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Elucidation of follicle selection system using bovine follicle culture and application for follicle and oocyte culture of wildlife

Research Project

Project/Area Number 21780286
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Clinical veterinary science
Research InstitutionHokkaido University

Principal Investigator

NAGANO Masashi  Hokkaido University, 大学院・獣医学研究科, 准教授 (70312402)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords臨床繁殖 / 産科 / 体外受精 / 卵胞発育 / 猫科動物 / 獣医学 / 動物 / 卵胞 / 卵子 / 体外培養
Research Abstract

For clarification of mechanisms of follicular development and degeneration, growing oocytes were collected from bovine and feline ovaries and cultured in vitro. It was suggested that high concentration of follicular stimulating hormone inhibited the oocyte growth in vitro, and estradiol was essential for oocyte and follicular development. Fibroblast growth factor 7 stimulated oocyte growth and granulosa cell proliferation in early culture stage, but it did not have previous effects on growing oocytes in late culture stage.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (12 results)

All 2011 2010 2009 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Prediction of maturational competence of feline oocytes using supravital staining of cumulus cells by propidium iodide.2011

    • Author(s)
      Uchikura, K., Nagano, M., Hishinuma, M.
    • Journal Title

      Zygote in press

    • NAID

      120004448499

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of ovarian status and follicular diameter on maturational ability of domestic cat oocytes.2011

    • Author(s)
      Uchikura, K., Nagano, M., Hishinuma, M.
    • Journal Title

      The Journal of Veterinary Medical Science in press

    • NAID

      130000444665

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 野生猫科動物への人工繁殖技術の応用2011

    • Author(s)
      永野昌志, 内倉健造
    • Journal Title

      北海道獣医師会雑誌 55

      Pages: 121-127

    • Related Report
      2010 Final Research Report
  • [Journal Article] Evaluation of follicular development and oocyte quality in prepubertal cats.2010

    • Author(s)
      Uchikura, K., Nagano, M., Hishinuma, M.
    • Journal Title

      Reproduction in Domestic Animals 45

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of follicular development and oocyte quality in prepubertal cats2010

    • Author(s)
      Uchikura, Kenzo.
    • Journal Title

      Reproduction in Domestic Animals

      Volume: 45

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ATP content of mouse oocytes matured in vivo and in vitro under different oxygen tensions2010

    • Author(s)
      Adam, A.A.Gadil.
    • Journal Title

      Journal of Natural Resources and Environmental Studies

      Volume: 5 Pages: 33-40

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microencapsulation of canine sperm and its preservation at 4℃2010

    • Author(s)
      Shah, S., Nagano, M., Yamashita, Y., Hishinuma, M.
    • Journal Title

      Theriogenology 73

      Pages: 560-567

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transforming growth factor-α in a defined medium during in vitro maturation of porcine oocytes improves their developmental competence and intracellular ultrastructure2009

    • Author(s)
      Mito, T., Yoshioka, K., Nagano, M., Suzuki, C., Yamashita, S., Hoshi, H.
    • Journal Title

      Theriogenology 72

      Pages: 841-850

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 猫卵子の体外成熟培養に関する研究2010

    • Author(s)
      永野昌志
    • Organizer
      第149回日本獣医学会学術集会
    • Place of Presentation
      日本獣医生命科学大学(東京)
    • Year and Date
      2010-03-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 猫卵子の体外成熟培養に関する研究2010

    • Author(s)
      永野昌志
    • Organizer
      日本獣医学会
    • Place of Presentation
      日本獣医生命科学大学(東京)
    • Year and Date
      2010-03-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 初期胞状卵胞由来牛卵子の体外発育に及ぼす卵胞刺激ホルモン(FSH)の影響2009

    • Author(s)
      郡司庸介、永野昌志、寺野貴之、山下泰尚、菱沼貢
    • Organizer
      関西畜産学会
    • Place of Presentation
      鳥取大学(嶋取)
    • Year and Date
      2009-08-28
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.vetmed.hokudai.ac.jp/organization/theriogenol/index.html

    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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