The role of vector saliva in transmission of vector-borne diseases

• Project/Area Number: 21780276
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: Yamaguchi University
• Principal Investigator: KATO Hirotomo Yamaguchi University, 農学部, 准教授 (00346579)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: サシチョウバエ / 唾液 / リーシュマニア / 生理活性物質 / 免疫 / ワクチン / 液性免疫

Research Abstract

In order to disclose the mechanism of enhancement of Leishmania infection by sand fly saliva, DNA vaccines encoding dominant salivary proteins from Phlebotomus duboscqi were prepared and immune responses induced by these antigens were assessed. As the result, three salivary proteins were found to effectively induce humoral responses in mice. Since humoral immune responses were shown to exacerbate Leishmania infection, these salivary proteins were suggested to associate with the enhancement of the infection via host immunity.

Research Products

• [Journal Article] Natural infections of man-biting sand flies by Leishmania and Trypanosoma species in the northern Peruvian Andes. (2011)
  • Author(s): Kato H
  • Journal Title: Vector Borne Zoonotic Dis Volume: (in press)
  • Peer Reviewed
• [Journal Article] Use of FTA cards for direct sampling of patients' lesions in the ecological study of cutaneous leishmaniasis. (2010)
  • Author(s): Kato H, Caceres AG, Mimori T, Ishimaru Y, Sayed ASM, Fujita M, Iwata H, Uezato H, Velez LN, Gomez EAL, Hashiguchi Y.
  • Journal Title: J Clin Microbiol 48 Pages: 3661-3665
  • Peer Reviewed
• [Journal Article] A repertoire of the dominant transcripts from the salivary glands of the blood-sucking bug, Triatoma dimidiata, a vector of Chagas disease. (2010)
  • Author(s): Kato H, Jochim RC, Gomez EA, Sakoda R, Iwata H, Valenzuela JG, Hashiguchi Y.
  • Journal Title: Infect Genet Evol. 10 Pages: 184-191
  • Peer Reviewed
• [Journal Article] Natural infection of the sand fly Phlebotomus kazeruni by Trypanosoma species in Pakistan. (2010)
  • Author(s): Kato H, Uezato H, Sato H, Bhutto AM, Soomro FR, Baloch JH, Iwata H, Hashiguchi Y.
  • Journal Title: Parasit Vectors. 3 Pages: 10-10
  • Peer Reviewed
• [Journal Article] Molecular epidemiology for vector research on leishmaniasis. (2010)
  • Author(s): Kato H, Gomez EA, Caceres AG, Uezato H, Mimori T, Hashiguchi Y.
  • Journal Title: Int J Environ Res Public Health. 7 Pages: 814-826
  • Peer Reviewed
• [Journal Article] Use of FTA cards for direct sampling of patients' lesions in the ecological study of cutaneous leishmaniasis. (2010)
  • Author(s): Kato H
  • Journal Title: J Clin Microbiol Volume: 48 Pages: 3661-3665
  • Peer Reviewed
• [Journal Article] A repertoire of the dominant transcripts from the salivary glands of the blood-sucking bug, Triatoma dimidiata, a vector of Chagas disease (2010)
  • Author(s): Kato H
  • Journal Title: Infect Genet Evol. 10 Pages: 184-191
  • Peer Reviewed
• [Journal Article] Natural infection of the sand fly Phlebotomus kazeruni by Trypanosoma species in Pakistan (2010)
  • Author(s): Kato H
  • Journal Title: Parasites & Vectors 3 Pages: 10-10
