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Localization of inositol 1,4,5 trisphosphate receptor type1 (IP_3R1), the channel responsible for Ca^<2+> release and oscillations during fertilization in mammals

Research Project

Project/Area Number 21780253
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied animal science
Research InstitutionAzabu University

Principal Investigator

ITO Junya  Azabu University, 獣医学部, 准教授 (30454143)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords卵 / 受精 / イノシトール3リン酸受容体 / 哺乳類 / シグナル伝達 / キナーゼ / IP_3受容体
Research Abstract

In mammals, a sperm-induced intracellular Ca^<2+> signal ([Ca^<2+>]i) mediates both exit of meiosis and oocyte activation during fertilization. Recently, it was demonstrated in mouse oocytes that the phosphorylation levels of inositol 1,4,5 trisphosphate receptor type1 (IP_3R1), the channel responsible for Ca^<2+> release and oscillations during fertilization, changed during maturation and fertilization (Ito et al., 2008, Dev. Biol). However, kinetics of IP3R1 is not well understood in other species, especially domestic species which have low developmental ability of oocytes when intracytoplasmic sperm injection (ICSI) and somatic cell nuclear transfer (SCNT) are applied. Therefore, we examined the expression, phosphorylation and localization of IP_3R1 during in vitro maturation of pig oocytes. Here, our present study shows that expression of IP_3R1 protein did not change during maturation, although the phosphorylation status of the receptor was dramatically upregulated beyond germinal vesicle break down (GVBD). At the GV stage, IP_3R1 was observed throughout the cytoplasm. Following oocyte maturation, concentration of IP_3R1 protein at the cortex of the oocyte was confirmed. In matured oocytes, large clusters of IP_3R1 were formed in the cortex of the oocyte except in a ring-shaped band of cortex adjacent to the spindle. Taken together, our results show that IP_3R1 undergoes dynamic phosphorylation and localization during maturation and this might underlie the generation of oscillations at fertilization. Our findings will contribute to improve the efficiency of ICSI and SCNT in domestic species.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (18 results)

All 2011 2010 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (4 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Ethylene glycol-supplemented calcium-free media improve zona penetration of vitrified rat oocytes by sperm cells.2010

    • Author(s)
      Fujiwara K, Sano D, Seita Y, Inomata T, Ito J, et al.
    • Journal Title

      Journal of Reproduction and Development 56

      Pages: 169-175

    • NAID

      10026324070

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of inositol 1,4,5-triphosphate receptor 1 during in vitro maturation of porcine oocytes.2010

    • Author(s)
      Ito J, et al.
    • Journal Title

      Animal Science Journal 81

      Pages: 34-41

    • NAID

      10027888417

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of phosphatidylinositol 3-kinase in the maintenance of metaphase II attest in porcine oocytes matured in vitro.2010

    • Author(s)
      Ito J, et al.
    • Journal Title

      Animal Science Journal 81

      Pages: 42-47

    • NAID

      10027888464

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryotop facilitates high developmental ability of re-vitrified mouse embryos2010

    • Author(s)
      Ito, et al.
    • Journal Title

      Journal of Reproduction Engineering

      Volume: 13 Pages: 21-26

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear replacement of in vitro matured porcine oocytes by a serial centrifugation and fusion method2010

    • Author(s)
      Maedomari, et al.
    • Journal Title

      Reproduction in Domestic Animals

      Volume: 45 Pages: 659-665

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of the MAPK pathway has an essential role in the acrosome reaction in miniature pig sperm2010

    • Author(s)
      Kawano, et al.
    • Journal Title

      Reproduction in Domestic Animals

      Volume: 45 Pages: 263-268

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of inositol 1,4,5-triphosphate receptor 1 during in vitro maturation of porcine oocytes.2010

    • Author(s)
      Ito J., et al.
    • Journal Title

      Animal Science Journal 81

      Pages: 34-41

    • NAID

      10027888417

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible involvement of phosphatidylinositol 3-kinase in the maintenance of meta phase II attest in porcine oocytes matured in vitro.2010

    • Author(s)
      Ito J., et al.
    • Journal Title

      Animal Science Journal 81

      Pages: 42-47

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pre-Treatment of sperm reduces success of intracytoplasmic sperm injection in the pig.

    • Author(s)
      Nakai M, Ito J, et al.
    • Journal Title

      Reproduction accepted

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Factors affecting fertilization and embryonic development during intracytoplasmic sperm injection in pigs.

    • Author(s)
      Nakai M, Kashiwazaki N, Ito J, et al.
    • Journal Title

      Journal of Reproduction and Development accepted

    • NAID

      10028275004

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Full-term development of rats from oocytes fertilized in vitro using cryopreserved ejaculated sperm

    • Author(s)
      Seita, et al.
    • Journal Title

      Cryobiology

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Factors affecting fertilization and embryonic development during intracytoplasmic sperm injection in pigs

    • Author(s)
      Nakai, et al.
    • Journal Title

      Journal of Reproduction and Development

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 超低温保存時の要因が哺乳類未受精卵・初期胚に及ぼす影響2010

    • Author(s)
      伊藤潤哉
    • Organizer
      日本生殖工学会
    • Place of Presentation
      リバティータワー(東京)
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] 実験動物における卵の超低温保存2010

    • Author(s)
      伊藤潤哉, 他
    • Organizer
      第28回日本受精着床学会総会・学術講演会
    • Related Report
      2010 Final Research Report
  • [Presentation] 実験動物における卵の超低温保存2010

    • Author(s)
      伊藤潤哉, 他
    • Organizer
      第27回日本受精着床学会総会・学術講演会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2010 Annual Research Report
  • [Presentation] Factors associated with calcium oscillations during fertilization in mammal2010

    • Author(s)
      Ito, et al.
    • Organizer
      Czech-Japan Joint Symposium for Animal Reproduction
    • Place of Presentation
      チェコ
    • Related Report
      2010 Annual Research Report
  • [Book] 動物応用科学の展開2011

    • Author(s)
      伊藤潤哉, 他
    • Publisher
      養賢堂
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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