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Function of selenium-delivering proteins and regulation of selenium trafficking

Research Project

Project/Area Number 21780094
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied biochemistry
Research InstitutionRitsumeikan University

Principal Investigator

MIHARA Hisaaki  Ritsumeikan University, 生命科学部, 准教授 (30324693)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsセレン / 微量元素 / タンパク質 / セレノシステイン / セレンタンパク質 / 翻訳
Research Abstract

In this study, proteins and enzymes involved in selenium metabolism and selenoprotein biosynthesis are investigated by crystal structural analysis of proteins in the form of a complex with a reaction intermediate, kinetic analysis, and ESI-MS analysis. Structure-function relationships of selenium-specific chemical reactions by the proteins and enzymes were revealed, establishing a molecular basis for the function of selenium as a trace element.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (51 results)

All 2011 2010 2009

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (30 results)

  • [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures.2011

    • Author(s)
      Kawamoto J, Sato T, Nakasone K, Kato C, Mihara H, Esaki N, Kurihara T.
    • Journal Title

      Environ Microbiol In press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil.2011

    • Author(s)
      Hidese R, Mihara H, Kurihara T, Esaki N.
    • Journal Title

      J Bacteriol 193

      Pages: 989-993

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Escherichia coli Dihydropyrimidine Dehydrogenase Is a Novel NAD-Dependent Heterotetramer Essential for the Production of 5,6-Dihydrouracil2011

    • Author(s)
      Hidese R., et al.
    • Journal Title

      JOURNAL OF BACTERIOLOGY

      Volume: 193 Pages: 989-993

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IscS Functions as a Primary Sulfur-donating Enzyme by Interacting Specifically with MoeB and MoaD in the Biosynthesis of Molybdopterin in Escherichia coli.2010

    • Author(s)
      Zhang WJ, Urban A, Mihara H, Leimkuhler S, Kurihara T, Esaki N.
    • Journal Title

      J.Biol.Chem. 285

      Pages: 2302-2308

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of phosphatidyl-D-serine in rat.2010

    • Author(s)
      Omori T, Mihara H, Kurihara T, Esaki N.
    • Journal Title

      Biosci.Biotechnol. Biochem. 74

      Pages: 1953-1955

    • NAID

      10027559956

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reaction mechanism and molecular basis for selenium/sulfur discrimination of selenocysteine lyase.2010

    • Author(s)
      Omi R, Kurokawa S, Mihara H, Hayashi H, Goto M, Miyahara I, Kurihara T, Hirotsu K, Esaki N.
    • Journal Title

      J.Biol.Chem. 285

      Pages: 12133-12139

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Distribution of Phosphatidyl-D-serine in the Rat2010

    • Author(s)
      Omori T., et al.
    • Journal Title

      BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

      Volume: 74 Pages: 1953-1955

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reaction Mechanism and Molecular Basis for Selenium/Sulfur Discrimination of Selenocysteine Lyase2010

    • Author(s)
      Omi R., et al.
    • Journal Title

      JOURNAL OF BIOLOGICAL CHEMISTRY.

      Volume: 285 Pages: 12133-12139

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reaction mechanism and molecular basis for selenium/sulfur discrimination of selenocysteine lyase2010

    • Author(s)
      Omi, R.
    • Journal Title

      J.Biol.Chem. 285

      Pages: 12133-12139

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli2010

    • Author(s)
      Zhang, W.
    • Journal Title

      J.Biol.Chem. 285

      Pages: 2302-2308

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic Synthesis of Unnatural Amino Acids.2009

    • Author(s)
      Yasuda MH, Ueda M, Okano K, Mihara H, Esaki N.
    • Journal Title

      Asymmetric Synthesis and Application of α-Amino Acids 1009

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serine racemase with catalytically active lysinoalanyl residue.2009

    • Author(s)
      Yamauchi T, Goto M, Wu HY, Uo T, Yoshimura T, Mihara H, Kurihara T, Miyahara I, Hirotsu K, Esaki N.
    • Journal Title

