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A proteomic approach to cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis

Research Project

Project/Area Number 21780166
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Wood science
Research InstitutionOkinawa Institute of Science and Technology (2010)
Kyoto University (2009)

Principal Investigator

NAKASHIMA Keisuke  Okinawa Institute of Science and Technology, マリンゲノミックスユニット, 研究員 (10422924)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsセルロース / ホヤ / セルロース合成酵素 / 膜タンパク複合体 / ターミナル・コンプレックス / セルロース合成複合体 / カタユウレイボヤ / 動物 / プロテオミクス
Research Abstract

We report a western blotting system to assess cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis. Rabbit polyclonal antibodies were raised against several synthetic peptides corresponding to the parts of Ci-CesA, which is the cellulose synthase of C. intestinalis. After purifications with affinity columns using the peptides and an adsorption with Ciona acetone powder, either non-specific reactivity or cross-reactivity was reduced so that the single band was obtained with the estimated molecular mass of Ci-CesA, only in microsome preparations. For sample preparations, French-press worked fine for Ciona embryos.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (15 results)

All 2011 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (4 results) Remarks (8 results)

  • [Journal Article] The crystalline phase of cellulose changes under developmental control in a marine chordate2011

    • Author(s)
      Keisuke Nakashima, Atsuo Nishino, Yoshiki Horikawa, Euichi Hirose, Junji Sugiyama, Nori Satoh
    • Journal Title

      Cellular and Molecular Life Sciences Vol.68(9)

      Pages: 1623-1631

    • URL

      http://www.springerlink.com/content/mgt2v3640830xj32/

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The crystalline phase of cellulose changes under developmental control in a marine chordate2011

    • Author(s)
      Keisuke Nakashima
    • Journal Title

      Cellular and Molecular Life Sciences

      Volume: 68 Pages: 1623-1631

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Forming a tough shell via an intracellular matrix and cellular junctions in the tail epidermis of Oikopleura dioica (Chordata : Tunicata : Appendicularia)

    • Author(s)
      Keisuke Nakashima, Atsuo Nishino, Euichi Hirose
    • Journal Title

      Naturwissenschaften in press

    • URL

      http://www.springerlink.com/content/g453445h7h3321r2/

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] 動物がつくるセルロース2011

    • Author(s)
      中島啓介
    • Organizer
      OISTシンポジウム「動物の不思議な能力~ゲノムからの挑戦」(口頭発表)
    • Place of Presentation
      沖縄産業支援センター(那覇市)
    • Year and Date
      2011-01-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 動物がつくるセルロース2011

    • Author(s)
      中島啓介
    • Organizer
      OISTシンポジウム「動物の不思議な能力~ゲノムからの挑戦」
    • Place of Presentation
      沖縄産業支援センター
    • Year and Date
      2011-01-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cellulosic structures of oikopleurid appendicularians2009

    • Author(s)
      Keisuke Nakashima
    • Organizer
      The 5th International Tunicate Meeting
    • Place of Presentation
      那覇市・沖縄産業支援センター
    • Year and Date
      2009-06-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cellulosic structures of Oikopleurid appendicularians2009

    • Author(s)
      Keisuke Nakashima
    • Organizer
      The 5th International Tunicate Meeting(口頭発表)
    • Place of Presentation
      那覇
    • Year and Date
      2009-06-23
    • Related Report
      2010 Final Research Report
  • [Remarks] 所属部局サイト:独立行政法人沖縄科学技術研究基盤整備機構マリンゲノミックスユニット

    • URL

      http://www.irp.oist.jp/satoh/

    • Related Report
      2010 Final Research Report
  • [Remarks] 雑誌論文(1)に関するプレスリリースのサイト:英語版

    • URL

      http://www.oist.jp/en/press-room/pressreleases/40-2010/696-dr-keisuke-nakashima-and-dr-satoh-elucidates-mechanism-of-cellulose-synthesis-in-appendicularian.html

    • Related Report
      2010 Final Research Report
  • [Remarks] 日本語版

    • URL

      http://www.oist.jp/ja/press-room/pressreleases/40-2010/696-dr-keisuke-nakashima-and-dr-satoh-elucidates-mechanism-of-cellulose-synthesis-in-appendicularian.html

    • Related Report
      2010 Final Research Report
  • [Remarks] 学会発表(1)に関するサイト:The 5th International Tunicate Meeting

    • URL

      http://www.irp.oist.jp/tunicatemeeting/dp.php

    • Related Report
      2010 Final Research Report
  • [Remarks] 学会発表(2)に関するサイト

    • URL

      http://www.oist.jp/ja/events/event-reports/745-oist-symposium-on-extraordinary-abilities-of-animals.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.oist.jp/en/press-room/press-releases/40-2010/696-dr-keisuke-nakashima-and-dr-satoh-elucidates-mechanism-of-cellulose-synthesis-in-appendicularian.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.oist.jp/ja/press-room/press-releases/40-2010/696-dr-keisuke-nakashima-and-dr-satoh-elucidates-mechanism-of-cellulose-synthesis-in-appendicularian.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.oist.jp/ja/events/event-reports/745-oist-symposium-on-extraordinary-abilities-of-animals.html

    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-10-28  

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