Development of utilization method for unused biomass using marine lactic acid bacteria

• Project/Area Number: 21780199
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Fisheries chemistry
• Research Institution: Nihon University
• Principal Investigator: ITOI Shiro Nihon University, 生物資源科学部, 講師 (30385992)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 生物工学 / 海洋細菌 / 海洋性乳酸菌 / キチンオリゴ糖 / Lactococcus lactis subsp. lactis / バイオプラスチック / 乳酸 / 未利用バイオマス / Lactococcus lactis subsp.lactis

Research Abstract

To utilize effectively chitin and the associated materials which is one of unused biomass, availability of chitin-associated sugars in lactic acid bacteria, Lactococcus lactis, derived from marine fish was compared with those from other sources, such as cheese starter and freshwater fish. As the results, fermentation of chitin oligosaccharides in the strains derived from fish were observed in the media containing GlcNAc-(GlcNAc)_6, whereas those from cheese starter were observed in GlcNAc-(GlcNAc)_3. In addition, ratio of optical isomer in products for L-lactic acid in marine fish derived strain culture was higher than those in other sources. Furthermore, the amount of L-lactic acid in marine strain culture was 3.5-4.4 folds higher than those in other sources.

Research Products

• [Journal Article] Diversity of siderophore-producing bacteria isolated from the intestinal tracts of fish along the Japanese coast. (2010)
  • Author(s): Sugita, H., Mizuki, H., Itoi, S.
  • Journal Title: Aquaculture Research 印刷中
  • Peer Reviewed
• [Journal Article] Culturable bacterial flora in the intestinal tract of Japanese pufferfish Takifugu rubripes. (2010)
  • Author(s): Sugita, H., Sugiyama, K., Itoi, S.
  • Journal Title: Aquaculture Science 58 Pages: 437-438
  • NAID: 130004888423
  • Peer Reviewed
• [Journal Article] Culturable bacterial flora in the intestinal tract of Japanese pufferfish Takifugu rubripes (2010)
  • Author(s): Sugita, H., Sugiyama, K., Itoi, S.
  • Journal Title: Aquaculture Science Volume: 58 Pages: 437-438
  • NAID: 130004888423
  • Peer Reviewed
• [Journal Article] The effect of Lactococcus lactis on the abundance of aeromonads in the rearing water of the goldfish, Carassius auratus (Linnaeus). (2009)
  • Author(s): Sugita, H., Fujie, T., Sagesaka, T., Itoi, S.
  • Journal Title: Aquaculture Research 41 Pages: 153-156
  • Peer Reviewed
• [Journal Article] Phenotypic variation in Lactococcus lactis subsp. lactis isolates derived from intestinal tracts of marine and freshwater fishes. (2009)
  • Author(s): Itoi, S., Yuasa, K., Washio, S., Abe, T., Ikuno, E., Sugita, H.
  • Journal Title: Journal of Applied Microbiology 107 Pages: 867-874
  • Peer Reviewed
• [Journal Article] Phenotypic variation in Lactococcus lactis subsp.lactis isolates derived from intestinal tracts of marine and freshwater fishes (2009)
  • Author(s): Shiro Itoi, Kohei Yuasa, Sayaka Washio, Takeshi Abe, Erika Ikuno, Hamo Sugita
  • Journal Title: Journal of Applied Microbiology 107 Pages: 867-874
  • Peer Reviewed
• [Journal Article] The effect of Lactococcus lactis on the abundance of aeromonads in the rearing water of the goidfish, Carassius auratus (Linnaeus) (2009)
  • Author(s): Haruo Sugita, Takashi Fujie, Tomoko Sagesaka, Shiro Itoi
  • Journal Title: Aquaculture Research 41 Pages: 153-156
  • Peer Reviewed
• [Journal Article] Diversity of siderophore-producing bacteria isolated from the intestinal tracts of fish along the Japanese coast
  • Author(s): Sugita, H., Mizuki, H., Itoi, S.
  • Journal Title: Aquaculture Research Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Comparison of the ability to utilize chitin oligosaccharides in Lactococcus lactis strains isolated from various sources. (2011)
  • Author(s): Takanashi, S., Oie, R., Itoi, S., Sugita, H.
  • Organizer: 9th Asian Fisheries and Aquaculture Forum
  • Place of Presentation: Shanghai, China.
• [Presentation] Characterization of a marine lactic acid bacterium Lactococcus lactis subsp. lactis isolated from the pufferfish. (2010)
  • Author(s): Itoi, S., Sugita, H.
  • Organizer: 9th International Marine Biotechnology Conference (IMBC2010)
  • Place of Presentation: Qingdao, China.
  • Year and Date: 2010-10-10
• [Presentation] Characterization of a marine lactic acid bacterium Lactococcus lactis subsp.lactisisolated from the pufferfish (2010)
  • Author(s): Itoi, S., Sugita, H.
  • Organizer: 9th International Marine Biotechnology Conference (IMBC2010)
  • Place of Presentation: 中国 チンタオ
  • Year and Date: 2010-10-10