      J.Biochem. 145

      Pages: 421-424

    • NAID

      10026301897

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of proteins interacting with selenocysteine lyase.2009

    • Author(s)
      Tobe R, Mihara H, Kurihara T, Esaki N.
    • Journal Title

      Biosci.Biotechnol. Biochem. 73

      Pages: 1230-1232

    • NAID

      10027541676

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Occurrence of phosphatidyl-D-serine in the rat cerebrum.2009

    • Author(s)
      Omori T, Mihara H, Kurihara T, Esaki N.
    • Journal Title

      Biochem.Biophys.Res.Commun. 382

      Pages: 415-418

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Discrimination between selenium and sulfur by enzymes [Japanese].2009

    • Author(s)
      Mihara H.
    • Journal Title

      Biomed.Res.Trace Elem. 20

      Pages: 218-225

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] X-Ray Crystallographic and Mutational Studies of Fluoroacetate Dehalogenase from Burkholderia sp Strain FA1.2009

    • Author(s)
      Jitsumori K, Omi R, Kurihara T, Kurata A, Mihara H, Miyahara I, Hirotsu K, Esaki N.
    • Journal Title

      J.Bacteriol. 191

      Pages: 2630-2637

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selenocysteine is selectively taken up byred blood cells.2009

    • Author(s)
      Imai T, Mihara H, Kurihara T, Esaki N.
    • Journal Title

      Biosci.Biotechnol.Biochem. 73

      Pages: 2746-2748

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible Role of Red Blood Cells in Selenocysteine Metabolism Trace2009

    • Author(s)
      Imai T, Mihara H, Kurihara T, Esaki N.
    • Journal Title

      Nutri.Res. 26

      Pages: 22-25

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 大腸菌由来新規NADH 依存性ジヒドロピリミジンデヒドロゲナーゼの機能解析2009

    • Author(s)
      Hidese R, Mihara H, Esaki N.
    • Journal Title

      ビタミン 83

      Pages: 528-532

    • NAID

      110007387826

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal Structure of a Homolog of Mammalian Serine Racemase from Schizosaccharomyces pombe.2009

    • Author(s)
      Goto M, Yamauchi T, Kamiya N, Miyahara I, Yoshimura T, Mihara H, Kurihara T, Hirotsu K, Esaki N.
    • Journal Title

      J.Biol.Chem. 284

      Pages: 25944-25952

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selenocysteine is selectively taken up by red blood cells2009

    • Author(s)
      Imai, T.
    • Journal Title

      Biosci.Biotechnol.Biochem. 73

      Pages: 2746-2748

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 金属汚染浄化とレアメタル資源回収システムの開発を目指した新規金属代謝微生物の研究2011

    • Author(s)
      三原久明
    • Organizer
      長瀬科学技術振興財団平成22年度研究成果発表会
    • Place of Presentation
      大阪
    • Year and Date
      2011-04-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Bacillus 属細菌のセレン酸還元メカニズムの解析2011

    • Author(s)
      斎藤茂樹, 谷泰史, Prakash T, 三原久明
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学(京都府)
    • Year and Date
      2011-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 鉄硫黄クラスター生合成機構の新規な解析法2011

    • Author(s)
      秀瀬涼太, 三原久明, 栗原達夫, 江崎信芳
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学(京都府)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 微生物によるレアメタルの回収2011

    • Author(s)
      三原久明
    • Organizer
      立命館グローバル・イノベーション研究機構(R-GIRO)材料研究拠点シンポジウム
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス(滋賀県)
    • Year and Date
      2011-03-03
    • Related Report
      2010 Final Research Report
  • [Presentation] 亜セレン酸還元経路の探索2010

    • Author(s)
      今井岳志, 三原久明, 栗原達夫, 江崎信芳
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] Escherichia coli由来ジヒドロピリミジンデヒドロゲナーゼの反応機構解析2010

    • Author(s)
      吉田吉孝, 他
    • Organizer
      BMB2010
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 鉄硫黄クラスターおよびセレンタンパク質の生合成を司る硫黄・セレン脱離酵素群の発見と分子機能解析2010