  • Peer Reviewed
• [Journal Article] Molecular Epidemiology for Vector Research on Leishmaniasis (2010)
  • Author(s): Kato H
  • Journal Title: Int J Environ Res Public Health 7 Pages: 814-826
  • Peer Reviewed
• [Journal Article] Genetic diversity of ribosomal RNA internal transcribed spacer sequences in Lutzomyia species from areas endemic for New World cutaneous leishmaniasis. (2009)
  • Author(s): Kuwahara K, Kato H, Gomez EA, Uezato H, Mimori T, Yamamoto YI, Calvopina M, Caceres AG, Iwata H, Hashiguchi Y.
  • Journal Title: Acta Trop. 112 Pages: 131-136
  • Peer Reviewed
• [Journal Article] Functional characterization of a salivary apyrase from the sand fly, Phlebotomus duboscqi, a vector of Leishmania major. (2009)
  • Author(s): Hamasaki R, Kato H, Terayama Y, Iwata H, Valenzuela JG.
  • Journal Title: J Insect Physiol. 55 Pages: 1044-1049
  • Peer Reviewed
• [Journal Article] Induction of IL-10- and IFN-γ-producing T-cell responses by autoreactive T-cells expressing human T-cell leukemia virus type I Tax. (2009)
  • Author(s): Takatsuka N, Hasegawa A, Takamori A, Shimizu Y, Kato H, Ohashi T, Amagasa T, Masuda T, Kannagi M.
  • Journal Title: Int Immunol. 21 Pages: 1089-1100
  • NAID: 10027389482
  • Peer Reviewed
• [Journal Article] Detection of multiple sapovirus genotypes and genogroups in oyster-associated outbreaks. (2009)
  • Author(s): Nakagawa-Okamoto R, Arita-Nishida T, Toda S, Kato H, Iwata H, Akiyama M, Nishio O, Kimura H, Noda M, Takeda N, Oka T.
  • Journal Title: Jpn J Infect Dis. 62 Pages: 63-66
  • Peer Reviewed
• [Journal Article] Phylogenic analysis of the genus Leishmania by cytochrome b gene sequencing. (2009)
  • Author(s): Asato Y, Oshiro M, Myint CK, Yamamoto Y, Kato H, Marco JD, Mimori T, Gomez EAL, Hashiguchi Y, Uezato H.
  • Journal Title: Exp Parasitol. 121 Pages: 352-361
  • Peer Reviewed
• [Journal Article] Phylogenic Analysis of the Genus Leishmania by Cytochrome b Gene Sequencing (2009)
  • Author(s): Asato Y
  • Journal Title: Exp.Parasitol 121 Pages: 352-361
  • Peer Reviewed
• [Journal Article] Detection of Multiple Sapovirus Genotypes and Genogroups in Oyster-Associated Outbreaks (2009)
  • Author(s): Nakagawa-Okamoto R
  • Journal Title: Jpn.J.Infect.Dis. 62 Pages: 63-66
  • Peer Reviewed
• [Journal Article] Induction of IL-10- and IFN-γ-producing T-cell responses by autoreactive T-cells expressing human T-cell leukemia virus type I Tax (2009)
  • Author(s): Takatsuka N
  • Journal Title: Int Immunol. 21 Pages: 1089-1100
  • Peer Reviewed
• [Journal Article] Genetic diversity of ribosomal RNA internal transcribed spacer sequences in Lutzomyia species from areas endemic for New World cutaneous leishmaniasis. (2009)
  • Author(s): Kuwahara K
  • Journal Title: Acta Trop. 112 Pages: 131-136
  • Peer Reviewed
• [Journal Article] Functional characterization of a salivary apyrase from the sand fly, Phlebotomus duboscqi, a vector of Leishmania major. (2009)
  • Author(s): Hamasaki R
  • Journal Title: J Insect Physiol. 55 Pages: 1044-1049
