A proteomic approach to cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis
| Project/Area Number |
21780166
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Wood science
|
|---|
| Research Institution | Okinawa Institute of Science and Technology (2010)
Kyoto University (2009) |
|---|
Principal Investigator |
NAKASHIMA Keisuke Okinawa Institute of Science and Technology, マリンゲノミックスユニット, 研究員 (10422924)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | セルロース / ホヤ / セルロース合成酵素 / 膜タンパク複合体 / ターミナル・コンプレックス / セルロース合成複合体 / カタユウレイボヤ / 動物 / プロテオミクス |
|---|
| Research Abstract |
We report a western blotting system to assess cellulose-synthesizing membrane protein complexes in the ascidian Ciona intestinalis. Rabbit polyclonal antibodies were raised against several synthetic peptides corresponding to the parts of Ci-CesA, which is the cellulose synthase of C. intestinalis. After purifications with affinity columns using the peptides and an adsorption with Ciona acetone powder, either non-specific reactivity or cross-reactivity was reduced so that the single band was obtained with the estimated molecular mass of Ci-CesA, only in microsome preparations. For sample preparations, French-press worked fine for Ciona embryos.
|
Report
(3 results)
Research Products
(15 results)