    • Author(s)
      三原久明
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 鉄硫黄クラスターおよびセレンタンパク質の生合成を司る硫黄・セレン脱離酵素群の発見と分子機能解析2010

    • Author(s)
      三原久明
    • Organizer
      BMB2010
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pseudomonas putida KT2440 由来ジヒドロピリミジンデヒドロゲナーゼの酵素学的性質と生理的役割.2010

    • Author(s)
      秀瀬涼太, 三原久明, 栗原達夫, 江崎信芳
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Final Research Report
  • [Presentation] 好熱性嫌気性細菌由来2,4-ジアミノペンタン酸デヒドロゲナーゼの機能解析2010

    • Author(s)
      福山貞伸, 栗原達夫, 川端潤, 三原久明, 上田誠, 江崎信芳
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2010-12-07
    • Related Report
      2010 Final Research Report
  • [Presentation] レアメタル資源回収システムの開発を目指した新奇金属代謝微生物の研究2010

    • Author(s)
      谷泰史, 道本麻衣, 斎藤茂樹, 三原久明
    • Organizer
      第2回メタロミクス研究フォーラム
    • Place of Presentation
      京都薬科大学(京都府)
    • Year and Date
      2010-11-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Geobacter sulfurreducens 由来新奇マルチヘムセレノプロテイン2010

    • Author(s)
      三原久明, 張万皎, 栗原達夫, 江崎信芳
    • Organizer
      2010年度酵素・補酵素研究会
    • Place of Presentation
      西日本総合展示場(福岡県)
    • Year and Date
      2010-09-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Geobacter sulfurreducens 由来新奇マルチヘムセレノプロテイン2010

    • Author(s)
      三原久明, 張万皎, 栗原達夫, 江崎信芳
    • Organizer
      特殊環境微生物セミナー
    • Place of Presentation
      京都大学宇治キャンパス(京都府)
    • Year and Date
      2010-07-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Physiological function and regulation of mammalian selenocysteine lyase.2010

    • Author(s)
      Kurokawa S, Winfrey V, Takehashi M, Tanaka H, Mihara H, Kurihara T, Tanaka S, Olson G, Hill K, Burk R.
    • Organizer
      9th International Symposium on Selenium in Biology and Medicine (Selenium 2010).
    • Place of Presentation
      Kyoto University (Kyoto, Japan)
    • Year and Date
      2010-06-02
    • Related Report
      2010 Final Research Report
  • [Presentation] The uptake of selenium compounds by RBCs and selenite reduction by glutathione reductase.2010

    • Author(s)
      Imai T, Mihara H, Kurihara T, Esaki N.
    • Organizer
      9th International Symposium on Selenium in Biology and Medicine (Selenium 2010).
    • Place of Presentation
      Kyoto University(Kyoto, Japan)
    • Year and Date
      2010-06-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Discovery of a novel heme-containing selenoprotein from Geobacter sulfurreducens.2010

    • Author(s)
      Zhang W, Mihara H, Kurihara T, Esaki N.
    • Organizer
      9th International Symposium on Selenium in Biology and Medicine (Selenium 2010).
    • Place of Presentation
      Kyoto University (Kyoto, Japan)
    • Year and Date
      2010-06-01
    • Related Report
      2010 Final Research Report
  • [Presentation] Why Sps1 and Sps2 differ in their catalysis?: Identification of an internal sequence region catalytically essential for the human lung selenophosphate synthetase 2.2010

    • Author(s)
      Okugochi T, Tamura T, Takahata M, Mihara H, Kurokawa S, Abe K, Esaki N, Inagaki K.
    • Organizer
      9th International Symposium on Selenium in Biology and Medicine (Selenium 2010).
    • Place of Presentation
      Kyoto University (Kyoto, Japan)
    • Year and Date
      2010-06-01
    • Related Report
      2010 Final Research Report
  • [Presentation] 乳酸菌由来N-メチル-L-アミノ酸オキシダーゼの大腸菌での発現と諸性質2010