  • Peer Reviewed
• [Journal Article] Natural infections of man-biting sand flies by Leishmania and Trypanosoma species in the northern Peruvian Andes.
  • Author(s): Kato H, Gomez EA, Caceres AG, Vargas F, Mimori T, Yamamoto K, Iwata H, Korenaga M, Velez L, Hashiguchi Y.
  • Journal Title: Vector Borne Zoonotic Dis in press
  • Peer Reviewed
• [Presentation] リーシュマニア原虫媒介サシチョウバエLutozomyia ayacuchensisの唾液腺トランスクリプトーム解析 (2010)
  • Author(s): 加藤大智、E.ゴメス、R.ヨキム、上里博、J.ヴァレンズエラ、橋口義久
  • Organizer: 日本獣医学会
  • Place of Presentation: 北海道・帯広畜産大
  • Year and Date: 2010-09-18
• [Presentation] シャーガス病媒介サシガメTriatoma dimidiataの唾液中にみとめられたtriabin様タンパクの機能解析 (2010)
  • Author(s): 石丸由佳、加藤大智、藤田恵、岩田祐之、E.ゴメス、橋口義久
  • Organizer: 日本獣医学会
  • Place of Presentation: 東京・日獣大
  • Year and Date: 2010-03-28
• [Presentation] ペルーにおけるサシチョウバエの遺伝子タイピング法の確立 (2010)
  • Author(s): 藤田恵、加藤大智、A.カセレス、岩田祐之、E.ゴメス、橋口義久
  • Organizer: 日本獣医学会
  • Place of Presentation: 東京・日獣大
  • Year and Date: 2010-03-28
• [Presentation] リーシュマニア原虫媒介サシチョウバエPhlebotomus duboscqiの唾液アピラーゼの機能解析 (2010)
  • Author(s): 加藤大智、濱崎亮一、寺山好美、岩田祐之、J.ヴァレンズエラ
  • Organizer: 日本獣医学会
  • Place of Presentation: 東京・日獣大
  • Year and Date: 2010-03-28
• [Presentation] リーシュマニア原虫媒介サシチョウバエPhiebotomus duboscqiの唾液アピラーゼの機能解析 (2010)
  • Author(s): 加藤大智
  • Organizer: 日本獣医学会
  • Place of Presentation: 東京・日獣大
  • Year and Date: 2010-03-28
• [Presentation] tablishment of a mass screening method of sand fly vectors for Leishmania infection by molecular biological methods (2009)
  • Author(s): 加藤大智
  • Organizer: 日本熱帯医学会
  • Place of Presentation: 沖縄・沖縄コンベンションセンター
  • Year and Date: 2009-10-22
• [Presentation] シャーガス病媒介サシガメTriatoma dimidiataの唾液腺遺伝子転写産物の網羅的解析 (2009)
  • Author(s): 加藤大智、Ryan C.Jochim、佐古田 良、岩田 祐之、Eduardo A.Gomez、Jesus G.Valenzuela、橋口義久
  • Organizer: 日本熱帯医学会
  • Place of Presentation: 沖縄・沖縄コンベンションセンター
  • Year and Date: 2009-10-22
• [Presentation] Establishment of a mass screening method of sand fly vectors for Leishmania infection by molecular biological methods (2009)
  • Author(s): 加藤大智
  • Organizer: 日本熱帯医学会
  • Place of Presentation: 沖縄・沖縄コンベンションセンター
  • Year and Date: 2009-10-22
• [Presentation] サシガメTriatoma (T.) dimidiataの唾液腺トランスクリプトーム解析 (2009)
  • Author(s): 加藤大智、R.ヨキム、佐古田良、岩田祐之、E.ゴメス、J.ヴァレンズエラ、橋口義久
  • Organizer: 日本獣医学会
  • Place of Presentation: 鳥取・とりぎん文化会館
  • Year and Date: 2009-09-26
• [Presentation] Lutzomyia属サシチョウバエrRNA遺伝子ITS領域の遺伝子多様性 (2009)
  • Author(s): 桑原慶、加藤大智、E.ゴメス、上里博、三森龍之、山本雄一、M.カルボピーニャ、A.カセレス、岩田祐之、橋口義久
  • Organizer: 日本獣医学会
  • Place of Presentation: 鳥取・とりぎん文化会館
  • Year and Date: 2009-09-26
• [Presentation] Luzomyia属サシチョウバエrRNA遺伝子ITS領域の遺伝子多様性 (2009)
  • Author(s): 桑原慶
  • Organizer: 日本獣医学会
  • Place of Presentation: 鳥取・とりぎん文化会館
  • Year and Date: 2009-09-26
• [Presentation] サシガメTriatoma dimidiataの唾液腺トランスクリプトーム解析 (2009)
  • Author(s): 加藤大智、Ryan C.Jochim、佐古田 良、岩田 祐之、Eduardo A.Gomez、Jesus G.Valenzuela、橋口義久
  • Organizer: 日本衛生動物学会
  • Place of Presentation: 高松・サンポートホール高松
  • Year and Date: 2009-04-03
• [Remarks] ホームページ等
  • URL: http://www.vet.agr.yamaguchi-u.ac.jp/member/hkato/kato_p.htm