(中村)沙理
    • Organizer
      転写研究会 若手シンポジウム
    • Place of Presentation
      東北大学東京分室 会議室
    • Year and Date
      2011-02-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Identification and characterization of a D12 mega size complex2010

    • Author(s)
      Sally Fujiyama-Nakamura, Saya Ito, Shun Sawatsubashi, Eriko Suzuki, Masahiko Tanabe, Shuhei Kimura, Takashi Ueda, Takuya Murata, Ken-ichi Takeyama, Shigeaki Kato
    • Organizer
      14^<th> International Congress of Endocrinology
    • Place of Presentation
      京都
    • Year and Date
      2010-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Identification and characterization of a D12 mega size complex2010

    • Author(s)
      中村(藤山)沙理, 伊藤紗弥, 沢津橋俊, 鈴木絵里子, 田辺真彦, 木村周平, 上田崇, 村田拓哉, 武山健一, 加藤茂明
    • Organizer
      国際内分泌学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2010-03-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] 遺伝学的アプローチとプロテオミクスを連携した新規活性化クロマチン構造調節因子D12の複合体解析2010

    • Author(s)
      中村沙理, 伊藤紗弥, 沢津橋俊, 鈴木絵里子, 田辺真彦, 木村周平, 上田崇, 村田拓哉, 松川紘之, 林珍仙, 武山健一, 加藤茂明
    • Organizer
      日本農芸化学学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 遺伝学的アプローチとプロテオミクスを連携した新規活性化クロマチン構造調節因子D12の複合体解析2010

    • Author(s)
      中村(藤山)沙理, 伊藤紗弥, 沢津橋俊, 鈴木絵里子, 田辺真彦, 木村周平, 上田崇, 村田拓哉, 林珍仙, 武山健一, 加藤茂明
    • Organizer
      日本農芸化学学会
    • Place of Presentation
      東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Book] 新遺伝子工学ハンドブック(「質量分析計を用いたタンパク質複合体解析」)2010

    • Author(s)
      藤山(中村)沙理, 加藤茂明
    • Publisher
      羊土社
    • Related Report
      2010 Final Research Report
  • [Book] Regulation of MicroRNAs(Repression of pri-miRNA processing through Eralpha)(Editor ; Grosshans H.)2010

    • Author(s)
      Fujiyama-Nakamura S., Yamagata K., Kato S.
    • Publisher
      Landes Bioscience
    • Related Report
      2010 Final Research Report
  • [Book] 現代栄養学を理解するための分子生物学入門(「プロテオミクス」「遺伝子ノックダウン」)2010

    • Author(s)
      藤山(中村)沙理著, 加藤茂明編
    • Publisher
      光生館
    • Related Report
      2010 Final Research Report
  • [Book] 新遺伝子工学ハンドブック2010

    • Author(s)
      藤山(中村)沙理, 加藤茂明
    • Total Pages
      5
    • Publisher
      羊土社
    • Related Report
      2010 Annual Research Report
  • [Book] Regulation of Micro RNAs(Repression of pri-miRNA processing through ERalpha部分)2010

    • Author(s)
      Fujiyama-Nakamura S., Yamagata K., Kato S
    • Total Pages
      13
    • Publisher
      Landes Bioscience
    • Related Report
      2009 Annual Research Report
  • [Book] がん分子標的治療研究実践マニュアル(LC-MS/MS)(日本がん分子標的治療学会編)2009

    • Author(s)
      藤山沙理, 加藤茂明著
    • Publisher
      金芳堂
    • Related Report
      2010 Final Research Report
  • [Book] がん分子標的治療研究 実践マニュアル(LC-MS/MS部分)2009

    • Author(s)
      藤山沙理, 加藤茂明
    • Total Pages
      6
    • Publisher
      金芳堂
    • Related Report
      2009 Annual Research Report
  • [Book] 現代栄養学を理解するための分子生物学入門(「プロテオミクス」「遺伝子ノックダウン」部分)2009

    • Author(s)
      藤山(中村)沙理
    • Total Pages
      7
    • Publisher
      光生館
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.iam.u-tokyo.ac.jp/bnsikato/index.html

    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-03-31   Modified: 2016-04-21  

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