    • Author(s)
      谷川昌寛, 矢野成和, 三原久明, 立木隆, 若山守
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学駒場Iキャンパス(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 必須微量元素セレンのタンパク質取込に関連するタンパク質の機能2010

    • Author(s)
      三原久明, 江崎信芳
    • Organizer
      ビタミンB研究委員会シンポジウム
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府)
    • Year and Date
      2010-02-05
    • Related Report
      2010 Final Research Report
  • [Presentation] ラットにおけるホスファチジル-D-セリンの同定2009

    • Author(s)
      大森勇門, 三原久明, 栗原達夫, 江崎信芳
    • Organizer
      2009年度合同沖縄大会(日本農芸化学会関西・中四国・西日本支部、日本栄養・食糧学会九州・沖縄支部、日本食品科学工学西日本支部)
    • Place of Presentation
      琉球大学千原キャンパス(沖縄県)
    • Year and Date
      2009-10-31
    • Related Report
      2010 Final Research Report
  • [Presentation] ジヒドロピリミジンデヒドロゲナーゼ活性に影響を与える遺伝子の探索2009

    • Author(s)
      秀瀬涼太, 三原久明, 栗原達夫, 江崎信芳
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-10-23
    • Related Report
      2010 Final Research Report
  • [Presentation] セレン輸送体はモノチオール型ではなくジチオール型蛋白質である2009

    • Author(s)
      田村隆, 佐藤久美, 今井岳志, 三原久明, 江崎信芳, 稲垣賢二.
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-10-23
    • Related Report
      2010 Final Research Report
  • [Presentation] セレノリン酸合成酵素Sps1 はSps2 抑制型アンチザイム機能を持つ2009

    • Author(s)
      奥河内貴大, 田村隆, 高畑宗明, 三原久明, 江崎信芳, 稲垣賢二
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-10-23
    • Related Report
      2010 Final Research Report
  • [Presentation] モリブドプテリン合成酵素と特異的に相互作用するシステインデスルフラーゼの同定2009

    • Author(s)
      Zhang W, 三原久明, 栗原達夫, 江崎信芳
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-10-23
    • Related Report
      2010 Final Research Report
  • [Presentation] セレノシステイン残基の生成機構:セレノシステイルtRNAの生成を担う酵素群の解析2009

    • Author(s)
      三原久明, 栗原達夫, 江崎信芳
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-10-22
    • Related Report
      2010 Final Research Report
  • [Presentation] セレノシステイン残基の生成機構:セレノシステイル-tRNAの生成を担う酵素群の解析2009

    • Author(s)
      三原久明
    • Organizer
      第82回日本生化学会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report
  • [Presentation] 含硫黄・含セレンバイオファクターの生成に関わる酵素の機能・構造解析2009

    • Author(s)
      三原久明
    • Organizer
      BioJapan2009
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2009-10-07
    • Related Report
      2010 Final Research Report
  • [Presentation] セレンタンパク質生合成におけるメタン生成アーキア由来セリルtRNA 合成酵素の新機能2009

    • Author(s)
      三原久明, 戸部隆太, 栗原達夫, 江崎信芳
    • Organizer
      2009年度酵素・補酵素研究会
    • Place of Presentation
      アジール奈良(奈良県)
    • Year and Date
      2009-07-10
    • Related Report
      2010 Final Research Report
  • [Presentation] セレンタンパク質生合成におけるセレン特異的運搬2009

    • Author(s)
      三原久明, 戸部隆太, 栗原達夫, 江崎信芳
    • Organizer
      第20回日本微量元素学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2009-07-03
    • Related Report
      2010 Final Research Report
  • [Presentation] 血球細胞へのセレノシステインの取り込み2009

    • Author(s)
      三原久明, 今井岳志, 栗原達夫, 江崎信芳
    • Organizer
      日本微量栄養素学会第26回学術集会
    • Place of Presentation
      京都リサーチパーク(京都府)
    • Year and Date
      2009-06-05
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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