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Identification and application of new genes involved in glycosylation in koji mold

Research Project

Project/Area Number 21780313
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied molecular and cellular biology
Research InstitutionSojo University

Principal Investigator

OKA Takuji  Sojo University, 生物生命学部, 准教授 (50510690)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords糸状菌 / 麹菌 / 細胞壁 / 糖鎖 / 糖鎖工学 / 糖鎖リモデリング / ガラクトフラナン
Research Abstract

Most of the enzymes from Aspergillus, including koji mold, are glycosylated, which improves both the functioning and efficacy of these enzymes. Although galactofuranose and glucose residues have been observed to be attached to the O-glycans and N-glycans of proteins in Aspergillus, the glycosyltransferase genes involved in the biosynthesis of these sugar residues have not yet been identified and the function of these sugar moieties also is not yet known1).
The ability of filamentous fungi to secrete large quantities of proteins means that they are well suited for use as expression hosts for extragenous genes in industrial applications. However, since the sugar moieties of glycoproteins from filamentous fungi are antigenic in humans, they cannot be used for medicinal applications, such as antibody preparation. Consequently, a method for altering the structure of these sugar moieties needs to be developed so that a protein expression system using filamentous fungi can be developed.
We therefore sought to identify the genes involved in the biosynthesis of these sugar moieties in filamentous fungi, and to elucidate the function of these sugar moieties in fungal growth and protein secretion.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (12 results)

All 2010 2009

All Presentation (12 results)

  • [Presentation] Asperglllus nidulans における分生子形成に関与する機能未知膜タンパク質の解析2010

    • Author(s)
      石井千尋, 小町裕司, 浴野圭輔, 二神泰基, 竹川薫, 後藤正利, 野村善幸, 岡拓二
    • Organizer
      (崇城大・生物生命・応微工,九大・院・農)日本農芸化学会2010年度九州支部大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-09-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Asperglllus nidulans のガラクトマンナン合成に関与する遺伝子の機能解析2010

    • Author(s)
      小町裕司, 浴野圭輔, 二神泰基, 竹川薫, 後藤正利, 野村善幸, 岡拓二
    • Organizer
      (1崇城大・生物生命・応微工、九大・院・農)日本農芸化学会2010年度九州支部大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-09-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Aspergillus nidulansにおける分生子形成に関与する機能未知膜タンパク質の解析2010

    • Author(s)
      石井千尋, 小町裕司, 浴野圭輔, 二神泰基, 竹川薫, 後藤正利, 野村善幸, 岡拓二
    • Organizer
      日本農芸化学会 2010年度九州支部大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-09-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Aspergillus nldulansのガラクトマンナン合成に関与する遺伝子の機能解析2010

    • Author(s)
      小町裕司, 浴野圭輔, 二神泰基, 竹川薫, 後藤正利, 野村善幸, 岡拓二
    • Organizer
      日本農芸化学会 2010年度九州支部大会
    • Place of Presentation
      熊本
    • Year and Date
      2010-09-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Asperglllus nidulans の2つのUDP-グルコースコース/UDP-ガラクトース4-エピメラーゼ遺伝子の機能解析2010

    • Author(s)
      岡拓二、浴野圭輔、二神泰基、竹川薫、後藤正利、野村善幸
    • Organizer
      (崇城大・生物生命・応微工、九大院・農院)日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Aspergillus nidulans の2つのUDP-グルコース/UDP-ガラクトース4-エピメラーゼ遺伝子の機能解析2010

    • Author(s)
      岡拓二、浴野圭輔、二神泰基、竹川薫、後藤正利、野村善幸
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] Asperglllus nidulans のガラクトマンナン生合成に関与する遺伝子の同定と機能解析2010

    • Author(s)
      小町裕司, 浴野圭輔, 二神泰基, 竹川薫, 後藤止利, 野村善幸, 岡拓二
    • Organizer
      (崇城大・生物生命・応微工、九大・院・農)第10回糸状菌コンファレンス
    • Place of Presentation
      広島
    • Related Report
      2010 Final Research Report
  • [Presentation] Aspergillus nidulansのガラクトマンナン生合成に関与する遺伝子の同定と機能解析2010

    • Author(s)
      小町裕司, 浴野圭輔, 二神泰基, 竹川薫, 後藤正利, 野村善幸, 岡拓二
    • Organizer
      第10回糸状菌コンファレンス
    • Place of Presentation
      広島
    • Related Report
      2010 Annual Research Report
  • [Presentation] Aspergillus nidulans のGT31ファミリーに属する新規糖転移酵素遺伝子の機能解析2009

    • Author(s)
      小町裕司、岡拓二、竹川薫、後藤正利、野村善幸
    • Organizer
      糸状菌コンファレンス
    • Place of Presentation
      東京大学弥生講堂
    • Year and Date
      2009-11-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] Aspergillus nidulans のGT31ファミリーに属する機能未知糖転移酵素遺伝子の機能解析2009

    • Author(s)
      小町裕司、岡拓二、竹川薫、後藤正利、野村善幸
    • Organizer
      日本農芸化学会西日本支部大会
    • Place of Presentation
      琉球大学
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] Asperglllus nidulans のGT31ファミリーに属する新規糖転移酵素遺伝子の機能解析2009

    • Author(s)
      小町裕司、岡拓二、竹川薫、後藤止利、野村善幸1
    • Organizer
      (崇城大・生物生命・応微.工、九大・院・農)第9回糸状菌コンファレンス
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] Asperglllus nidulans のGT31ファミリーに属する機能未知糖転移酵素遺伝子の機能解析2009

    • Author(s)
      小町裕司, 岡拓二, 竹川薫, 後藤正利, 野村善幸
    • Organizer
      (崇城大・生物生命・応微工,九大・院・農)日本農芸化学会西口本支部大会
    • Place of Presentation
      沖縄
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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